| IED ID | IndEnz0002018801 |
| Enzyme Type ID | protease018801 |
| Protein Name |
Astacin-like metalloendopeptidase EC 3.4.24.- Chorioallantoic membrane-1 CAM-1 Quail hatching enzyme QHE |
| Gene Name | |
| Organism | Coturnix japonica (Japanese quail) (Coturnix coturnix japonica) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Archelosauria Archosauria Dinosauria Saurischia Theropoda Coelurosauria Aves Neognathae Galloanserae Galliformes Phasianidae (turkeys) Perdicinae Coturnix Coturnix japonica (Japanese quail) (Coturnix coturnix japonica) |
| Enzyme Sequence | MDLKMLLIFTAFLLPAVLGFPIQDNYENSTATSESTQVTTEESIYDSPSPTETDSEDDVIFNRILEVNKDSSRYLQEGDIVPRRSRSAFNCRNCYWPQSMDGIVRIPYVLDPTYEENHVRGILEAMAEFETLTCINFVKRKTERDYLIIRSADGCWSNYGKVGGGQTVSVMKGGCMWKGIIQHELDHALGFLHEHSRSDRDKYVKIMWEYISPACRPDFRKFENSNNLGLPYDYSSVMHYGPHTFTNTTGKATIVPVPDGSVHIGQRLGLSNLDVAKINKLYNCSRCSTIIDAAFGSLKSANYPRNYSDNTNCVWLIRTRSRKISLHFRDFDLRRTRGCQGDYVKVYDGSSKYSPVLMNKTCGSQIPTDVVSSSSLMLIEFVTDGRDTASGFQATFTSARMQRRFNTRN |
| Enzyme Length | 409 |
| Uniprot Accession Number | P42662 |
| Absorption | |
| Active Site | ACT_SITE 184; /evidence=ECO:0000255|PROSITE-ProRule:PRU01211 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Probable oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization (By similarity). Protease which may play a role in the breaking down of the vitelline membrane (days 0-5) and possibly, in the digestion of the egg white (days 9-12) (PubMed:16003522). {ECO:0000250|UniProtKB:Q6HA09, ECO:0000269|PubMed:16003522}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Alternative sequence (1); Chain (1); Compositional bias (1); Disulfide bond (5); Domain (2); Metal binding (3); Propeptide (1); Region (1); Sequence conflict (3); Signal peptide (1) |
| Keywords | Alternative splicing;Cell membrane;Cytoplasm;Cytoplasmic vesicle;Disulfide bond;Fertilization;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | INDUCTION: By 1,25-dihydroxyvitamin D-3. {ECO:0000269|PubMed:8286408}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q6HA09}. Cell membrane {ECO:0000250|UniProtKB:Q6HA09}. Cytoplasmic vesicle, secretory vesicle, Cortical granule {ECO:0000250|UniProtKB:Q6HA09}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 46,446 |
| Kinetics | |
| Metal Binding | METAL 183; /note=Zinc; via tele nitrogen; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01211; METAL 187; /note=Zinc; via tele nitrogen; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01211; METAL 193; /note=Zinc; via tele nitrogen; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01211 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.21; |