Detail Information for IndEnz0002018917
IED ID IndEnz0002018917
Enzyme Type ID protease018917
Protein Name Cathepsin B
EC 3.4.22.1
APP secretase
APPS
Cathepsin B1

Cleaved into: Cathepsin B light chain; Cathepsin B heavy chain
Gene Name CTSB CPSB
Organism Homo sapiens (Human)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human)
Enzyme Sequence MWQLWASLCCLLVLANARSRPSFHPLSDELVNYVNKRNTTWQAGHNFYNVDMSYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQYWEKI
Enzyme Length 339
Uniprot Accession Number P07858
Absorption
Active Site ACT_SITE 108; /evidence=ECO:0000269|PubMed:9299326; ACT_SITE 278; /evidence=ECO:0000269|PubMed:9299326; ACT_SITE 298; /evidence=ECO:0000269|PubMed:9299326
Activity Regulation ACTIVITY REGULATION: Inhibited by leupeptin. {ECO:0000269|PubMed:12220505}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.; EC=3.4.22.1; Evidence={ECO:0000269|PubMed:12220505, ECO:0000269|PubMed:3972105};
DNA Binding
EC Number 3.4.22.1
Enzyme Function FUNCTION: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed:12220505). Cleaves matrix extracellular phosphoglycoprotein MEPE (PubMed:12220505). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis (PubMed:3972105). {ECO:0000250|UniProtKB:P10605, ECO:0000269|PubMed:12220505, ECO:0000269|PubMed:3972105}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Beta strand (18); Chain (3); Disulfide bond (6); Glycosylation (1); Helix (13); Modified residue (1); Natural variant (4); Propeptide (2); Sequence conflict (1); Signal peptide (1); Turn (4)
Keywords 3D-structure;Acetylation;Cell membrane;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Membrane;Protease;Reference proteome;Secreted;Signal;Thiol protease;Zymogen
Interact With Q6UY14; P02760; Q96IK1-2; P40692; Q9NYA1
Induction
Subcellular Location SUBCELLULAR LOCATION: Lysosome {ECO:0000269|PubMed:16733801}. Melanosome {ECO:0000269|PubMed:17081065}. Secreted, extracellular space {ECO:0000250|UniProtKB:A1E295}. Apical cell membrane {ECO:0000250|UniProtKB:P10605}; Peripheral membrane protein {ECO:0000250|UniProtKB:P10605}; Extracellular side {ECO:0000250|UniProtKB:P10605}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Localizes to the lumen of thyroid follicles and to the apical membrane of thyroid epithelial cells (By similarity). {ECO:0000250|UniProtKB:P10605, ECO:0000269|PubMed:17081065}.
Modified Residue MOD_RES 220; /note=N6-acetyllysine; /evidence=ECO:0000250|UniProtKB:P10605
Post Translational Modification
Signal Peptide SIGNAL 1..17; /evidence=ECO:0000255
Structure 3D X-ray crystallography (14)
Cross Reference PDB 1CSB; 1GMY; 1HUC; 1PBH; 2IPP; 2PBH; 3AI8; 3CBJ; 3CBK; 3K9M; 3PBH; 5MBL; 5MBM; 6AY2;
Mapped Pubmed ID 10072072; 10631941; 10748235; 10809954; 10966814; 11119712; 11513559; 11684289; 11741472; 11813165; 11815600; 11853874; 11932257; 12072442; 12078484; 12086583; 12102727; 12185082; 12201820; 12437120; 12581740; 12589965; 12677446; 12726991; 12742663; 12748383; 12887051; 12893746; 12926111; 1326552; 14729603; 14730346; 14984956; 15016552; 15100281; 15122332; 15255544; 15262981; 15274632; 15512772; 15679122; 15799821; 15807897; 15816632; 15831716; 15832773; 15950618; 16034129; 16051222; 16077201; 16169070; 16181339; 16237761; 16315320; 16364318; 16381007; 16492714; 16534247; 16709808; 16894574; 16913838; 16914553; 17064696; 17504810; 17507477; 17519890; 17724614; 17726009; 17982689; 17990360; 17991740; 18163891; 18166152; 18296264; 18314113; 18472965; 18515357; 18543249; 18566385; 18566436; 18598236; 18616803; 18706099; 18714186; 18760860; 18820679; 18931679; 18949742; 19004543; 19040356; 19331819; 19414800; 19434518; 19454696; 19615732; 19661440; 19692168; 19700239; 19774387; 19913121; 19915865; 19930869; 20008285; 20042316; 20180636; 20302512; 20424577; 20536394; 20563251; 20567828; 20584670; 20627011; 20628086; 20699092; 20706999; 20711500; 20833970; 20860624; 20880494; 20930303; 21092200; 21112096; 21199580; 21250859; 21323990; 21402738; 21508263; 21562164; 21585286; 21598397; 21600167; 21742978; 21835790; 21840777; 21850018; 21880013; 21900206; 21925292; 21945151; 21948970; 21967108; 22066471; 22127599; 22190034; 22209964; 22222211; 22262857; 22266111; 22278224; 22287159; 22322590; 22384200; 22464443; 22467319; 22571763; 22695494; 22732093; 22745374; 22782900; 22851129; 22949512; 23024364; 23063511; 23189279; 23195957; 23219593; 23222509; 23222817; 23292187; 23362198; 23466190; 23591598; 23602568; 23603447; 23633927; 23650620; 23667900; 23677785; 23708264; 23867824; 24083575; 24108784; 24138030; 24139065; 24166050; 24215712; 24595198; 24618814; 24699410; 24749816; 24790080; 25036637; 25095637; 25106406; 25209871; 25260629; 25452305; 25502766; 25572981; 25577554; 25633482; 25640309; 25802234; 25883214; 25959927; 25991043; 26081835; 26638075; 26904684; 26943237; 26995190; 27031837; 27101784; 27260469; 27345423; 27398911; 27526672; 27630170; 27648120; 27729455; 27902765; 28074340; 28182342; 28383558; 28389621; 28440429; 28478025; 28495172; 28951461; 29046353; 29149174; 29227683; 29935187; 30046941; 30099343; 30261474; 30530526; 30575263; 30679571; 30818851; 30833610; 30968153; 30982660; 31221819; 31371266; 31755958; 31932607; 32242285; 32305689; 32673819; 32754840; 32979864; 33268027; 33293479; 33303550; 33315943; 33333840; 33628106; 33964876; 34093436; 34407143; 7718586; 8687433; 9008168; 9539769; 9545226;
Motif
Gene Encoded By
Mass 37,822
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda 3.4.22.1;