| IED ID | IndEnz0002018753 |
| Enzyme Type ID | protease018753 |
| Protein Name |
Mitochondrial respiratory chain complexes assembly protein AFG3 EC 3.4.24.- ATPase family gene 3 protein Tat-binding homolog 10 |
| Gene Name | AFG3 YTA10 YER017C |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MMMWQRYARGAPRSLTSLSFGKASRISTVKPVLRSRMPVHQRLQTLSGLATRNTIHRSTQIRSFHISWTRLNENRPNKEGEGKNNGNKDNNSNKEDGKDKRNEFGSLSEYFRSKEFANTMFLTIGFTIIFTLLTPSSNNSGDDSNRVLTFQDFKTKYLEKGLVSKIYVVNKFLVEAELVNTKQVVSFTIGSVDIFEEQMDQIQDLLNIPPRDRIPIKYIERSSPFTFLFPFLPTIILLGGLYFITRKINSSPPNANGGGGGGLGGMFNVGKSRAKLFNKETDIKISFKNVAGCDEAKQEIMEFVHFLKNPGKYTKLGAKIPRGAILSGPPGTGKTLLAKATAGEANVPFLSVSGSEFVEMFVGVGASRVRDLFTQARSMAPSIIFIDEIDAIGKERGKGGALGGANDEREATLNQLLVEMDGFTTSDQVVVLAGTNRPDVLDNALMRPGRFDRHIQIDSPDVNGRQQIYLVHLKRLNLDPLLTDDMNNLSGKLATLTPGFTGADIANACNEAALIAARHNDPYITIHHFEQAIERVIAGLEKKTRVLSKEEKRSVAYHEAGHAVCGWFLKYADPLLKVSIIPRGQGALGYAQYLPPDQYLISEEQFRHRMIMALGGRVSEELHFPSVTSGAHDDFKKVTQMANAMVTSLGMSPKIGYLSFDQNDGNFKVNKPFSNKTARTIDLEVKSIVDDAHRACTELLTKNLDKVDLVAKELLRKEAITREDMIRLLGPRPFKERNEAFEKYLDPKSNTEPPEAPAATN |
| Enzyme Length | 761 |
| Uniprot Accession Number | P39925 |
| Absorption | |
| Active Site | ACT_SITE 559; /evidence=ECO:0000250|UniProtKB:Q9WZ49 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Acts as a component of the m-AAA protease complex which is a ATP-dependent metalloprotease mediating degradation of non-assembled mitochondrial inner membrane proteins. The complex is necessary for the assembly of mitochondrial respiratory chain and ATPase complexes. Function both in post-translational assembly and in the turnover of mistranslated or misfolded polypeptides. {ECO:0000269|PubMed:8681382, ECO:0000269|PubMed:9707443}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 328..335; /note=ATP; /evidence=ECO:0000255 |
| Features | Active site (1); Chain (1); Compositional bias (1); Metal binding (3); Mutagenesis (1); Nucleotide binding (1); Region (1); Sequence conflict (1); Topological domain (3); Transmembrane (2) |
| Keywords | ATP-binding;Hydrolase;Membrane;Metal-binding;Metalloprotease;Mitochondrion;Mitochondrion inner membrane;Nucleotide-binding;Protease;Reference proteome;Transmembrane;Transmembrane helix;Zinc |
| Interact With | P40341 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000269|PubMed:8681382}; Multi-pass membrane protein {ECO:0000269|PubMed:8681382}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10207067; 10453730; 10782097; 10882099; 10906275; 10996302; 11212342; 11251115; 11283351; 11805837; 11996120; 12191771; 12228240; 12417197; 12791261; 12880202; 14623864; 15205678; 15328540; 16239145; 16429126; 16527490; 16647881; 16725216; 16778770; 17245427; 17615298; 18719252; 19019989; 19221197; 19453275; 19503795; 19536198; 19748349; 19748354; 20208537; 20950655; 21147776; 21439406; 21447998; 21610694; 21779004; 21902802; 22001671; 22022284; 22094426; 22172993; 22498346; 22663076; 22910375; 23167605; 23283966; 23509072; 23837470; 24119093; 24648523; 25927548; 26030272; 26035862; 26415097; 26456335; 27398316; 29030426; 7589436; 7926051; 7926052; 8641436; 8690083; 8753648; 8810243; 8861950; 8988248; 9106654; 9149530; 9276437; 9635427; 9857067; |
| Motif | |
| Gene Encoded By | |
| Mass | 84,544 |
| Kinetics | |
| Metal Binding | METAL 558; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:Q9WZ49; METAL 562; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:Q9WZ49; METAL 634; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:Q9WZ49 |
| Rhea ID | |
| Cross Reference Brenda |