| IED ID | IndEnz0002018864 |
| Enzyme Type ID | protease018864 |
| Protein Name |
CD-NTase-associated protein 3 Probable metalloprotease Cap3 |
| Gene Name | cap3 ESG_RS0100125 geneD |
| Organism | Escherichia coli |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli |
| Enzyme Sequence | MNMNELVFIDDFDNHVVIMSEVVMRLNSYRQTHYTSTESGGTLIGERRGQHLVITHISEPGQDDVRNRTGLERKGIHHQQKVNDLFQQSNGFIVYLGEWHTHPEDFPHPSFIDIKSWVMGIVATEPMIMLIVGRKDIWIGKKIKNDIKKLKKKMVS |
| Enzyme Length | 156 |
| Uniprot Accession Number | P0DTF3 |
| Absorption | |
| Active Site | ACT_SITE 38; /note=Proton donor/acceptor; /evidence=ECO:0000250|UniProtKB:D4GTS4 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: CBASS (cyclic oligonucleotide-based antiphage signaling system) provides immunity against bacteriophage. The CD-NTase protein synthesizes cyclic nucleotides in response to infection; these serve as specific second messenger signals. The signals activate a diverse range of effectors, leading to bacterial cell death and thus abortive phage infection. A type II-A(GA) CBASS system (PubMed:32839535). {ECO:0000269|PubMed:31533127, ECO:0000303|PubMed:32839535}.; FUNCTION: Protects E.coli against phage infection. When capV and dncV are introduced in E.coli MG1655 there is 1000-fold protection against phage P1; protection against other phage (T4, T5 and T6) requires the 2 subsequent genes. {ECO:0000269|PubMed:31533127}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (1); Metal binding (3); Mutagenesis (1) |
| Keywords | Antiviral defense;Hydrolase;Metal-binding;Metalloprotease;Protease;Zinc |
| Interact With | |
| Induction | INDUCTION: Part of the CBASS operon consisting of capV-dncV-cap2-cap3. {ECO:0000305|PubMed:31533127}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 18,095 |
| Kinetics | |
| Metal Binding | METAL 100; /note=Zinc; catalytic; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:Q8U1Y4; METAL 102; /note=Zinc; catalytic; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:Q8U1Y4; METAL 113; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:Q8U1Y4 |
| Rhea ID | |
| Cross Reference Brenda |