| IED ID | IndEnz0002019001 |
| Enzyme Type ID | protease019001 |
| Protein Name |
ATP-dependent zinc metalloprotease FTSH 8, chloroplastic AtFTSH8 EC 3.4.24.- |
| Gene Name | FTSH8 At1g06430 F12K11.22 F12K11_24 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAASSACLLGNGLSVYTTKQRFQKLGLDRTSKVTVVKASLDEKKHEGRRGFFKLLLGNAAAGVGLLASGNANADEQGQGVSSSRMSYSRFLEYLDKGRVEKVDLYENGTIAIVEAVSPELGNRIQRVRVQLPGLSQELLQKLRAKNIDFAAHNAQEDQGSPILNLIGNLAFPVILIGGLFLLSRRSSGGMGGPGGPGFPLQIGQSKAKFQMEPNTGVTFDDVAGVDEAKQDFMEVVEFLKKPERFTAVGARIPKGVLLVGPPGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQLLTEMDGFEGNTGVIVVAATNRADILDSALLRPGRFDRQVSVDVPDVKGRTDILKVHSGNKKFESGVSLEVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGTVMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLIPRGQARGLTWFIPSDDPTLISKQQLFARIVGGLGGRAAEEVIFGESEVTTGAVSDLQQITGLAKQMVTTFGMSEIGPWSLMDSSEQSDVIMRMMARNSMSEKLANDIDTAVKTLSDKAYEIALSQIRNNREAMDKIVEILLEKETMSGDEFRAILSEFTEIPPENRVASSTSTSTPTPASV |
| Enzyme Length | 685 |
| Uniprot Accession Number | Q8W585 |
| Absorption | |
| Active Site | ACT_SITE 482; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Part of a complex that function as an ATP-dependent zinc metallopeptidase. Involved in the thylakoid formation and in the removal of damaged D1 in the photosystem II, preventing cell death under high-intensity light conditions. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 260..267; /note=ATP; /evidence=ECO:0000255 |
| Features | Active site (1); Chain (1); Metal binding (3); Nucleotide binding (1); Topological domain (2); Transit peptide (2); Transmembrane (1) |
| Keywords | ATP-binding;Chloroplast;Developmental protein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Nucleotide-binding;Plastid;Protease;Reference proteome;Thylakoid;Transit peptide;Transmembrane;Transmembrane helix;Zinc |
| Interact With | |
| Induction | INDUCTION: By heat and high light. {ECO:0000269|PubMed:15266057}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane {ECO:0000269|PubMed:14630971, ECO:0000269|PubMed:18431481}; Single-pass membrane protein {ECO:0000269|PubMed:14630971}; Stromal side {ECO:0000269|PubMed:14630971}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11826309; 12185496; 12938931; 14729914; 14730085; 15028209; 15047901; 15235117; 15247402; 15322131; 16157880; 16330762; 16500996; 16603408; 16972165; 17181860; 17217462; 17432890; 18230142; 18633119; 19228339; 20118269; 21062893; 21214651; 22121866; 23548781; 26909098; 28687655; 29973948; 30131421; |
| Motif | |
| Gene Encoded By | |
| Mass | 73,198 |
| Kinetics | |
| Metal Binding | METAL 481; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 485; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 559; /note=Zinc; catalytic; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.B20; |