Detail Information for IndEnz0002018752
IED ID IndEnz0002018752
Enzyme Type ID protease018752
Protein Name Probable cytosol aminopeptidase
EC 3.4.11.1
Leucine aminopeptidase
LAP
EC 3.4.11.10
Leucyl aminopeptidase
Gene Name pepA PSPA7_1282
Organism Pseudomonas aeruginosa (strain PA7)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa group Pseudomonas aeruginosa Pseudomonas aeruginosa (strain PA7)
Enzyme Sequence MEFLVKSVRPETLKTATLVLAVGEGRKLGASAKAVDDATGGAIGAVLKRGDLAGKVGQTLLLQNLPNLKAERVLLVGAGKERELGDRQYRKLASAVLSTLKGLAGADAVLALGDLAVKGRDAHAKARLLVETLADGLYVFDRYKSQKAEPLKLKKLTLLADKADSAAVEQGSKEAQAIANGMALTRDLGNLPPNVCHPTFLGEQAKALAKEFKGLKVEVHDEQKLRELGMGSFLAVAQGSEQPPRLIVLQYNGAKKDQAPHVLVGKGITFDTGGISLKPGLGMDEMKFDMCGAASVFGTFRAVLELQLPINLVGLLACAENMPSGGATRPGDIVTTMSGQTVEILNTDAEGRLVLCDALTYAERFKPQSVVDIATLTGACIVALGSNTSGLMGNNEALVRQLLKAGEFADDRAWQLPLFDEYQEQLDSPFADIANIGGPKAGTITAGCFLSRFAKKYHWAHLDIAGTAWISGGKDKGATGRPVPLLTQYLLERAK
Enzyme Length 495
Uniprot Accession Number A6V0T2
Absorption
Active Site ACT_SITE 278; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 352; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181};
DNA Binding
EC Number 3.4.11.1; 3.4.11.10
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (7)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 52,395
Kinetics
Metal Binding METAL 266; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 271; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 271; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 289; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 348; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 350; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 350; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Rhea ID
Cross Reference Brenda