Detail Information for IndEnz0002018966
IED ID IndEnz0002018966
Enzyme Type ID protease018966
Protein Name Probable vacuolar protease A
EC 3.4.23.25
Aspartic endopeptidase PEP2
Aspartic protease PEP2
Gene Name PEP2 ARB_02919
Organism Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) (Trichophyton mentagrophytes)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Onygenales Arthrodermataceae (dermatophytes) Trichophyton Arthroderma benhamiae (Trichophyton mentagrophytes) Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) (Trichophyton mentagrophytes)
Enzyme Sequence MKGSLLLAGATLLGCTSAKLHSLKLKKVSLKEQLEHADIDVQIKSLGQKYMGIRPEQHEQQMFKEQTPIEVESGHNVLIDNFLNAQYFSEISIGTPPQTFKVVLDTGSSNLWVPGKDCSSIACFLHSTYDSSASSTYSKNGTKFAIRYGSGSLEGFVSRDSVKIGDMTIKKQLFAEATSEPGLAFAFGRFDGIMGMGFSSISVNGITPPFYNMIDQGLIDEPVFSFYLGDTNKDGDQSVVTFGGSDTNHFTGDMTTIPLRRKAYWEVDFDAISLGKDTAALENTGIILDTGTSLIALPTTLAEMINTQIGATKSWNGQYTLDCAKRDSLPDVTFTLSGHNFTIGPHDYTLEVSGTCISSFMGMDFPEPVGPLAILGDSFLRRYYSVYDLGKGTVGLAKAK
Enzyme Length 400
Uniprot Accession Number D4B385
Absorption
Active Site ACT_SITE 105; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094; ACT_SITE 289; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins with broad specificity for peptide bonds. Cleaves -Leu-Leu-|-Val-Tyr- bond in a synthetic substrate. Does not act on esters of Tyr or Arg.; EC=3.4.23.25;
DNA Binding
EC Number 3.4.23.25
Enzyme Function FUNCTION: Vacuolar aspartic endopeptidase which is probably also secreted and contributes to virulence. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Disulfide bond (2); Domain (1); Glycosylation (2); Propeptide (1); Signal peptide (1)
Keywords Aspartyl protease;Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Signal;Vacuole;Virulence;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Vacuole lumen {ECO:0000250}. Secreted {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..18; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 43,416
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda