| IED ID | IndEnz0002018750 |
| Enzyme Type ID | protease018750 |
| Protein Name |
AFG3-like protein spg-7 EC 3.4.24.- |
| Gene Name | spg-7 Y47G6A.10 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MWRLYRKTPKQLRNSVESCFKSVNSRQFSVSSFSAVAKDDKSVLKQQEVLHLLAKDQRFEERFFNQVQQTIRYFASKPNDLKKFFRKEASTDNKESSSSSDKKDSKGNKGEDNNPFNQFPGGWQQIAVSVGILLALYAFMDYQSYREISWKEFYSDFLEAGLVERLEVVDKRWVRIVSSSGKYAGQTCYFNIGSVDSFERSLGAAQHHLQYDADRQIPVLYKSEFNFKREIPNLISVAFPLLFGYYIYRMLKGGGAAGGAGRAGGGGLGGMFGGFGQSTARVINREDIKVKFADVAGCEEAKIEIMEFVNFLKNPQQYKDLGAKIPKGAILTGPPGTGKTLLAKATAGEANVPFITVSGSEFLEMFVGVGPARVRDMFSMARKNSPCILFIDEIDAVGRKRGGKGGMGGHSEQENTLNQLLVEMDGFTTDESSVIVIAATNRVDILDSALLRPGRFDRQIYVPVPDIKGRASIFRVHLGPLRTSLDKTVLSRKLAAHTPGFSGADISNVCNEAALIAARDANHEISNKHFEQAIERVVAGMEKKTQVLQKEEKKTVAYHEAGHAIAGWFLQHADPLLKVSIIPRGKGLGYAQYLPKEQYLYSKDQLLDRMCMTLGGRVAEEIFFGRITTGAQDDLQKVTQMAYSQVVKFGMSEKVGPLSFETPAPGEMAFDKPYSEATAQLIDQEVRDLVMNALRRTRDLLLEKRSDIERVALRLLEKEILNREDMIELVGKRPFVEKNTYEEMVSGTGGLDENVELPKGLENWNKESEKKKKDEEKKKNDE |
| Enzyme Length | 782 |
| Uniprot Accession Number | Q9N3T5 |
| Absorption | |
| Active Site | ACT_SITE 560; /evidence=ECO:0000250|UniProtKB:Q9WZ49 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Acts as a component of the m-AAA protease complex which is an ATP-dependent metalloprotease mediating degradation of non-assembled mitochondrial inner membrane proteins (By similarity). The complex is necessary for the assembly of mitochondrial respiratory chain and ATPase complexes (By similarity). Functions both in post-translational assembly and in the turnover of mistranslated or misfolded polypeptides (PubMed:22700657, PubMed:25274306, PubMed:25773600). Plays a role in male tail tip morphogenesis (PubMed:21408209). {ECO:0000250|UniProtKB:P39925, ECO:0000269|PubMed:21408209, ECO:0000305|PubMed:22700657, ECO:0000305|PubMed:25274306, ECO:0000305|PubMed:25773600}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 333..340; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00499 |
| Features | Active site (1); Chain (1); Compositional bias (2); Metal binding (3); Nucleotide binding (1); Region (2); Topological domain (3); Transmembrane (2) |
| Keywords | ATP-binding;Hydrolase;Membrane;Metal-binding;Metalloprotease;Mitochondrion;Mitochondrion inner membrane;Nucleotide-binding;Protease;Reference proteome;Transmembrane;Transmembrane helix;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250|UniProtKB:P39925}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P39925}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10778742; 11099033; 11231151; 11381264; 11483502; 14551910; 15084750; 15280428; 15489339; 15791247; 16816413; 16854972; 17164286; 17411345; 17704769; 17925224; 19343510; 20089839; 20188671; 20439774; 20439776; 21085631; 21177967; 21367940; 21529718; 22220943; 22267497; 22286215; 22347378; 22500807; 22560298; 22719267; 23800452; 24884423; 25215496; 25487147; 25979236; 26913604; 27129311; 27459203; 29348603; 30929899; 6593563; |
| Motif | |
| Gene Encoded By | |
| Mass | 87,469 |
| Kinetics | |
| Metal Binding | METAL 559; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:Q9WZ49; METAL 563; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:Q9WZ49; METAL 634; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:Q9WZ49 |
| Rhea ID | |
| Cross Reference Brenda |