Detail Information for IndEnz0002018801
IED ID IndEnz0002018801
Enzyme Type ID protease018801
Protein Name Astacin-like metalloendopeptidase
EC 3.4.24.-
Chorioallantoic membrane-1
CAM-1
Quail hatching enzyme
QHE
Gene Name
Organism Coturnix japonica (Japanese quail) (Coturnix coturnix japonica)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Archelosauria Archosauria Dinosauria Saurischia Theropoda Coelurosauria Aves Neognathae Galloanserae Galliformes Phasianidae (turkeys) Perdicinae Coturnix Coturnix japonica (Japanese quail) (Coturnix coturnix japonica)
Enzyme Sequence MDLKMLLIFTAFLLPAVLGFPIQDNYENSTATSESTQVTTEESIYDSPSPTETDSEDDVIFNRILEVNKDSSRYLQEGDIVPRRSRSAFNCRNCYWPQSMDGIVRIPYVLDPTYEENHVRGILEAMAEFETLTCINFVKRKTERDYLIIRSADGCWSNYGKVGGGQTVSVMKGGCMWKGIIQHELDHALGFLHEHSRSDRDKYVKIMWEYISPACRPDFRKFENSNNLGLPYDYSSVMHYGPHTFTNTTGKATIVPVPDGSVHIGQRLGLSNLDVAKINKLYNCSRCSTIIDAAFGSLKSANYPRNYSDNTNCVWLIRTRSRKISLHFRDFDLRRTRGCQGDYVKVYDGSSKYSPVLMNKTCGSQIPTDVVSSSSLMLIEFVTDGRDTASGFQATFTSARMQRRFNTRN
Enzyme Length 409
Uniprot Accession Number P42662
Absorption
Active Site ACT_SITE 184; /evidence=ECO:0000255|PROSITE-ProRule:PRU01211
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.24.-
Enzyme Function FUNCTION: Probable oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization (By similarity). Protease which may play a role in the breaking down of the vitelline membrane (days 0-5) and possibly, in the digestion of the egg white (days 9-12) (PubMed:16003522). {ECO:0000250|UniProtKB:Q6HA09, ECO:0000269|PubMed:16003522}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Alternative sequence (1); Chain (1); Compositional bias (1); Disulfide bond (5); Domain (2); Metal binding (3); Propeptide (1); Region (1); Sequence conflict (3); Signal peptide (1)
Keywords Alternative splicing;Cell membrane;Cytoplasm;Cytoplasmic vesicle;Disulfide bond;Fertilization;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Signal;Zinc;Zymogen
Interact With
Induction INDUCTION: By 1,25-dihydroxyvitamin D-3. {ECO:0000269|PubMed:8286408}.
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q6HA09}. Cell membrane {ECO:0000250|UniProtKB:Q6HA09}. Cytoplasmic vesicle, secretory vesicle, Cortical granule {ECO:0000250|UniProtKB:Q6HA09}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..19; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 46,446
Kinetics
Metal Binding METAL 183; /note=Zinc; via tele nitrogen; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01211; METAL 187; /note=Zinc; via tele nitrogen; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01211; METAL 193; /note=Zinc; via tele nitrogen; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01211
Rhea ID
Cross Reference Brenda 3.4.24.21;