Detail Information for IndEnz0002018835
IED ID IndEnz0002018835
Enzyme Type ID protease018835
Protein Name Carboxypeptidase A1
EC 3.4.17.1
Gene Name CPA1 CPA
Organism Bos taurus (Bovine)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Ruminantia Pecora Bovidae Bovinae Bos (oxen cattle) Bos taurus (Bovine)
Enzyme Sequence MQGLLILSVLLGAALGKEDFVGHQVLRITAADEAEVQTVKELEDLEHLQLDFWRGPGQPGSPIDVRVPFPSLQAVKVFLEAHGIRYRIMIEDVQSLLDEEQEQMFASQSRARSTNTFNYATYHTLDEIYDFMDLLVAEHPQLVSKLQIGRSYEGRPIYVLKFSTGGSNRPAIWIDLGIHSREWITQATGVWFAKKFTEDYGQDPSFTAILDSMDIFLEIVTNPDGFAFTHSQNRLWRKTRSVTSSSLCVGVDANRNWDAGFGKAGASSSPCSETYHGKYANSEVEVKSIVDFVKDHGNFKAFLSIHSYSQLLLYPYGYTTQSIPDKTELNQVAKSAVEALKSLYGTSYKYGSIITTIYQASGGSIDWSYNQGIKYSFTFELRDTGRYGFLLPASQIIPTAQETWLGVLTIMEHTLNNLY
Enzyme Length 419
Uniprot Accession Number P00730
Absorption
Active Site ACT_SITE 380; /note=Proton donor/acceptor; /evidence=ECO:0000250|UniProtKB:P00732
Activity Regulation
Binding Site BINDING 237; /note="Substrate"; /evidence="ECO:0000269|PubMed:12431056, ECO:0007744|PDB:1IY7"; BINDING 358; /note="Substrate"; /evidence="ECO:0000269|PubMed:12431056, ECO:0007744|PDB:1IY7"
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.; EC=3.4.17.1; Evidence={ECO:0000250|UniProtKB:P15085}; CATALYTIC ACTIVITY: Reaction=H2O + leukotriene C4 = glycine + leukotriene F4; Xref=Rhea:RHEA:50740, ChEBI:CHEBI:15377, ChEBI:CHEBI:57305, ChEBI:CHEBI:57973, ChEBI:CHEBI:133618; Evidence={ECO:0000269|PubMed:12729612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50741; Evidence={ECO:0000269|PubMed:12729612};
DNA Binding
EC Number 3.4.17.1
Enzyme Function FUNCTION: Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro (By similarity). Catalyzes the conversion of leukotriene C4 to leukotriene F4 via the hydrolysis of an amide bond (PubMed:12729612). {ECO:0000250|UniProtKB:P15085, ECO:0000269|PubMed:12729612}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Beta strand (19); Binding site (2); Chain (1); Disulfide bond (1); Helix (16); Metal binding (3); Natural variant (3); Propeptide (1); Region (3); Sequence conflict (6); Signal peptide (1); Turn (5)
Keywords 3D-structure;Carboxypeptidase;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P15085}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..16; /evidence=ECO:0000269|PubMed:3147705
Structure 3D X-ray crystallography (32)
Cross Reference PDB 1ARL; 1ARM; 1BAV; 1CBX; 1CPS; 1CPX; 1EE3; 1ELL; 1ELM; 1F57; 1HDQ; 1HDU; 1HEE; 1IY7; 1M4L; 1PYT; 1YME; 1ZLH; 2ABZ; 2CTB; 2CTC; 2RFH; 3CPA; 3FVL; 3FX6; 3I1U; 3KGQ; 4CPA; 5CPA; 6CPA; 7CPA; 8CPA;
Mapped Pubmed ID 10742178; 11941507; 1527041; 15561703; 15961103; 16084391; 16126224; 1738164; 18289863; 1868092; 19646864; 19895506; 21804935; 2386784; 3463986; 7154070; 9843422;
Motif
Gene Encoded By
Mass 47,082
Kinetics
Metal Binding METAL 179; /note="Zinc; catalytic"; /evidence="ECO:0000269|PubMed:12431056, ECO:0007744|PDB:1IY7"; METAL 182; /note="Zinc; catalytic"; /evidence="ECO:0000269|PubMed:12431056, ECO:0007744|PDB:1IY7"; METAL 306; /note="Zinc; catalytic"; /evidence="ECO:0000269|PubMed:12431056, ECO:0007744|PDB:1IY7"
Rhea ID RHEA:50740; RHEA:50741
Cross Reference Brenda 3.4.17.1;