Detail Information for IndEnz0002018897
IED ID IndEnz0002018897
Enzyme Type ID protease018897
Protein Name Bleomycin hydrolase
BH
BLM hydrolase
BMH
EC 3.4.22.40
Aminopeptidase H
Gene Name BLMH
Organism Gallus gallus (Chicken)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Archelosauria Archosauria Dinosauria Saurischia Theropoda Coelurosauria Aves Neognathae Galloanserae Galliformes Phasianidae (turkeys) Phasianinae Gallus Gallus gallus (Chicken)
Enzyme Sequence MNAHGLSTEKAAAFTRRLRAEPQFLLAQNVATCSDPLEVCLQRQVVQDTIQVFQHAVPAEGKPVTNQKNSGRCWIFSCLNAMRLPFMKKYNIEEFEFSQSYLFFWDKVERCYYFLNAFVETAQKKEPIDGRLVQFLLTNPTNDGGQWDMLVNIVEKYGVVPKKYFPESHTTEATRRMNEILNHKMREYCLRLRNMVATGTNKEELCAAMDTMIEEVFRIVSTCLGNPPETFCWEFRDKEKNYHKFGPMTPVQFYNEHVKPYFNMEDKVCLVNDPRPQNPYCQLYTVEYLGNMAGGRKTLYNNQPIEVLKKLAATSIKDGEAVWFGCDVAKHFYSKLGINDLNIFNHELVFGVSVKNMNKAERLIFGDSLMTHAMVLTAVSEKDGQEDCYEKWRVENSWGEDRGNKGYLIMTDDWFSEYVYEVVVDKKYVPEDVLAVMEQEPIVLPAWDPMGALAK
Enzyme Length 455
Uniprot Accession Number P87362
Absorption
Active Site ACT_SITE 73; /evidence=ECO:0000255|PROSITE-ProRule:PRU10088; ACT_SITE 372; /evidence=ECO:0000255|PROSITE-ProRule:PRU10088; ACT_SITE 396; /evidence=ECO:0000255|PROSITE-ProRule:PRU10088
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.; EC=3.4.22.40;
DNA Binding
EC Number 3.4.22.40
Enzyme Function FUNCTION: The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Protease;Reference proteome;Thiol protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q13867}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 52,690
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda