Detail Information for IndEnz0002018737
IED ID IndEnz0002018737
Enzyme Type ID protease018737
Protein Name Probable cytosol aminopeptidase
EC 3.4.11.1
Leucine aminopeptidase
LAP
EC 3.4.11.10
Leucyl aminopeptidase
Gene Name pepA ERGA_CDS_06600
Organism Ehrlichia ruminantium (strain Gardel)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Alphaproteobacteria Rickettsiales Anaplasmataceae Ehrlichia canis group Ehrlichia ruminantium (Cowdria ruminantium) Ehrlichia ruminantium (strain Gardel)
Enzyme Sequence MINVSFLGLMSGISVLLKTTVIVVGIFEGSNHLEDNGALEGYNDKIMEIVNGYQSFDGKFAEVLPIIGLEKDFPVVVVIGLGKSEDFDENKALKVGGVIYSELNRMKVPDASIVINTDSNVSANIGYGALLRSFKFDKYFVEKKDKNSVYLNKLVLFSKNDPQEVTALFNDLKAEGESIFLARSFVSEPPNILYPETYAQMIYEELSKVGVTVEVFDEDYMKANQMMALLGVGQGSAKKSRLVVMKWNGGDESESPIAFVGKGVTFDTGGISLKPSKGMWDMKYDMAGSASVVGIMRTLAARKAKVNAVGVVGLVENSVDGNAQRPSDVVISMSGQTIEVLNTDAEGRLVLADALWYTQEMFTPKLMVDLATLTGAVVVALGNNQYAGLFSNDDAIANQLIVAGNESGEKLWRLPLDEAYDKLIDSSIADMQNISTKGYGADSITAAQFLQRFVNGVPWVHLDIAGMAWDYEGTEICPKGATGFGVRLLNRFVSKYYESH
Enzyme Length 500
Uniprot Accession Number Q5FFZ5
Absorption
Active Site ACT_SITE 274; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 348; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181};
DNA Binding
EC Number 3.4.11.1; 3.4.11.10
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (7)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 54,435
Kinetics
Metal Binding METAL 262; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 267; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 267; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 285; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 344; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 346; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 346; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Rhea ID
Cross Reference Brenda