Detail Information for IndEnz0002018969
IED ID IndEnz0002018969
Enzyme Type ID protease018969
Protein Name Mucorpepsin
EC 3.4.23.23
Mucor rennin
Gene Name
Organism Rhizomucor miehei
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Fungi incertae sedis Mucoromycota Mucoromycotina Mucoromycetes Mucorales (pin molds) Lichtheimiaceae Rhizomucor Rhizomucor miehei
Enzyme Sequence MLFSQITSAILLTAASLSLTTARPVSKQSESKDKLLALPLTSVSRKFSQTKFGQQQLAEKLAGLKPFSEAAADGSVDTPGYYDFDLEEYAIPVSIGTPGQDFLLLFDTGSSDTWVPHKGCTKSEGCVGSRFFDPSASSTFKATNYNLNITYGTGGANGLYFEDSIAIGDITVTKQILAYVDNVRGPTAEQSPNADIFLDGLFGAAYPDNTAMEAEYGSTYNTVHVNLYKQGLISSPLFSVYMNTNSGTGEVVFGGVNNTLLGGDIAYTDVMSRYGGYYFWDAPVTGITVDGSAAVRFSRPQAFTIDTGTNFFIMPSSAASKIVKAALPDATETQQGWVVPCASYQNSKSTISIVMQKSGSSSDTIEISVPVSKMLLPVDQSNETCMFIILPDGGNQYIVGNLFLRFFVNVYDFGNNRIGFAPLASAYENE
Enzyme Length 430
Uniprot Accession Number P00799
Absorption
Active Site ACT_SITE 107; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094; ACT_SITE 306; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins, favoring hydrophobic residues at P1 and P1'. Clots milk. Does not accept Lys at P1, and hence does not activate trypsinogen.; EC=3.4.23.23;
DNA Binding
EC Number 3.4.23.23
Enzyme Function FUNCTION: This enzyme, capable of clotting milk is frequently used for cheese production.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Beta strand (26); Chain (1); Disulfide bond (2); Domain (1); Glycosylation (2); Helix (11); Natural variant (1); Propeptide (1); Sequence conflict (8); Signal peptide (1); Turn (5)
Keywords 3D-structure;Aspartyl protease;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Protease;Signal;Zymogen
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..22
Structure 3D X-ray crystallography (2)
Cross Reference PDB 2ASI; 2RMP;
Mapped Pubmed ID 10089458;
Motif
Gene Encoded By
Mass 46,168
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda