| IED ID | IndEnz0002018937 |
| Enzyme Type ID | protease018937 |
| Protein Name |
Carboxypeptidase D EC 3.4.17.22 Metallocarboxypeptidase D Protein silver |
| Gene Name | svr CPD CpepE CG4122 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MPTLGLLFASIGIAVLAMGVPHCRGYTIKEDESFLQQPHYASQEQLEDLFAGLEKAYPNQAKVHFLGRSLEGRNLLALQISRNTRSRNLLTPPVKYIANMHGDETVGRQLLVYMAQYLLGNHERISDLGQLVNSTDIYLVPTMNPDGYALSQEGNCESLPNYVGRGNAANIDLNRDFPDRLEQSHVHQLRAQSRQPETAALVNWIVSKPFVLSANFHGGAVVASYPYDNSLAHNECCEESLTPDDRVFKQLAHTYSDNHPIMRKGNNCNDSFSGGITNGAHWYELSGGMQDFNYAFSNCFELTIELSCCKYPAASTLPQEWQRNKASLLQLLRQAHIGIKGLVTDASGFPIADANVYVAGLEEKPMRTSKRGEYWRLLTPGLYSVHASAFGYQTSAPQQVRVTNDNQEALRLDFKLAPVETNFDGNFRKVKVERSEPPQKLKKQFNGFLTPTKYEHHNFTAMESYLRAISSSYPSLTRLYSIGKSVQGRDLWVLEIFATPGSHVPGVPEFKYVANMHGNEVVGKELLLILTKYMLERYGNDDRITKLVNGTRMHFLYSMNPDGYEISIEGDRTGGVGRANAHGIDLNRNFPDQYGTDRFNKVTEPEVAAVMNWTLSLPFVLSANLHGGSLVANYPFDDNENDFNDPFMRLRNSSINGRKPNPTEDNALFKHLAGIYSNAHPTMYLGQPCELFQNEFFPDGITNGAQWYSVTGGMQDWNYVRAGCLELTIEMGCDKFPKAAELSRYWEDHREPLLQFIEQVHCGIHGFVHSTIGTPIAGAVVRLDGANHSTYSQVFGDYWKLALPGRHNLTVLGDNYAPLRMEVEVPDVHPFEMRMDITLMPDDPQHWASANDFRIIENVVNTRYHTNPQVRARLAELENQNGQIASFGYADSEFGTIFNYLKMTSDIGEPEEHKYKLLVVSSLYDTTAPLGREILLNLIRHLVEGFKLQDTSVVELLKRSVIYFLPQTSKFQNVFDMYNSNTSICDPVLGDELAERILGPETDQAKDVFLQFLRSERFDLMLTFGAGNSDLNYPKGDSVLVKFAHRMQRTEFNYSPLQCPPSATRQLHRETTERLTNMMYRIYNLPVYTLGISCCRMPHQKKIASVWRKNIDKIKNFLALVKTGVSGLVQNDKGQPLREAYVRLLEHDRIINVTKNVARFQLMLPHGLYGLEVTAPNYESQMIKVDVEDGRVTELGIIRMHPFTLIRGVVLELPNNDNRATTSIAGVVLDESNHPVRNAKVSVVGQTQLRNFTGSMGQYRISAVPLGTITLKVEAPRHLEATRQMHLIQGGLATENVVFHLKVNEHVFGLPRFLFILCASVLIIVGVIVCVLCAQFWFYRRHRGDKPYYNFSLLPQRGKEQFGLEDDDGGDDGETELFRSPIKRELSQRAHLVNNQTNYSFIIQAA |
| Enzyme Length | 1406 |
| Uniprot Accession Number | P42787 |
| Absorption | |
| Active Site | ACT_SITE 305; /note=Proton donor/acceptor 1; /evidence=ECO:0000250|UniProtKB:Q90240; ACT_SITE 730; /note=Proton donor/acceptor 2; /evidence=ECO:0000250|UniProtKB:Q90240 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Releases C-terminal Arg and Lys from polypeptides.; EC=3.4.17.22; |
| DNA Binding | |
| EC Number | 3.4.17.22 |
| Enzyme Function | FUNCTION: Required for the proper melanization and sclerotization of the cuticle. {ECO:0000269|PubMed:12393882, ECO:0000269|PubMed:7568156, ECO:0000269|PubMed:8000074}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.0 for carboxypeptidase domain 1, and 5.0-6.0 for domain 2.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (5); Chain (1); Frameshift (1); Glycosylation (11); Metal binding (6); Modified residue (1); Motif (1); Region (3); Sequence caution (1); Sequence conflict (3); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | Alternative splicing;Carboxypeptidase;Cuticle;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Phosphoprotein;Protease;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}. |
| Modified Residue | MOD_RES 1380; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897 |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11337470; 11744370; 11779831; 11901120; 12824434; 14605208; 14657024; 15466469; 16336044; 16556608; 16578132; 16676361; 17246894; 17247082; 17936709; 18245849; 19012054; 1925579; 19317464; 1996129; 20214937; 20290998; 20386952; 20600119; 2128440; 21718515; 23071443; 23173095; 24025855; 2499512; 25165137; 25258387; 25312911; 25634895; 26021350; 26378456; 27430952; 27582081; 27794539; 28512187; 30366088; 3091446; 3124532; 31309630; 31915294; 33159074; 33264304; 404122; 6291042; 6408464; 6806144; 8369138; 8580497; 8785418; |
| Motif | MOTIF 1343..1345; /note=Cell attachment site; /evidence=ECO:0000255 |
| Gene Encoded By | |
| Mass | 158,789 |
| Kinetics | |
| Metal Binding | METAL 101; /note=Zinc 1; catalytic; /evidence=ECO:0000250; METAL 104; /note=Zinc 1; catalytic; /evidence=ECO:0000250; METAL 217; /note=Zinc 1; catalytic; /evidence=ECO:0000250; METAL 517; /note=Zinc 2; catalytic; /evidence=ECO:0000250|UniProtKB:P00730; METAL 520; /note=Zinc 2; catalytic; /evidence=ECO:0000250|UniProtKB:P00730; METAL 626; /note=Zinc 2; catalytic; /evidence=ECO:0000250|UniProtKB:P00730 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.17.22; |