| IED ID | IndEnz0002018796 |
| Enzyme Type ID | protease018796 |
| Protein Name |
Aminopeptidase P2 AtAPP2 EC 3.4.11.9 |
| Gene Name | APP2 At3g05350 T12H1.32 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MIPLTLSSPSLNRLVLSTSRYSHSLFLSNFNSLSLIHRKLPYKPLFGARCHASSSSSSSSSFTAKSSKEIRKAQTKVVVDEKLSSIRRLFSEPGVGIDAYIIPSQDAHQSEFIAECYARRAYISGFTGSAGTAVVTKDKAALWTDGRYFLQAEKQLNSSWILMRAGNPGVPTASEWIADVLAPGGRVGIDPFLFSADAAEELKEVIAKKNHELVYLYNVNLVDEIWKDSRPKPPSRQIRIHDLKYAGLDVASKLLSLRNQIMDAGTSAIVISMLDEIAWVLNLRGSDVPHSPVMYAYLIVEVDQAQLFVDNSKVTVEVKDHLKNAGIELRPYDSILQGIDSLAARGAQLLMDPSTLNVAIISTYKSACERYSRNFESEAKVKTKFTDSSSGYTANPSGIYMQSPISWAKAIKNDAELKGMKNSHLRDAAALAHFWAWLEEEVHKNANLTEVDVADRLLEFRSMQDGFMDTSFDTISGSGANGAIIHYKPEPESCSRVDPQKLFLLDSGAQYVDGTTDITRTVHFSEPSAREKECFTRVLQGHIALDQAVFPEGTPGFVLDGFARSSLWKIGLDYRHGTGHGVGAALNVHEGPQSISFRYGNMTPLQNGMIVSNEPGYYEDHAFGIRIENLLHVRDAETPNRFGGATYLGFEKLTFFPIQTKMVDVSLLSDTEVDWLNSYHAEVWEKVSPLLEGSTTQQWLWNNTRPLAKP |
| Enzyme Length | 710 |
| Uniprot Accession Number | Q8RY11 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 147; /note=Substrate; /evidence=ECO:0000250|UniProtKB:O44750; BINDING 486; /note=Substrate; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:O44750; BINDING 580; /note=Substrate; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:O44750; BINDING 589; /note=Substrate; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:O44750; BINDING 614; /note=Substrate; /evidence=ECO:0000250|UniProtKB:O44750 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.; EC=3.4.11.9; Evidence={ECO:0000250|UniProtKB:F4JQH3}; |
| DNA Binding | |
| EC Number | 3.4.11.9 |
| Enzyme Function | FUNCTION: Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro. {ECO:0000250|UniProtKB:Q9VJG0}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Binding site (5); Chain (1); Erroneous gene model prediction (1); Metal binding (7); Transit peptide (1) |
| Keywords | Aminopeptidase;Chloroplast;Hydrolase;Manganese;Metal-binding;Metalloprotease;Plastid;Protease;Reference proteome;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15299147; 16207701; 18431481; 18633119; 28627464; |
| Motif | |
| Gene Encoded By | |
| Mass | 78,709 |
| Kinetics | |
| Metal Binding | METAL 506; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:Q9NQW7; METAL 517; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:Q9NQW7; METAL 517; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:Q9NQW7; METAL 580; /note=Manganese 2; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:Q9NQW7; METAL 614; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:Q9NQW7; METAL 628; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:Q9NQW7; METAL 628; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:Q9NQW7 |
| Rhea ID | |
| Cross Reference Brenda |