Detail Information for IndEnz0002019001
IED ID IndEnz0002019001
Enzyme Type ID protease019001
Protein Name ATP-dependent zinc metalloprotease FTSH 8, chloroplastic
AtFTSH8
EC 3.4.24.-
Gene Name FTSH8 At1g06430 F12K11.22 F12K11_24
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MAASSACLLGNGLSVYTTKQRFQKLGLDRTSKVTVVKASLDEKKHEGRRGFFKLLLGNAAAGVGLLASGNANADEQGQGVSSSRMSYSRFLEYLDKGRVEKVDLYENGTIAIVEAVSPELGNRIQRVRVQLPGLSQELLQKLRAKNIDFAAHNAQEDQGSPILNLIGNLAFPVILIGGLFLLSRRSSGGMGGPGGPGFPLQIGQSKAKFQMEPNTGVTFDDVAGVDEAKQDFMEVVEFLKKPERFTAVGARIPKGVLLVGPPGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQLLTEMDGFEGNTGVIVVAATNRADILDSALLRPGRFDRQVSVDVPDVKGRTDILKVHSGNKKFESGVSLEVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGTVMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLIPRGQARGLTWFIPSDDPTLISKQQLFARIVGGLGGRAAEEVIFGESEVTTGAVSDLQQITGLAKQMVTTFGMSEIGPWSLMDSSEQSDVIMRMMARNSMSEKLANDIDTAVKTLSDKAYEIALSQIRNNREAMDKIVEILLEKETMSGDEFRAILSEFTEIPPENRVASSTSTSTPTPASV
Enzyme Length 685
Uniprot Accession Number Q8W585
Absorption
Active Site ACT_SITE 482; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.24.-
Enzyme Function FUNCTION: Part of a complex that function as an ATP-dependent zinc metallopeptidase. Involved in the thylakoid formation and in the removal of damaged D1 in the photosystem II, preventing cell death under high-intensity light conditions.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding NP_BIND 260..267; /note=ATP; /evidence=ECO:0000255
Features Active site (1); Chain (1); Metal binding (3); Nucleotide binding (1); Topological domain (2); Transit peptide (2); Transmembrane (1)
Keywords ATP-binding;Chloroplast;Developmental protein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Nucleotide-binding;Plastid;Protease;Reference proteome;Thylakoid;Transit peptide;Transmembrane;Transmembrane helix;Zinc
Interact With
Induction INDUCTION: By heat and high light. {ECO:0000269|PubMed:15266057}.
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane {ECO:0000269|PubMed:14630971, ECO:0000269|PubMed:18431481}; Single-pass membrane protein {ECO:0000269|PubMed:14630971}; Stromal side {ECO:0000269|PubMed:14630971}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 11826309; 12185496; 12938931; 14729914; 14730085; 15028209; 15047901; 15235117; 15247402; 15322131; 16157880; 16330762; 16500996; 16603408; 16972165; 17181860; 17217462; 17432890; 18230142; 18633119; 19228339; 20118269; 21062893; 21214651; 22121866; 23548781; 26909098; 28687655; 29973948; 30131421;
Motif
Gene Encoded By
Mass 73,198
Kinetics
Metal Binding METAL 481; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 485; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 559; /note=Zinc; catalytic; /evidence=ECO:0000250
Rhea ID
Cross Reference Brenda 3.4.24.B20;