| IED ID | IndEnz0002018900 |
| Enzyme Type ID | protease018900 |
| Protein Name |
Thermostable carboxypeptidase 1 EC 3.4.17.19 TthCP1 |
| Gene Name | TTHA0270 |
| Organism | Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Deinococcus-Thermus Deinococci Thermales Thermaceae Thermus Thermus thermophilus Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) |
| Enzyme Sequence | MTPEAAYQNLLEFQRETAYLASLGALAAWDQRTMIPKKGHEHRARQMAALARLLHQRMTDPRIGEWLEKVEGSPLVQDPLSDAAVNVREWRQAYERARAIPERLAVELAQAESEAESFWEEARPRDDWRGFLPYLKRVYALTKEKAEVLFALPPAPGDPPYGELYDALLDGYEPGMRARELLPLFAELKEGLKGLLDRILGSGKRPDTSILHRPYPVEAQRRFALELLSACGYDLEAGRLDPTAHPFEIAIGPGDVRITTRYYEDFFNAGIFGTLHEMGHALYEQGLPKEHWGTPRGDAVSLGVHESQSRTWENLVGRSLGFWERFFPRAREVFASLGDVSLEDFHFAVNAVEPSLIRVEADEVTYNLHILVRLELELALFRGELSPEDLPEAWAEKYRDHLGVAPKDYKDGVMQDVHWAGGLFGYFPTYTLGNLYAAQFFQKAEAELGPLEPRFARGEFQPFLDWTRARIHAEGSRFRPRVLVERVTGEAPSARPFLAYLEKKYAALYG |
| Enzyme Length | 510 |
| Uniprot Accession Number | Q5SLM3 |
| Absorption | |
| Active Site | ACT_SITE 277; /note=Proton donor/acceptor; /evidence=ECO:0000250|UniProtKB:P42663 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of a C-terminal amino acid with broad specificity, except for -Pro.; EC=3.4.17.19; Evidence={ECO:0000269|PubMed:19544567}; |
| DNA Binding | |
| EC Number | 3.4.17.19 |
| Enzyme Function | FUNCTION: Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues, but not Pro. Has lower activity with substrates ending with Gly or Glu. {ECO:0000269|PubMed:19544567}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (4); Chain (1); Helix (29); Metal binding (3); Turn (5) |
| Keywords | 3D-structure;Carboxypeptidase;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 3HOA; 5WVU; |
| Mapped Pubmed ID | 28161633; |
| Motif | |
| Gene Encoded By | |
| Mass | 58,014 |
| Kinetics | |
| Metal Binding | METAL 276; /note=Zinc; via tele nitrogen; /evidence=ECO:0000269|PubMed:15272172; METAL 280; /note=Zinc; via tele nitrogen; /evidence=ECO:0000269|PubMed:15272172; METAL 306; /note=Zinc; /evidence=ECO:0000269|PubMed:15272172 |
| Rhea ID | |
| Cross Reference Brenda |