Detail Information for IndEnz0002018940
IED ID IndEnz0002018940
Enzyme Type ID protease018940
Protein Name Carboxypeptidase E
CPE
EC 3.4.17.10
Carboxypeptidase H
CPH
Enkephalin convertase
Prohormone-processing carboxypeptidase
Gene Name Cpe
Organism Rattus norvegicus (Rat)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat)
Enzyme Sequence MAGRGGRVLLALCAALVAGGWLLAAEAQEPGAPAAGMRRRRRLQQEDGISFEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVHEPGEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQRGNETIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNLKKIVDQNSKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGTAHEYSSCPDDAIFQSLARAYSSFNPVMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPPEETLKSYWEDNKNSLINYLEQIHRGVKGFVRDLQGNPIANATISVDGIDHDVTSAKDGDYWRLLVPGNYKLTASAPGYLAITKKVAVPFSPAVGVDFELESFSERKEEEKEELMEWWKMMSETLNF
Enzyme Length 476
Uniprot Accession Number P15087
Absorption
Active Site ACT_SITE 342; /note=Proton donor/acceptor; /evidence=ECO:0000250|UniProtKB:P14384
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of C-terminal arginine or lysine residues from polypeptides.; EC=3.4.17.10;
DNA Binding
EC Number 3.4.17.10
Enzyme Function FUNCTION: Sorting receptor that directs prohormones to the regulated secretory pathway. Acts also as a prohormone processing enzyme in neuro/endocrine cells, removing dibasic residues from the C-terminal end of peptide hormone precursors after initial endoprotease cleavage. {ECO:0000250|UniProtKB:Q00493}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Glycosylation (2); Metal binding (3); Propeptide (1); Sequence conflict (3); Signal peptide (1)
Keywords Carboxypeptidase;Cleavage on pair of basic residues;Cytoplasmic vesicle;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle {ECO:0000269|PubMed:14597614}. Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000269|PubMed:14597614}; Peripheral membrane protein {ECO:0000269|PubMed:14597614}. Secreted {ECO:0000269|PubMed:14597614}. Note=Associated with the secretory granule membrane through direct binding to lipid rafts in intragranular conditions. {ECO:0000269|PubMed:14597614}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..27; /note=Or 34
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10206965; 10386951; 15233624; 16219686; 17543468; 19166515; 19419578; 19553464; 2822027; 6326144; 9173892;
Motif
Gene Encoded By
Mass 53,309
Kinetics
Metal Binding METAL 114; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P00730; METAL 117; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P00730; METAL 248; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P00730
Rhea ID
Cross Reference Brenda