Detail Information for IndEnz0002018937
IED ID IndEnz0002018937
Enzyme Type ID protease018937
Protein Name Carboxypeptidase D
EC 3.4.17.22
Metallocarboxypeptidase D
Protein silver
Gene Name svr CPD CpepE CG4122
Organism Drosophila melanogaster (Fruit fly)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly)
Enzyme Sequence MPTLGLLFASIGIAVLAMGVPHCRGYTIKEDESFLQQPHYASQEQLEDLFAGLEKAYPNQAKVHFLGRSLEGRNLLALQISRNTRSRNLLTPPVKYIANMHGDETVGRQLLVYMAQYLLGNHERISDLGQLVNSTDIYLVPTMNPDGYALSQEGNCESLPNYVGRGNAANIDLNRDFPDRLEQSHVHQLRAQSRQPETAALVNWIVSKPFVLSANFHGGAVVASYPYDNSLAHNECCEESLTPDDRVFKQLAHTYSDNHPIMRKGNNCNDSFSGGITNGAHWYELSGGMQDFNYAFSNCFELTIELSCCKYPAASTLPQEWQRNKASLLQLLRQAHIGIKGLVTDASGFPIADANVYVAGLEEKPMRTSKRGEYWRLLTPGLYSVHASAFGYQTSAPQQVRVTNDNQEALRLDFKLAPVETNFDGNFRKVKVERSEPPQKLKKQFNGFLTPTKYEHHNFTAMESYLRAISSSYPSLTRLYSIGKSVQGRDLWVLEIFATPGSHVPGVPEFKYVANMHGNEVVGKELLLILTKYMLERYGNDDRITKLVNGTRMHFLYSMNPDGYEISIEGDRTGGVGRANAHGIDLNRNFPDQYGTDRFNKVTEPEVAAVMNWTLSLPFVLSANLHGGSLVANYPFDDNENDFNDPFMRLRNSSINGRKPNPTEDNALFKHLAGIYSNAHPTMYLGQPCELFQNEFFPDGITNGAQWYSVTGGMQDWNYVRAGCLELTIEMGCDKFPKAAELSRYWEDHREPLLQFIEQVHCGIHGFVHSTIGTPIAGAVVRLDGANHSTYSQVFGDYWKLALPGRHNLTVLGDNYAPLRMEVEVPDVHPFEMRMDITLMPDDPQHWASANDFRIIENVVNTRYHTNPQVRARLAELENQNGQIASFGYADSEFGTIFNYLKMTSDIGEPEEHKYKLLVVSSLYDTTAPLGREILLNLIRHLVEGFKLQDTSVVELLKRSVIYFLPQTSKFQNVFDMYNSNTSICDPVLGDELAERILGPETDQAKDVFLQFLRSERFDLMLTFGAGNSDLNYPKGDSVLVKFAHRMQRTEFNYSPLQCPPSATRQLHRETTERLTNMMYRIYNLPVYTLGISCCRMPHQKKIASVWRKNIDKIKNFLALVKTGVSGLVQNDKGQPLREAYVRLLEHDRIINVTKNVARFQLMLPHGLYGLEVTAPNYESQMIKVDVEDGRVTELGIIRMHPFTLIRGVVLELPNNDNRATTSIAGVVLDESNHPVRNAKVSVVGQTQLRNFTGSMGQYRISAVPLGTITLKVEAPRHLEATRQMHLIQGGLATENVVFHLKVNEHVFGLPRFLFILCASVLIIVGVIVCVLCAQFWFYRRHRGDKPYYNFSLLPQRGKEQFGLEDDDGGDDGETELFRSPIKRELSQRAHLVNNQTNYSFIIQAA
Enzyme Length 1406
Uniprot Accession Number P42787
Absorption
Active Site ACT_SITE 305; /note=Proton donor/acceptor 1; /evidence=ECO:0000250|UniProtKB:Q90240; ACT_SITE 730; /note=Proton donor/acceptor 2; /evidence=ECO:0000250|UniProtKB:Q90240
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Releases C-terminal Arg and Lys from polypeptides.; EC=3.4.17.22;
DNA Binding
EC Number 3.4.17.22
Enzyme Function FUNCTION: Required for the proper melanization and sclerotization of the cuticle. {ECO:0000269|PubMed:12393882, ECO:0000269|PubMed:7568156, ECO:0000269|PubMed:8000074}.
Temperature Dependency
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.0 for carboxypeptidase domain 1, and 5.0-6.0 for domain 2.;
Pathway
nucleotide Binding
Features Active site (2); Alternative sequence (5); Chain (1); Frameshift (1); Glycosylation (11); Metal binding (6); Modified residue (1); Motif (1); Region (3); Sequence caution (1); Sequence conflict (3); Signal peptide (1); Topological domain (2); Transmembrane (1)
Keywords Alternative splicing;Carboxypeptidase;Cuticle;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Phosphoprotein;Protease;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix;Zinc
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}.
Modified Residue MOD_RES 1380; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897
Post Translational Modification
Signal Peptide SIGNAL 1..25; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 11337470; 11744370; 11779831; 11901120; 12824434; 14605208; 14657024; 15466469; 16336044; 16556608; 16578132; 16676361; 17246894; 17247082; 17936709; 18245849; 19012054; 1925579; 19317464; 1996129; 20214937; 20290998; 20386952; 20600119; 2128440; 21718515; 23071443; 23173095; 24025855; 2499512; 25165137; 25258387; 25312911; 25634895; 26021350; 26378456; 27430952; 27582081; 27794539; 28512187; 30366088; 3091446; 3124532; 31309630; 31915294; 33159074; 33264304; 404122; 6291042; 6408464; 6806144; 8369138; 8580497; 8785418;
Motif MOTIF 1343..1345; /note=Cell attachment site; /evidence=ECO:0000255
Gene Encoded By
Mass 158,789
Kinetics
Metal Binding METAL 101; /note=Zinc 1; catalytic; /evidence=ECO:0000250; METAL 104; /note=Zinc 1; catalytic; /evidence=ECO:0000250; METAL 217; /note=Zinc 1; catalytic; /evidence=ECO:0000250; METAL 517; /note=Zinc 2; catalytic; /evidence=ECO:0000250|UniProtKB:P00730; METAL 520; /note=Zinc 2; catalytic; /evidence=ECO:0000250|UniProtKB:P00730; METAL 626; /note=Zinc 2; catalytic; /evidence=ECO:0000250|UniProtKB:P00730
Rhea ID
Cross Reference Brenda 3.4.17.22;