Detail Information for IndEnz0002018921
IED ID IndEnz0002018921
Enzyme Type ID protease018921
Protein Name Cytosolic carboxypeptidase 2
EC 3.4.17.-
ATP/GTP-binding protein-like 2
Protein deglutamylase CCP2
Testis-expressed protein 25
Gene Name zte25 agbl2 ccp2 zgc:165648
Organism Danio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio)
Enzyme Sequence MQKKSDDPYERLLLLHLQHYGFLSDNSSGIPIKREWEGIQDSTASDMINSSSPSESSDSNLEEEQEESKPCSNFFSLNKALRTRQLVFDFDGERPIPRLRDPLDLFTIPSTSCPFQGVRWPIECDVICDKIQHIEWDPSEPETFYQPTGNEQTPMPVGEVRGNTVYCIDPATKASSFTYSRVGGSRGPIKSATSCANNQKEPTLAFESRFECGNLQKAVQVGQYDYGLTLRTDLYTTKHTQWFYFRVRNMREGVTYRFTIINLMKSSSLYGAGMCPLLYSEKTAWLKGEGWKRTGSSIRYYRNNIEQDGKALYSLTWTLEFPYDGDTCYLAHCYPYTYSKLQHYLREVISDPVRAAYCKLRVLCRSLAGNAVYVLTITAPSSSLAERKAKRAVVVTARVHPGETNGSWMMQGFLEFLLSDLPDAHLLRETFIFKVIPMLNPDGVVVGNYRCSLAGRDLNRNYRSMLRDSFPCIWYTRNMVKRLLAEREVVVYCDFHGHSRKNNVFMYGCNERKDASQCLQERVFPLMMSKNAKDKFSFRSCKFKMHKSKEGTGRIVMWRLGIRNSYTMESTFGGSTLGDRKGTHFSTLDLKSMGYCFCDTLLDFCDPDPAKMTRCLEELGVLLKQEIRRKLGREVDSLENLSDIDIESSTSGSNSTESDGLPVHLLNVTNQGKKKLLRSRKERNRLRQGRVQSAGKTDASKPYSCQTLNATTQHGDTEDQS
Enzyme Length 721
Uniprot Accession Number A6H8T7
Absorption
Active Site ACT_SITE 450; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P00730
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=(L-glutamyl)(n+1)-gamma-L-glutamyl-L-glutamyl-[protein] + H2O = (L-glutamyl)(n)-gamma-L-glutamyl-L-glutamyl-[protein] + L-glutamate; Xref=Rhea:RHEA:60004, Rhea:RHEA-COMP:15519, Rhea:RHEA-COMP:15675, ChEBI:CHEBI:15377, ChEBI:CHEBI:29985, ChEBI:CHEBI:143623; Evidence={ECO:0000250|UniProtKB:Q8CDK2};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60005; Evidence={ECO:0000250|UniProtKB:Q8CDK2};
DNA Binding
EC Number 3.4.17.-
Enzyme Function FUNCTION: Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Does not show detyrosinase or deglycylase activities from the carboxy-terminus of tubulin. {ECO:0000250|UniProtKB:Q8CDK2}.; FUNCTION: Metallocarboxypeptidase that mediates deglutamylation of tubulin and non-tubulin target proteins. Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of tubulin protein. Specifically cleaves tubulin long-side-chains, while it is not able to remove the branching point glutamate. Also catalyzes the removal of polyglutamate residues from the carboxy-terminus of non-tubulin proteins. {ECO:0000250|UniProtKB:Q8CDK2}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Compositional bias (3); Metal binding (3); Region (2)
Keywords Carboxypeptidase;Cell projection;Cytoplasm;Cytoskeleton;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q8CDK2}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:Q5U5Z8}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q5U5Z8}. Note=Colocalizes with gamma-tubulin in the centrioles and with glutamylated tubulin in the basal bodies of ciliated cells. {ECO:0000250|UniProtKB:Q5U5Z8}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 24022483; 24743595; 30899431;
Motif
Gene Encoded By
Mass 82,236
Kinetics
Metal Binding METAL 400; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q09M02; METAL 403; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q09M02; METAL 496; /note=Zinc; /evidence=ECO:0000250|UniProtKB:P00730
Rhea ID RHEA:60004; RHEA:60005
Cross Reference Brenda