Detail Information for IndEnz0002019029
IED ID IndEnz0002019029
Enzyme Type ID protease019029
Protein Name Probable aspartic proteinase GIP2
EC 3.4.23.-
Glucanase inhibitor protein 2
NbGIP2
Gene Name GIP2 Niben101Scf03191g04002
Organism Nicotiana benthamiana
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Nicotianoideae Nicotianeae Nicotiana Nicotiana benthamiana
Enzyme Sequence MASSCCLHAILLCSLLFITSTTAQSETSFRPKGLILPITKDALTLQYLTQIQQRTPLVPVSLTLDLGGQFLWVDCDQGYVSSTYRPARCRSAQCSLAGAGSGCGQCFSPPKPGCNNNTCGLLPDNTITRTATSGELASDTVQVQSSNGKNPGRHVSDKDFLFVCGSTFLLEGLASGVKGMAGLGRTRISLPSQFSAEFSFPRKFAVCLSSSTNSKGVVLFGDGPYTFLPNREFANNDFSYTPLFINPVSTASAFSSREPSSEYFIGVKSIKINEKVVPINTTLLSIDNQGVGGTKISTVNPYTILETSIYNAVTNFFVKELVNITRVASVAPFRACFDSRNIASTRVGPAVPSIDLVLQNENVFWRIFGANSMVQVSENVLCLGFVDGGVSPRTSIVVGGYTIEDNLLQFDLARSRLGFTSSILFRQTTCANFNFTSIA
Enzyme Length 439
Uniprot Accession Number P0DO21
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.23.-
Enzyme Function FUNCTION: Involved in plant defense against Phytophtora parasitica (PubMed:28082413). Contributes positively to Nicotiana resistance against P.parasitica (PubMed:28082413). Binds the P.parasitica xyloglucanase XEG1 and inhibits its cell wall degrading enzyme activity and its contribution as P.parasitica virulence factor (PubMed:28082413). XEG1 acts as an important virulence factor during P.parasitica infection but also acts as a pathogen-associated molecular pattern (PAMP) in Nictotiana species, where it can trigger defense responses including cell death (PubMed:28082413). {ECO:0000269|PubMed:28082413}.; FUNCTION: (Microbial infection) Possesses stronger binding affinity with XLP1, a truncated paralog of P.parasitica XEG1 which has no enzyme activity. Is impaired in its inhibitor activity towards the P.parasitica xyloglucanase XEG1 when hijacked by XLP1 binding. {ECO:0000269|PubMed:28082413}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Domain (1); Glycosylation (4); Signal peptide (1)
Keywords Apoplast;Aspartyl protease;Glycoprotein;Hydrolase;Plant defense;Protease;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast {ECO:0000305|PubMed:28082413}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..23; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 47,214
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda