Detail Information for IndEnz0002018856
IED ID IndEnz0002018856
Enzyme Type ID protease018856
Protein Name Rhizopuspepsin
EC 3.4.23.21
Gene Name
Organism Rhizopus chinensis (Bread mold)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Fungi incertae sedis Mucoromycota Mucoromycotina Mucoromycetes Mucorales (pin molds) Mucorineae Rhizopodaceae Rhizopus Rhizopus microsporus Rhizopus chinensis (Bread mold)
Enzyme Sequence MKFTLISSCIAIAALAVAVDAAPGEKKISIPLAKNPNYKPSAKNAIQKAIAKYNKHKINTSTGGIVPDAGVGTVPMTDYGNDVEYYGQVTIGTPGKKFNLDFDTGSSDLWIASTLCTNCGSRQTKYDPKQSSTYQADGRTWSISYGDGSSASGILAKDNVNLGGLLIKGQTIELAKREAASFANGPNDGLLGLGFDTITTVRGVKTPMDNLISQGLISRPIFGVYLGKASNGGGGEYIFGGYDSTKFKGSLTTVPIDNSRGWWGITVDRATVGTSTVASSFDGILDTGTTLLILPNNVAASVARAYGASDNGDGTYTISCDTSRFKPLVFSINGASFQVSPDSLVFEEYQGQCIAGFGYGNFDFAIIGDTFLKNNYVVFNQGVPEVQIAPVAQ
Enzyme Length 393
Uniprot Accession Number P06026
Absorption
Active Site ACT_SITE 103; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094; ACT_SITE 286; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins with broad specificity similar to that of pepsin A, preferring hydrophobic residues at P1 and P1'. Clots milk and activates trypsinogen. Does not cleave 4-Gln-|-His-5, but does cleave 10-His-|-Leu-11 and 12-Val-|-Glu-13 in B chain of insulin.; EC=3.4.23.21;
DNA Binding
EC Number 3.4.23.21
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Beta strand (25); Chain (1); Disulfide bond (2); Domain (1); Helix (10); Natural variant (8); Propeptide (1); Signal peptide (1); Turn (3)
Keywords 3D-structure;Aspartyl protease;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Signal;Zymogen
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..21; /evidence=ECO:0000255
Structure 3D X-ray crystallography (8)
Cross Reference PDB 1UH7; 1UH8; 1UH9; 2APR; 3APR; 4APR; 5APR; 6APR;
Mapped Pubmed ID 14501114; 1603809; 3313384;
Motif
Gene Encoded By
Mass 41,327
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda