Detail Information for IndEnz0002018727
IED ID IndEnz0002018727
Enzyme Type ID protease018727
Protein Name Probable cytosol aminopeptidase
EC 3.4.11.1
Leucine aminopeptidase
LAP
EC 3.4.11.10
Leucyl aminopeptidase
Gene Name pepA CTC_01866
Organism Clostridium tetani (strain Massachusetts / E88)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Clostridia Eubacteriales Clostridiaceae Clostridium Clostridium tetani Clostridium tetani (strain Massachusetts / E88)
Enzyme Sequence MKFHIGKDGEVKIILVYKNAEKVEEANDLYSYLKEKELFKGDLGEVYSHISHSEDKFILLGIGEKEKTTANSLRKAFFNAGKELMKFKVTSIKISIPMFKNLKYESIVQSITEGLLQSEYSFEKYLTKKKISPSIKNVYLDILEEKKEETINTINESKNLIEGIFLARNLVNEPAIYMTPTVLANNAKNQLESLGVEVKIYGKEKIKELGMEAFLAVSKGSFEESQLIVMNYKGNSNSDKKLALVGKGLTYDSGGYSIKPTSSMINMHSDMAGSASVIGAMKAIAMSKLEVNVVAIVAACENMISGGAYKPGDIISSMSGKTIEILNTDAEGRLTLADALWYAVDVVKADKIIDIATLTGACVTALGSINTGAITNNSYLMDNVKKASECAGEPVWEFPNNDEYKELIKGTFGDLKNSSGREAGAITAGMFLQEFVGNTPWVHLDVAGTAYLSSKNGYLQKGATGVPVKTLYYLAKGFKNK
Enzyme Length 481
Uniprot Accession Number Q893F8
Absorption
Active Site ACT_SITE 259; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 333; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181};
DNA Binding
EC Number 3.4.11.1; 3.4.11.10
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (7)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 52,612
Kinetics
Metal Binding METAL 247; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 252; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 252; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 270; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 329; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 331; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 331; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Rhea ID
Cross Reference Brenda