Detail Information for IndEnz0002018769
IED ID IndEnz0002018769
Enzyme Type ID protease018769
Protein Name Calpain-2 catalytic subunit
EC 3.4.22.53
Calcium-activated neutral proteinase 2
CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
M-calpain
Fragment
Gene Name CAPN2
Organism Sus scrofa (Pig)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Suina Suidae (pigs) Sus Sus scrofa (Pig)
Enzyme Sequence YPNTFWMNPQYLIKLEEEDEDQEDGESGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELTGQTNIHLSKNFFLTHRARERSDTFINLREVLNRFKLPPGEYILVPSTFEPNKDGDFCIRVFSEKKADYQVVDDEIEADLEENDASEDDIDDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCRIMVDMLDSDGSAKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKLPCQLHQVIVARFADDQLIIDFDNFVRCLVRLETLFRISKQLDSENTGTIELDLISWLCFSVL
Enzyme Length 324
Uniprot Accession Number P43367
Absorption
Active Site
Activity Regulation ACTIVITY REGULATION: Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.
Binding Site
Calcium Binding CA_BIND 209..220; /note=1; CA_BIND 239..250; /note=2
catalytic Activity CATALYTIC ACTIVITY: Reaction=Broad endopeptidase specificity.; EC=3.4.22.53;
DNA Binding
EC Number 3.4.22.53
Enzyme Function FUNCTION: Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs. {ECO:0000250|UniProtKB:O08529, ECO:0000250|UniProtKB:P17655}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Calcium binding (2); Chain (1); Domain (2); Metal binding (16); Non-terminal residue (1); Region (3); Sequence conflict (1)
Keywords Calcium;Cell membrane;Cytoplasm;Hydrolase;Membrane;Metal-binding;Protease;Reference proteome;Repeat;Thiol protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Cell membrane {ECO:0000250}. Note=Translocates to the plasma membrane upon Ca(2+) binding. {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 37,809
Kinetics
Metal Binding METAL 166; /note=Calcium 5; via carbonyl oxygen; /evidence=ECO:0000250; METAL 169; /note=Calcium 5; /evidence=ECO:0000250; METAL 171; /note=Calcium 5; via carbonyl oxygen; /evidence=ECO:0000250; METAL 176; /note=Calcium 5; /evidence=ECO:0000250; METAL 209; /note=Calcium 6; /evidence=ECO:0000250; METAL 211; /note=Calcium 6; /evidence=ECO:0000250; METAL 213; /note=Calcium 6; via carbonyl oxygen; /evidence=ECO:0000250; METAL 215; /note=Calcium 6; via carbonyl oxygen; /evidence=ECO:0000250; METAL 220; /note=Calcium 6; /evidence=ECO:0000250; METAL 239; /note=Calcium 7; /evidence=ECO:0000250; METAL 241; /note=Calcium 7; /evidence=ECO:0000250; METAL 243; /note=Calcium 7; via carbonyl oxygen; /evidence=ECO:0000250; METAL 245; /note=Calcium 7; via carbonyl oxygen; /evidence=ECO:0000250; METAL 250; /note=Calcium 7; /evidence=ECO:0000250; METAL 282; /note=Calcium 1; /evidence=ECO:0000250; METAL 285; /note=Calcium 1; /evidence=ECO:0000250
Rhea ID
Cross Reference Brenda 3.4.22.53;