| IED ID | IndEnz0002018823 |
| Enzyme Type ID | protease018823 |
| Protein Name |
Chymotrypsin EC 3.4.21.1 |
| Gene Name | |
| Organism | Phaedon cochleariae (Mustard beetle) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Coleoptera Polyphaga Cucujiformia Chrysomeloidea Chrysomelidae (leaf beetles) Chrysomelinae Chrysomelini Phaedon Phaedon cochleariae (Mustard beetle) |
| Enzyme Sequence | MKVALVVLALFGVSLAASIDNIEIPPSKNIYVEPINQPEVDPSLEIVNGQEVVPHSIPYQIFLVASAGETSWTCGGSLITKRYVLTAAHCIQGAKSVHVTLGAHNLAKHEASKVTVNGRSWVIHEKYDSTNIDNDIGVIQLERNLTLTRSIQLARLPSLRDVGINLEGRTATVSGWGLTNGIFQTTTDVLRANNTIISNKECNDVFKIVQPTEVCLSIAGGRSACSGDSGGPLVIDNVQHGIVSYGSSYCRSTPSVFTRVSSYLNWLQTHSEWRAQ |
| Enzyme Length | 276 |
| Uniprot Accession Number | O97398 |
| Absorption | |
| Active Site | ACT_SITE 89; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P00771; ACT_SITE 135; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P00771; ACT_SITE 229; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P00771 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.; EC=3.4.21.1; Evidence={ECO:0000250|UniProtKB:Q00871, ECO:0000255|PROSITE-ProRule:PRU10078, ECO:0000255|PROSITE-ProRule:PRU10079}; |
| DNA Binding | |
| EC Number | 3.4.21.1 |
| Enzyme Function | FUNCTION: Serine protease with chymotryptic and collagenolytic activities. {ECO:0000250|UniProtKB:Q00871}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3); Domain (1); Glycosylation (2); Propeptide (1); Signal peptide (1) |
| Keywords | Collagen degradation;Digestion;Disulfide bond;Glycoprotein;Hydrolase;Protease;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space {ECO:0000250|UniProtKB:Q00871}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 29,868 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |