Detail Information for IndEnz0002019032
IED ID IndEnz0002019032
Enzyme Type ID protease019032
Protein Name Ervatamin-C
ERV-C
EC 3.4.22.-
Gene Name
Organism Tabernaemontana divaricata (Crepe jasmine) (Ervatamia coronaria)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Gentianales Apocynaceae Rauvolfioideae Tabernaemontaneae Tabernaemontaninae Tabernaemontana Tabernaemontana divaricata (Crepe jasmine) (Ervatamia coronaria)
Enzyme Sequence LPEQIDWRKKGAVTPVKNQGSCGSCWAFSTVSTVESINQIRTGNLISLSEQELVDCDKKNHGCLGGAFVFAYQYIINNGGIDTQANYPYKAVQGPCQAASKVVSIDGYNGVPFCNEXALKQAVAVQPSTVAIDASSAQFQQYSSGIFSGPCGTKLNHGVTIVGYQANYWIVRNSWGRYWGEKGYIRMLRVGGCGLCGIARLPYYPTKA
Enzyme Length 208
Uniprot Accession Number P83654
Absorption
Active Site ACT_SITE 25; /evidence=ECO:0000250|UniProtKB:P00785; ACT_SITE 157; /evidence=ECO:0000250|UniProtKB:P00785; ACT_SITE 173; /evidence=ECO:0000250|UniProtKB:P00785
Activity Regulation ACTIVITY REGULATION: Activated by thio-specific activators such as cysteine, beta-mercaptoethanol, DTT and glutathione. Inhibited by the thiol-specific inhibitors leupeptin, iodoacetamide, PCMB, NEM, mercuric chloride and sodium tetrathionate. {ECO:0000269|PubMed:9836431}.
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.22.-
Enzyme Function FUNCTION: Cysteine proteinase. Hydrolyzes denatured natural substrates such as casein, hemoglobin, azoalbumin and azocasein with a high specific activity. Has little or no activity against synthetic substrates. {ECO:0000269|PubMed:9836431}.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius with azoalbumin as substrate. Thermostable up to 70 degrees Celsius.;
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.5-8.0 with azoalbumin or hemoglobin as substrate. Active and stable from pH 2.0 to 12.0.;
Pathway
nucleotide Binding
Features Active site (3); Beta strand (8); Chain (1); Disulfide bond (4); Helix (7); Sequence conflict (2); Turn (1)
Keywords 3D-structure;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Secreted;Thiol protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.2, ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (2)
Cross Reference PDB 1O0E; 2PNS;
Mapped Pubmed ID 17767923;
Motif
Gene Encoded By
Mass 22,523
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=9.09 uM for azoalbumin;
Metal Binding
Rhea ID
Cross Reference Brenda 3.4.22.B50;