Detail Information for IndEnz0002018865
IED ID IndEnz0002018865
Enzyme Type ID protease018865
Protein Name Brachyurin
EC 3.4.21.32
Collagenolytic protease
Gene Name
Organism Leptuca pugilator (Atlantic sand fiddler crab) (Uca pugilator)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Crustacea Multicrustacea Malacostraca Eumalacostraca Eucarida Decapoda Pleocyemata Brachyura (short-tailed crabs) Eubrachyura Thoracotremata Ocypodoidea Ocypodidae Gelasiminae Leptuca Leptuca pugilator (Atlantic sand fiddler crab) (Uca pugilator)
Enzyme Sequence IVGGVEAVPNSWPHQAALFIDDMYFCGGSLISPEWILTAAHCMDGAGFVDVVLGAHNIREDEATQVTIQSTDFTVHENYNSFVISNDIAVIRLPVPVTLTAAIATVGLPSTDVGVGTVVTPTGWGLPSDSALGISDVLRQVDVPIMSNADCDAVYGIVTDGNICIDSTGGKGTCNGDSGGPLNYNGLTYGITSFGAAAGCEAGYPDAFTRVTYFLDWIQTQTGITP
Enzyme Length 226
Uniprot Accession Number P00771
Absorption
Active Site ACT_SITE 41; /note=Charge relay system; ACT_SITE 87; /note=Charge relay system; ACT_SITE 178; /note=Charge relay system
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins, with broad specificity for peptide bonds. Native collagen is cleaved about 75% of the length of the molecule from the N-terminus. Low activity on small molecule substrates of both trypsin and chymotrypsin.; EC=3.4.21.32;
DNA Binding
EC Number 3.4.21.32
Enzyme Function FUNCTION: This enzyme is a serine protease capable of degrading the native triple helix of collagen.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Beta strand (16); Chain (1); Disulfide bond (3); Domain (1); Helix (4); Sequence conflict (4); Turn (3)
Keywords 3D-structure;Collagen degradation;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Serine protease
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (1)
Cross Reference PDB 1AZZ;
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 23,511
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda