| IED ID | IndEnz0002018844 |
| Enzyme Type ID | protease018844 |
| Protein Name |
Vacuolar protease A EC 3.4.23.25 Aspartic endopeptidase PEP2 Aspartic protease PEP2 |
| Gene Name | PEP2 MCYG_05527 |
| Organism | Arthroderma otae (strain ATCC MYA-4605 / CBS 113480) (Microsporum canis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Onygenales Arthrodermataceae (dermatophytes) Microsporum Arthroderma otae (Microsporum canis) Arthroderma otae (strain ATCC MYA-4605 / CBS 113480) (Microsporum canis) |
| Enzyme Sequence | MKGSLLLAGATLLGCTSAKLHSLKLKKVSLKEQLEHADIDVQIKSLGQKYMGIRPGQHEQQMFKEQTPIEAESGHNVLIDNFLNAQYFSEISIGTPPQTFKVVLDTGSSNLWVPGKDCSSIACFLHSTYDSSASSTFTRNGTSFAIRYGSGSLEGFVSQDNVQIGDMKIKNQLFAEATSEPGLAFAFGRFDGILGMGYDTISVNKITPPFYKMVEQGLVDEPVFSFYLGDTNKDGDQSVVTFGGADKSHYTGDITTIPLRRKAYWEVEFNAITLGKDTATLDNTGIILDTGTSLIALPTTYAEMIISKSWNGQYTIDCAKRDSLPDLTFTLSGHNFTIGPYDYTLEVSGTCISSFMGMDFPEPVGPLAILGDSFLRRWYSVYDLGKGTVGLAKAK |
| Enzyme Length | 395 |
| Uniprot Accession Number | C5FS55 |
| Absorption | |
| Active Site | ACT_SITE 105; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094; ACT_SITE 289; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins with broad specificity for peptide bonds. Cleaves -Leu-Leu-|-Val-Tyr- bond in a synthetic substrate. Does not act on esters of Tyr or Arg.; EC=3.4.23.25; |
| DNA Binding | |
| EC Number | 3.4.23.25 |
| Enzyme Function | FUNCTION: Vacuolar aspartic endopeptidase which is probably also secreted and contributes to virulence. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (2); Domain (1); Glycosylation (2); Propeptide (1); Signal peptide (1) |
| Keywords | Aspartyl protease;Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Signal;Vacuole;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Vacuole lumen. Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 43,077 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |