Detail Information for IndEnz0002018753
IED ID IndEnz0002018753
Enzyme Type ID protease018753
Protein Name Mitochondrial respiratory chain complexes assembly protein AFG3
EC 3.4.24.-
ATPase family gene 3 protein
Tat-binding homolog 10
Gene Name AFG3 YTA10 YER017C
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Enzyme Sequence MMMWQRYARGAPRSLTSLSFGKASRISTVKPVLRSRMPVHQRLQTLSGLATRNTIHRSTQIRSFHISWTRLNENRPNKEGEGKNNGNKDNNSNKEDGKDKRNEFGSLSEYFRSKEFANTMFLTIGFTIIFTLLTPSSNNSGDDSNRVLTFQDFKTKYLEKGLVSKIYVVNKFLVEAELVNTKQVVSFTIGSVDIFEEQMDQIQDLLNIPPRDRIPIKYIERSSPFTFLFPFLPTIILLGGLYFITRKINSSPPNANGGGGGGLGGMFNVGKSRAKLFNKETDIKISFKNVAGCDEAKQEIMEFVHFLKNPGKYTKLGAKIPRGAILSGPPGTGKTLLAKATAGEANVPFLSVSGSEFVEMFVGVGASRVRDLFTQARSMAPSIIFIDEIDAIGKERGKGGALGGANDEREATLNQLLVEMDGFTTSDQVVVLAGTNRPDVLDNALMRPGRFDRHIQIDSPDVNGRQQIYLVHLKRLNLDPLLTDDMNNLSGKLATLTPGFTGADIANACNEAALIAARHNDPYITIHHFEQAIERVIAGLEKKTRVLSKEEKRSVAYHEAGHAVCGWFLKYADPLLKVSIIPRGQGALGYAQYLPPDQYLISEEQFRHRMIMALGGRVSEELHFPSVTSGAHDDFKKVTQMANAMVTSLGMSPKIGYLSFDQNDGNFKVNKPFSNKTARTIDLEVKSIVDDAHRACTELLTKNLDKVDLVAKELLRKEAITREDMIRLLGPRPFKERNEAFEKYLDPKSNTEPPEAPAATN
Enzyme Length 761
Uniprot Accession Number P39925
Absorption
Active Site ACT_SITE 559; /evidence=ECO:0000250|UniProtKB:Q9WZ49
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.24.-
Enzyme Function FUNCTION: Acts as a component of the m-AAA protease complex which is a ATP-dependent metalloprotease mediating degradation of non-assembled mitochondrial inner membrane proteins. The complex is necessary for the assembly of mitochondrial respiratory chain and ATPase complexes. Function both in post-translational assembly and in the turnover of mistranslated or misfolded polypeptides. {ECO:0000269|PubMed:8681382, ECO:0000269|PubMed:9707443}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding NP_BIND 328..335; /note=ATP; /evidence=ECO:0000255
Features Active site (1); Chain (1); Compositional bias (1); Metal binding (3); Mutagenesis (1); Nucleotide binding (1); Region (1); Sequence conflict (1); Topological domain (3); Transmembrane (2)
Keywords ATP-binding;Hydrolase;Membrane;Metal-binding;Metalloprotease;Mitochondrion;Mitochondrion inner membrane;Nucleotide-binding;Protease;Reference proteome;Transmembrane;Transmembrane helix;Zinc
Interact With P40341
Induction
Subcellular Location SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000269|PubMed:8681382}; Multi-pass membrane protein {ECO:0000269|PubMed:8681382}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10207067; 10453730; 10782097; 10882099; 10906275; 10996302; 11212342; 11251115; 11283351; 11805837; 11996120; 12191771; 12228240; 12417197; 12791261; 12880202; 14623864; 15205678; 15328540; 16239145; 16429126; 16527490; 16647881; 16725216; 16778770; 17245427; 17615298; 18719252; 19019989; 19221197; 19453275; 19503795; 19536198; 19748349; 19748354; 20208537; 20950655; 21147776; 21439406; 21447998; 21610694; 21779004; 21902802; 22001671; 22022284; 22094426; 22172993; 22498346; 22663076; 22910375; 23167605; 23283966; 23509072; 23837470; 24119093; 24648523; 25927548; 26030272; 26035862; 26415097; 26456335; 27398316; 29030426; 7589436; 7926051; 7926052; 8641436; 8690083; 8753648; 8810243; 8861950; 8988248; 9106654; 9149530; 9276437; 9635427; 9857067;
Motif
Gene Encoded By
Mass 84,544
Kinetics
Metal Binding METAL 558; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:Q9WZ49; METAL 562; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:Q9WZ49; METAL 634; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:Q9WZ49
Rhea ID
Cross Reference Brenda