Detail Information for IndEnz0002018823
IED ID IndEnz0002018823
Enzyme Type ID protease018823
Protein Name Chymotrypsin
EC 3.4.21.1
Gene Name
Organism Phaedon cochleariae (Mustard beetle)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Coleoptera Polyphaga Cucujiformia Chrysomeloidea Chrysomelidae (leaf beetles) Chrysomelinae Chrysomelini Phaedon Phaedon cochleariae (Mustard beetle)
Enzyme Sequence MKVALVVLALFGVSLAASIDNIEIPPSKNIYVEPINQPEVDPSLEIVNGQEVVPHSIPYQIFLVASAGETSWTCGGSLITKRYVLTAAHCIQGAKSVHVTLGAHNLAKHEASKVTVNGRSWVIHEKYDSTNIDNDIGVIQLERNLTLTRSIQLARLPSLRDVGINLEGRTATVSGWGLTNGIFQTTTDVLRANNTIISNKECNDVFKIVQPTEVCLSIAGGRSACSGDSGGPLVIDNVQHGIVSYGSSYCRSTPSVFTRVSSYLNWLQTHSEWRAQ
Enzyme Length 276
Uniprot Accession Number O97398
Absorption
Active Site ACT_SITE 89; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P00771; ACT_SITE 135; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P00771; ACT_SITE 229; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P00771
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.; EC=3.4.21.1; Evidence={ECO:0000250|UniProtKB:Q00871, ECO:0000255|PROSITE-ProRule:PRU10078, ECO:0000255|PROSITE-ProRule:PRU10079};
DNA Binding
EC Number 3.4.21.1
Enzyme Function FUNCTION: Serine protease with chymotryptic and collagenolytic activities. {ECO:0000250|UniProtKB:Q00871}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (3); Domain (1); Glycosylation (2); Propeptide (1); Signal peptide (1)
Keywords Collagen degradation;Digestion;Disulfide bond;Glycoprotein;Hydrolase;Protease;Secreted;Serine protease;Signal;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, extracellular space {ECO:0000250|UniProtKB:Q00871}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..16; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 29,868
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda