Detail Information for IndEnz0002018860
IED ID IndEnz0002018860
Enzyme Type ID protease018860
Protein Name Capsid protein alpha
Cleaved into: Capsid protein beta
EC 3.4.23.44
Coat protein beta
Nodavirus endopeptidase
; Peptide gamma
Coat protein gamma
Gene Name alpha
Organism Pariacoto virus (PaV)
Taxonomic Lineage Viruses Riboviria Orthornavirae Kitrinoviricota Magsaviricetes Nodamuvirales Nodaviridae Alphanodavirus Pariacoto virus (PaV)
Enzyme Sequence MVSRTKNRRNKARKVVSRSTALVPMAPASQRTGPAPRKPRKRNQALVRNPRLTDAGLAFLKCAFAAPDFSVDPGKGIPDNFHGRTLAIKDCNTTSVVFTPNTDTYIVVAPVPGFAYFRAEVAVGAQPTTFVGVPYPTYATNFGAGSQNGLPAVNNYSKFRYASMACGLYPTSNMMQFSGSVQVWRVDLNLSEAVNPAVTAITPAPGVFANFVDKRINGLRGIRPLAPRDNYSGNFIDGAYTFAFDKSTDFEWCDFVRSLEFSESNVLGAATAMKLLAPGGGTDTTLTGLGNVNTLVYKISTPTGAVNTAILRTWNCIELQPYTDSALFQFSGVSPPFDPLALECYHNLKMRFPVAVSSRENSKFWEGVLRVLNQISGTLSVIPGPVGTISAGVHQLTGMYM
Enzyme Length 401
Uniprot Accession Number Q9J7Z0
Absorption
Active Site ACT_SITE 68; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of an asparaginyl bond involved in the maturation of the structural protein of the virus, typically -Asn-|-Ala- or -Asn-|-Phe-.; EC=3.4.23.44;
DNA Binding
EC Number 3.4.23.44
Enzyme Function FUNCTION: Capsid protein alpha self-assembles to form an icosahedral procapsid with a T=3 symmetry, about 30 nm in diameter, and consisting of 60 capsid proteins trimers. In addition, calcium ions are incorporated per capsid during assembly. The capsid encapsulates the two genomic RNAs. Capsid maturation occurs via autoproteolytic cleavage of capsid protein alpha generating capsid protein beta and the membrane-active peptide gamma (By similarity). {ECO:0000250}.; FUNCTION: [Peptide gamma]: Membrane-permeabilizing peptide produced by virus maturation, thereby creating the infectious virion. After endocytosis into the host cell, peptide gamma is probably exposed in endosomes, where it permeabilizes the endosomal membrane, facilitating translocation of viral capsid or RNA into the cytoplasm (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Beta strand (19); Chain (3); Compositional bias (1); Disulfide bond (1); Helix (8); Metal binding (2); Region (1); Turn (4)
Keywords 3D-structure;Aspartyl protease;Calcium;Capsid protein;Disulfide bond;Hydrolase;Metal-binding;Protease;T=3 icosahedral capsid protein;Viral penetration into host cytoplasm;Viral penetration via permeabilization of host membrane;Virion;Virus entry into host cell
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: [Capsid protein beta]: Virion {ECO:0000305}.; SUBCELLULAR LOCATION: [Peptide gamma]: Virion {ECO:0000305}. Note=located inside the capsid. {ECO:0000305}.
Modified Residue
Post Translational Modification PTM: Capsid protein alpha autocatalytically maturates into capsid protein beta and peptide gamma. {ECO:0000250}.
Signal Peptide
Structure 3D X-ray crystallography (1)
Cross Reference PDB 1F8V;
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 43,326
Kinetics
Metal Binding METAL 249; /note=Calcium; METAL 251; /note=Calcium
Rhea ID
Cross Reference Brenda