Detail Information for IndEnz0002018794
IED ID IndEnz0002018794
Enzyme Type ID protease018794
Protein Name Probable cytosol aminopeptidase
EC 3.4.11.1
Leucine aminopeptidase
LAP
EC 3.4.11.10
Leucyl aminopeptidase
Gene Name pepA A1G_01050
Organism Rickettsia rickettsii (strain Sheila Smith)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Alphaproteobacteria Rickettsiales Rickettsiaceae Rickettsieae Rickettsia spotted fever group Rickettsia rickettsii Rickettsia rickettsii (strain Sheila Smith)
Enzyme Sequence MLNVNFVNEASSTNQGLVVFIDEQLKLDSNLIGLDQQHHGLISKTIQNKLQFTGKYGQIKVIPSVIKSGEVRYLIIAGLGNEEKLTEAKIEELGGKILQHATSCKISTIGLKITNRISRFTSQTFASLVASGAFLASYRFDKYRTTLKEAEKFAVESIEIFTDNSTETAKLFEIKKLIAEAVFFTRDISNEPSNIKTPQVYAERIVDRLEPLGVDVDVIGEREMKNLGMGALLGVGQGSQNESKLVVMEYKGGSKDVPTIALVGKGVIFDTGGISLKPSSDMHLMRYDMGGSAAVVGTIIAVAGQKLPVNIVGVVGLVENMLSGNAQRPGDVVTTMSGQTAEVLNTDAEGRLVLADAVWYAQEKFKPKCVIDVATLTGAITIALGNTYAGCFSNNDELADKLIKVGEEVNEKLWRMPLHDEYDAMIHSDIADMANIANVPRAAGSCIAAHFIKRFIKDGVDWAHLDIAGVANSNKASALGPKGAVGYGVRLLEKFIKEYT
Enzyme Length 500
Uniprot Accession Number A8GQW7
Absorption
Active Site ACT_SITE 277; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 351; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181};
DNA Binding
EC Number 3.4.11.1; 3.4.11.10
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (7)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 54,065
Kinetics
Metal Binding METAL 265; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 270; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 270; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 288; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 347; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 349; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 349; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Rhea ID
Cross Reference Brenda