Detail Information for IndEnz0002018796
IED ID IndEnz0002018796
Enzyme Type ID protease018796
Protein Name Aminopeptidase P2
AtAPP2
EC 3.4.11.9
Gene Name APP2 At3g05350 T12H1.32
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MIPLTLSSPSLNRLVLSTSRYSHSLFLSNFNSLSLIHRKLPYKPLFGARCHASSSSSSSSSFTAKSSKEIRKAQTKVVVDEKLSSIRRLFSEPGVGIDAYIIPSQDAHQSEFIAECYARRAYISGFTGSAGTAVVTKDKAALWTDGRYFLQAEKQLNSSWILMRAGNPGVPTASEWIADVLAPGGRVGIDPFLFSADAAEELKEVIAKKNHELVYLYNVNLVDEIWKDSRPKPPSRQIRIHDLKYAGLDVASKLLSLRNQIMDAGTSAIVISMLDEIAWVLNLRGSDVPHSPVMYAYLIVEVDQAQLFVDNSKVTVEVKDHLKNAGIELRPYDSILQGIDSLAARGAQLLMDPSTLNVAIISTYKSACERYSRNFESEAKVKTKFTDSSSGYTANPSGIYMQSPISWAKAIKNDAELKGMKNSHLRDAAALAHFWAWLEEEVHKNANLTEVDVADRLLEFRSMQDGFMDTSFDTISGSGANGAIIHYKPEPESCSRVDPQKLFLLDSGAQYVDGTTDITRTVHFSEPSAREKECFTRVLQGHIALDQAVFPEGTPGFVLDGFARSSLWKIGLDYRHGTGHGVGAALNVHEGPQSISFRYGNMTPLQNGMIVSNEPGYYEDHAFGIRIENLLHVRDAETPNRFGGATYLGFEKLTFFPIQTKMVDVSLLSDTEVDWLNSYHAEVWEKVSPLLEGSTTQQWLWNNTRPLAKP
Enzyme Length 710
Uniprot Accession Number Q8RY11
Absorption
Active Site
Activity Regulation
Binding Site BINDING 147; /note=Substrate; /evidence=ECO:0000250|UniProtKB:O44750; BINDING 486; /note=Substrate; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:O44750; BINDING 580; /note=Substrate; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:O44750; BINDING 589; /note=Substrate; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:O44750; BINDING 614; /note=Substrate; /evidence=ECO:0000250|UniProtKB:O44750
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.; EC=3.4.11.9; Evidence={ECO:0000250|UniProtKB:F4JQH3};
DNA Binding
EC Number 3.4.11.9
Enzyme Function FUNCTION: Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro. {ECO:0000250|UniProtKB:Q9VJG0}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Binding site (5); Chain (1); Erroneous gene model prediction (1); Metal binding (7); Transit peptide (1)
Keywords Aminopeptidase;Chloroplast;Hydrolase;Manganese;Metal-binding;Metalloprotease;Plastid;Protease;Reference proteome;Transit peptide
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 15299147; 16207701; 18431481; 18633119; 28627464;
Motif
Gene Encoded By
Mass 78,709
Kinetics
Metal Binding METAL 506; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:Q9NQW7; METAL 517; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:Q9NQW7; METAL 517; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:Q9NQW7; METAL 580; /note=Manganese 2; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:Q9NQW7; METAL 614; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:Q9NQW7; METAL 628; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:Q9NQW7; METAL 628; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:Q9NQW7
Rhea ID
Cross Reference Brenda