Detail Information for IndEnz0002018900
IED ID IndEnz0002018900
Enzyme Type ID protease018900
Protein Name Thermostable carboxypeptidase 1
EC 3.4.17.19
TthCP1
Gene Name TTHA0270
Organism Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Deinococcus-Thermus Deinococci Thermales Thermaceae Thermus Thermus thermophilus Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
Enzyme Sequence MTPEAAYQNLLEFQRETAYLASLGALAAWDQRTMIPKKGHEHRARQMAALARLLHQRMTDPRIGEWLEKVEGSPLVQDPLSDAAVNVREWRQAYERARAIPERLAVELAQAESEAESFWEEARPRDDWRGFLPYLKRVYALTKEKAEVLFALPPAPGDPPYGELYDALLDGYEPGMRARELLPLFAELKEGLKGLLDRILGSGKRPDTSILHRPYPVEAQRRFALELLSACGYDLEAGRLDPTAHPFEIAIGPGDVRITTRYYEDFFNAGIFGTLHEMGHALYEQGLPKEHWGTPRGDAVSLGVHESQSRTWENLVGRSLGFWERFFPRAREVFASLGDVSLEDFHFAVNAVEPSLIRVEADEVTYNLHILVRLELELALFRGELSPEDLPEAWAEKYRDHLGVAPKDYKDGVMQDVHWAGGLFGYFPTYTLGNLYAAQFFQKAEAELGPLEPRFARGEFQPFLDWTRARIHAEGSRFRPRVLVERVTGEAPSARPFLAYLEKKYAALYG
Enzyme Length 510
Uniprot Accession Number Q5SLM3
Absorption
Active Site ACT_SITE 277; /note=Proton donor/acceptor; /evidence=ECO:0000250|UniProtKB:P42663
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of a C-terminal amino acid with broad specificity, except for -Pro.; EC=3.4.17.19; Evidence={ECO:0000269|PubMed:19544567};
DNA Binding
EC Number 3.4.17.19
Enzyme Function FUNCTION: Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues, but not Pro. Has lower activity with substrates ending with Gly or Glu. {ECO:0000269|PubMed:19544567}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Beta strand (4); Chain (1); Helix (29); Metal binding (3); Turn (5)
Keywords 3D-structure;Carboxypeptidase;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (2)
Cross Reference PDB 3HOA; 5WVU;
Mapped Pubmed ID 28161633;
Motif
Gene Encoded By
Mass 58,014
Kinetics
Metal Binding METAL 276; /note=Zinc; via tele nitrogen; /evidence=ECO:0000269|PubMed:15272172; METAL 280; /note=Zinc; via tele nitrogen; /evidence=ECO:0000269|PubMed:15272172; METAL 306; /note=Zinc; /evidence=ECO:0000269|PubMed:15272172
Rhea ID
Cross Reference Brenda