| IED ID | IndEnz0002018921 |
| Enzyme Type ID | protease018921 |
| Protein Name |
Cytosolic carboxypeptidase 2 EC 3.4.17.- ATP/GTP-binding protein-like 2 Protein deglutamylase CCP2 Testis-expressed protein 25 |
| Gene Name | zte25 agbl2 ccp2 zgc:165648 |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MQKKSDDPYERLLLLHLQHYGFLSDNSSGIPIKREWEGIQDSTASDMINSSSPSESSDSNLEEEQEESKPCSNFFSLNKALRTRQLVFDFDGERPIPRLRDPLDLFTIPSTSCPFQGVRWPIECDVICDKIQHIEWDPSEPETFYQPTGNEQTPMPVGEVRGNTVYCIDPATKASSFTYSRVGGSRGPIKSATSCANNQKEPTLAFESRFECGNLQKAVQVGQYDYGLTLRTDLYTTKHTQWFYFRVRNMREGVTYRFTIINLMKSSSLYGAGMCPLLYSEKTAWLKGEGWKRTGSSIRYYRNNIEQDGKALYSLTWTLEFPYDGDTCYLAHCYPYTYSKLQHYLREVISDPVRAAYCKLRVLCRSLAGNAVYVLTITAPSSSLAERKAKRAVVVTARVHPGETNGSWMMQGFLEFLLSDLPDAHLLRETFIFKVIPMLNPDGVVVGNYRCSLAGRDLNRNYRSMLRDSFPCIWYTRNMVKRLLAEREVVVYCDFHGHSRKNNVFMYGCNERKDASQCLQERVFPLMMSKNAKDKFSFRSCKFKMHKSKEGTGRIVMWRLGIRNSYTMESTFGGSTLGDRKGTHFSTLDLKSMGYCFCDTLLDFCDPDPAKMTRCLEELGVLLKQEIRRKLGREVDSLENLSDIDIESSTSGSNSTESDGLPVHLLNVTNQGKKKLLRSRKERNRLRQGRVQSAGKTDASKPYSCQTLNATTQHGDTEDQS |
| Enzyme Length | 721 |
| Uniprot Accession Number | A6H8T7 |
| Absorption | |
| Active Site | ACT_SITE 450; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P00730 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(L-glutamyl)(n+1)-gamma-L-glutamyl-L-glutamyl-[protein] + H2O = (L-glutamyl)(n)-gamma-L-glutamyl-L-glutamyl-[protein] + L-glutamate; Xref=Rhea:RHEA:60004, Rhea:RHEA-COMP:15519, Rhea:RHEA-COMP:15675, ChEBI:CHEBI:15377, ChEBI:CHEBI:29985, ChEBI:CHEBI:143623; Evidence={ECO:0000250|UniProtKB:Q8CDK2};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60005; Evidence={ECO:0000250|UniProtKB:Q8CDK2}; |
| DNA Binding | |
| EC Number | 3.4.17.- |
| Enzyme Function | FUNCTION: Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Does not show detyrosinase or deglycylase activities from the carboxy-terminus of tubulin. {ECO:0000250|UniProtKB:Q8CDK2}.; FUNCTION: Metallocarboxypeptidase that mediates deglutamylation of tubulin and non-tubulin target proteins. Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of tubulin protein. Specifically cleaves tubulin long-side-chains, while it is not able to remove the branching point glutamate. Also catalyzes the removal of polyglutamate residues from the carboxy-terminus of non-tubulin proteins. {ECO:0000250|UniProtKB:Q8CDK2}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (3); Metal binding (3); Region (2) |
| Keywords | Carboxypeptidase;Cell projection;Cytoplasm;Cytoskeleton;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q8CDK2}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:Q5U5Z8}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q5U5Z8}. Note=Colocalizes with gamma-tubulin in the centrioles and with glutamylated tubulin in the basal bodies of ciliated cells. {ECO:0000250|UniProtKB:Q5U5Z8}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 24022483; 24743595; 30899431; |
| Motif | |
| Gene Encoded By | |
| Mass | 82,236 |
| Kinetics | |
| Metal Binding | METAL 400; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q09M02; METAL 403; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q09M02; METAL 496; /note=Zinc; /evidence=ECO:0000250|UniProtKB:P00730 |
| Rhea ID | RHEA:60004; RHEA:60005 |
| Cross Reference Brenda |