Detail Information for IndEnz0002018847
IED ID IndEnz0002018847
Enzyme Type ID protease018847
Protein Name Endothiapepsin
EC 3.4.23.22
Aspartate protease
Gene Name EAPA EPN-1
Organism Cryphonectria parasitica (Chestnut blight fungus) (Endothia parasitica)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Diaporthales Cryphonectriaceae Cryphonectria-Endothia species complex Cryphonectria Cryphonectria parasitica (Chestnut blight fungus) (Endothia parasitica)
Enzyme Sequence MSSPLKNALVTAMLAGGALSSPTKQHVGIPVNASPEVGPGKYSFKQVRNPNYKFNGPLSVKKTYLKYGVPIPAWLEDAVQNSTSGLAERSTGSATTTPIDSLDDAYITPVQIGTPAQTLNLDFDTGSSDLWVFSSETTASEVDGQTIYTPSKSTTAKLLSGATWSISYGDGSSSSGDVYTDTVSVGGLTVTGQAVESAKKVSSSFTEDSTIDGLLGLAFSTLNTVSPTQQKTFFDNAKASLDSPVFTADLGYHAPGTYNFGFIDTTAYTGSITYTAVSTKQGFWEWTSTGYAVGSGTFKSTSIDGIADTGTTLLYLPATVVSAYWAQVSGAKSSSSVGGYVFPCSATLPSFTFGVGSARIVIPGDYIDFGPISTGSSSCFGGIQSSAGIGINIFGDVALKAAFVVFNGATTPTLGFASK
Enzyme Length 419
Uniprot Accession Number P11838
Absorption
Active Site ACT_SITE 124; ACT_SITE 288
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins with specificity similar to that of pepsin A, prefers hydrophobic residues at P1 and P1', but does not cleave 14-Ala-|-Leu-15 in the B chain of insulin or Z-Glu-Tyr. Clots milk.; EC=3.4.23.22;
DNA Binding
EC Number 3.4.23.22
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Beta strand (29); Chain (1); Disulfide bond (1); Domain (1); Helix (8); Propeptide (1); Signal peptide (1); Turn (4)
Keywords 3D-structure;Aspartyl protease;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Signal;Zymogen
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..20; /evidence=ECO:0000255
Structure 3D Neutron diffraction (2); X-ray crystallography (739)
Cross Reference PDB 1E5O; 1E80; 1E81; 1E82; 1EED; 1ENT; 1EPL; 1EPM; 1EPN; 1EPO; 1EPP; 1EPQ; 1EPR; 1ER8; 1GKT; 1GVT; 1GVU; 1GVV; 1GVW; 1GVX; 1OD1; 1OEW; 1OEX; 2ER0; 2ER6; 2ER7; 2ER9; 2JJI; 2JJJ; 2V00; 2VS2; 3ER3; 3ER5; 3LZY; 3PB5; 3PBD; 3PBZ; 3PCW; 3PCZ; 3PGI; 3PI0; 3PLD; 3PLL; 3PM4; 3PMU; 3PMY; 3PRS; 3PSY; 3PWW; 3Q6Y; 3T6I; 3T7P; 3T7Q; 3T7X; 3URI; 3URJ; 3URL; 3WZ6; 3WZ7; 3WZ8; 4APE; 4ER1; 4ER2; 4ER4; 4KUP; 4L6B; 4LAP; 4LBT; 4LHH; 4LP9; 4Y35; 4Y36; 4Y37; 4Y38; 4Y39; 4Y3A; 4Y3D; 4Y3E; 4Y3F; 4Y3G; 4Y3H; 4Y3J; 4Y3L; 4Y3M; 4Y3N; 4Y3P; 4Y3Q; 4Y3R; 4Y3S; 4Y3T; 4Y3W; 4Y3X; 4Y3Y; 4Y3Z; 4Y41; 4Y43; 4Y44; 4Y45; 4Y47; 4Y48; 4Y4A; 4Y4B; 4Y4D; 4Y4E; 4Y4G; 4Y4J; 4Y4T; 4Y4U; 4Y4W; 4Y4X; 4Y4Z; 4Y50; 4Y51; 4Y53; 4Y54; 4Y56; 4Y57; 4Y58; 4Y5A; 4Y5B; 4Y5C; 4Y5E; 4Y5G; 4Y5K; 4Y5L; 4Y5M; 4Y5N; 4Y5P; 4YCK; 4YCT; 4YCY; 4YD3; 4YD4; 4YD5; 4YD6; 4YD7; 4ZE6; 4ZEA; 5DPZ; 5DQ1; 5DQ2; 5DQ4; 5DQ5; 5DR0; 5DR1; 5DR3; 5DR4; 5DR7; 5DR8; 5ER1; 5ER2; 5HCO; 5HCT; 5IS4; 5ISJ; 5ISK; 5J25; 5LWR; 5LWS; 5LWT; 5LWU; 5MB0; 5MB3; 5MB5; 5MB6; 5MB7; 5OG7; 5OJE; 5OYQ; 5OYR; 5OYS; 5OYT; 5OYU; 5OYV; 5OYW; 5OYX; 5OYY; 5OYZ; 5OZ0; 5OZ1; 5OZ2; 5OZ3; 5OZ4; 5OZ5; 5OZ6; 5OZ7; 5OZ8; 5OZ9; 5OZA; 5OZB; 5OZC; 5OZD; 5OZE; 5OZF; 5OZG; 5OZH; 5OZI; 5OZJ; 5OZK; 5OZL; 5OZM; 5OZN; 5OZO; 5OZP; 5OZQ; 5OZR; 5OZS; 5OZT; 5OZU; 5OZV; 5OZW; 5OZX; 5OZY; 5OZZ; 5P00; 5P01; 5P02; 5P03; 5P04; 5P05; 5P06; 5P07; 5P08; 5P09; 5P0A; 5P0B; 5P0C; 5P0D; 5P0E; 5P0F; 5P0G; 5P0H; 5P0I; 5P0J; 5P0K; 5P0L; 5P0M; 5P0N; 5P0O; 5P0P; 5P0Q; 5P0R; 5P0S; 5P0T; 5P0U; 5P0V; 5P0W; 5P0X; 5P0Y; 5P0Z; 5P10; 5P11; 5P12; 5P13; 5P14; 5P15; 5P16; 5P17; 5P18; 5P19; 5P1A; 5P1B; 5P1C; 5P1D; 5P1E; 5P1F; 5P1G; 5P1H; 5P1I; 5P1J; 5P1K; 5P1L; 5P1M; 5P1N; 5P1O; 5P1P; 5P1Q; 5P1R; 5P1S; 5P1T; 5P1U; 5P1V; 5P1W; 5P1X; 5P1Y; 5P1Z; 5P20; 5P22; 5P23; 5P24; 5P25; 5P26; 5P27; 5P28; 5P29; 5P2A; 5P2B; 5P2C; 5P2D; 5P2E; 5P2F; 5P2G; 5P2H; 5P2I; 5P2J; 5P2K; 5P2L; 5P2M; 5P2N; 5P2O; 5P2Q; 5P2R; 5P2S; 5P2T; 5P2U; 5P2V; 5P2W; 5P2X; 5P2Y; 5P2Z; 5P30; 5P31; 5P32; 5P33; 5P34; 5P35; 5P36; 5P37; 5P38; 5P39; 5P3A; 5P3B; 5P3C; 5P3D; 5P3E; 5P3F; 5P3G; 5P3H; 5P3I; 5P3J; 5P3K; 5P3L; 5P3M; 5P3N; 5P3O; 5P3P; 5P3Q; 5P3R; 5P3S; 5P3T; 5P3U; 5P3V; 5P3W; 5P3X; 5P3Y; 5P3Z; 5P40; 5P41; 5P42; 5P43; 5P44; 5P45; 5P46; 5P47; 5P48; 5P49; 5P4A; 5P4B; 5P4C; 5P4D; 5P4E; 5P4F; 5P4G; 5P4H; 5P4I; 5P4J; 5P4K; 5P4L; 5P4M; 5P4N; 5P4O; 5P4P; 5P4Q; 5P4R; 5P4S; 5P4T; 5P4U; 5P4V; 5P4W; 5P4X; 5P4Y; 5P4Z; 5P50; 5P51; 5P52; 5P53; 5P54; 5P55; 5P56; 5P57; 5P58; 5P59; 5P5A; 5P5B; 5P5C; 5P5D; 5P5E; 5P5F; 5P5G; 5P5H; 5P5I; 5P5J; 5P5K; 5P5L; 5P5M; 5P5N; 5P5O; 5P5P; 5P5Q; 5P5R; 5P5S; 5P5T; 5P5U; 5P5V; 5P5W; 5P5X; 5P5Y; 5P5Z; 5P60; 5P61; 5P62; 5P63; 5P64; 5P65; 5P66; 5P67; 5P68; 5P69; 5P6A; 5P6B; 5P6C; 5P6D; 5P6E; 5P6F; 5P6G; 5P6H; 5P6I; 5P6J; 5P6K; 5P6L; 5P6M; 5P6N; 5P6O; 5P6P; 5P6Q; 5P6R; 5P6S; 5P6T; 5P6U; 5P6V; 5P6W; 5P6X; 5P6Y; 5P6Z; 5P70; 5P71; 5P72; 5P73; 5P74; 5P75; 5P76; 5P77; 5P78; 5P79; 5P7A; 5P7B; 5P7C; 5P7D; 5P7E; 5P7F; 5P7G; 5P7H; 5P7I; 5P7J; 5P7K; 5P7L; 5P7M; 5P7N; 5P7O; 5P7P; 5P7Q; 5P7R; 5P7S; 5P7T; 5P7U; 5P7V; 5P7W; 5P7X; 5P7Y; 5P7Z; 5P80; 5P81; 5P82; 5P83; 5P84; 5P85; 5P86; 5P87; 5P88; 5P89; 5P8A; 5P8B; 5P8C; 5P8D; 5P8E; 5P8F; 5P8G; 5P8H; 5P8I; 5P8J; 5P8K; 5P8L; 5P8M; 5P8N; 5P8O; 5P8P; 5P8Q; 5P8R; 5P8S; 5P8T; 5P8U; 5P8V; 5QB5; 5QB6; 5QB7; 5QB8; 5QB9; 5QBA; 5QBB; 5QBC; 5QBD; 5QBE; 5QBF; 5QBG; 5QBH; 5QBI; 5QBJ; 5QBK; 5QBL; 5QBM; 5QBN; 5QBO; 5QBP; 5QBQ; 5QBR; 5QBS; 5QBT; 5R1T; 5R1U; 5R1V; 5R1W; 5R1X; 5R1Y; 5R1Z; 5R20; 5R21; 5R22; 5R23; 5R24; 5R25; 5R26; 5R27; 5R28; 5R29; 5R2A; 5R2B; 5R2C; 5R2D; 5R2E; 5R2F; 5R2G; 5R2H; 5R2I; 5R2J; 5R2K; 5R2L; 5R2M; 5R2N; 5R2O; 5R2P; 5R2Q; 5R2R; 5R2S; 5R2T; 5R2U; 5R2V; 5R2W; 5R2X; 5R2Y; 5R2Z; 5R30; 5R31; 5R32; 5R33; 5R34; 5R35; 5R36; 5R37; 5R38; 5R39; 5R3A; 5R3B; 5R3C; 5R3D; 5R3E; 5R3F; 5R3G; 5R3H; 5R3I; 5R3J; 5R3K; 5R3L; 5R3M; 5R3N; 5R3O; 5R3P; 5R3Q; 5R3R; 5R3S; 5R3T; 5R3U; 5R3V; 5R3W; 5R3X; 5R3Y; 5R3Z; 5R40; 5R41; 5RBO; 5RBP; 5RBQ; 5RBR; 5RBS; 5RBT; 5RBU; 5RBV; 5RBW; 5RBX; 5RBY; 5RBZ; 5RC0; 5RC1; 5RC2; 5RC3; 5RC4; 5RC5; 5RC6; 5RC7; 5RC8; 5RC9; 5RCA; 5RCB; 5RCC; 5RCD; 5RCE; 5RCF; 5RCG; 5RCH; 5RCI; 5RCJ; 5RCK; 5RCL; 5RCM; 5RCN; 5RCO; 5RCP; 5RCQ; 5RCR; 5RCS; 5RCT; 5RCU; 5RCV; 5RCW; 5RCX; 5RCY; 5RCZ; 5RD0; 5RD1; 5RD2; 5RD3; 5RD4; 5RD5; 5RD6; 5RD7; 5RD8; 5RD9; 5RDA; 5RDB; 5RDC; 5RDD; 5RDE; 5RDF; 5RDG; 5RDH; 5RDI; 5RDJ; 5RDK; 5RDL; 5RDM; 5RDN; 5RDO; 5RDP; 5RDQ; 5RDR; 5RDS; 5RDT; 5RDU; 5RDV; 5RDW; 5RDX; 5RDY; 5RDZ; 5RE0; 5RE1; 5RE2; 5RE3; 5SAK; 5SAL; 5SAM; 5SAN; 5SAO; 5SAP; 5SAQ; 5SAR; 5SAS; 5SAT; 6RON; 6RSV; 6SCV; 6ZZ2; 7ADG;
Mapped Pubmed ID 11683623; 12777758; 12876323; 1304340; 1525155; 17985861; 18084100; 18479128; 21972967; 22213702; 22525752; 2266553; 24419612; 24532096; 25630461; 2690945; 27139825; 27400756; 27452405; 27463859; 27726357; 28097765; 30178575; 3119339; 32413289; 3295561; 34473087; 6381096; 7703859; 8254610; 8424781;
Motif
Gene Encoded By
Mass 43,255
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda 3.4.23.22;