| IED ID | IndEnz0002019041 |
| Enzyme Type ID | protease019041 |
| Protein Name |
Vacuolar-sorting protein BRO1 Amino acid sensor-independent protein 6 BCK1-like resistance to osmotic shock protein 1 BRO domain-containing protein 1 Nitrogen permease inactivating protein 3 Vacuolar protein-sorting-associated protein 31 |
| Gene Name | BRO1 ASI6 LPF2 NPI3 VPS31 YPL084W |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MKPYLFDLKLKDTEKLDWKKGLSSYLKKSYGSSQWRTFYDEKATSELDHLRNNANGELAPSSLSEQNLKYYSFLEHLYFRLGSKGSRLKMDFTWYDAEYSSAQKGLKYTQHTLAFEKSCTLFNIAVIFTQIARENINEDYKNSIANLTKAFSCFEYLSENFLNSPSVDLQSENTRFLANICHAEAQELFVLKLLNDQISSKQYTLISKLSRATCNLFQKCHDFMKEIDDDVAIYGEPKWKTTVTCKLHFYKSLSAYYHGLHLEEENRVGEAIAFLDFSMQQLISSLPFKTWLVEFIDFDGFKETLEKKQKELIKDNDFIYHESVPAVVQVDSIKALDAIKSPTWEKILEPYMQDVANKCDSLYRGIIPLDVYEKESIYSEEKATLLRKQVEETETANLEYSSFIEFTNLPRLLSDLEKQFSDGNIFSNTDTQGQLMRDQIQTWCKFIQTNEFRDIEEQMNKIVFKRKQILEILSALPNDQKENVTKLKSSLVAASNSDEKLFACVKPHIVEINLLNDNGKIWKKFDEFNRNTPPQPSLLDIDDTKNDKILELLKQVKGHAEDLRTLKEERSRNLSELRDEINNDDITKLLIINKGKSDVELKDLFEVELEKFEPLSTRIEATIYKQSSMIDDIKAKLDEIFHLSNFKDKSSGEEKFLEDRKNFFDKLQEAVKSFSIFASDLPKGIEFYDSLFNMSRDLAERVRVAKQTEDSTANSPAPPLPPLDSKASVVGGPPLLPQKSAAFQSLSRQGLNLGDQFQNLKISAGSDLPQGPGIPPRTYEASPYAATPTMAAPPVPPKQSQEDMYDLRRRKAVENEERELQENPTSFYNRPSVFDENMYSKYSS |
| Enzyme Length | 844 |
| Uniprot Accession Number | P48582 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. Acts as an adapter that recruits the DOA4 deubiquitinase to the endosomes, leading to deubiquitination of cargo proteins prior to the lumenal sequestration. Its association to the endosomes depends on SNF7 and its dissociation requires VPS4. Interacts functionally with the Pkc1p-mitogen-activated protein kinase pathway. {ECO:0000269|PubMed:11454748, ECO:0000269|PubMed:12062418, ECO:0000269|PubMed:12668726, ECO:0000269|PubMed:14523026, ECO:0000269|PubMed:15326198, ECO:0000269|PubMed:8649366}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (6); Chain (1); Coiled coil (1); Compositional bias (2); Domain (1); Helix (17); Modified residue (1); Region (2); Sequence conflict (1); Turn (4) |
| Keywords | 3D-structure;Coiled coil;Cytoplasm;Endosome;Phosphoprotein;Protein transport;Reference proteome;Transport |
| Interact With | P32571; Q08003; P39929 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm. Endosome. |
| Modified Residue | MOD_RES 740; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:18407956 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 1ZB1; |
| Mapped Pubmed ID | 10900456; 11805826; 14690591; 15371534; 15487944; 15569240; 15579677; 15757670; 15935782; 16429126; 16554755; 16689637; 16864574; 17043098; 17145966; 17305427; 17446860; 17513562; 18806212; 19143620; 19143630; 19410548; 19536198; 19841731; 20041173; 20058707; 20190076; 20206679; 20588296; 21035538; 21263029; 21427232; 21430061; 21447998; 21777356; 21824003; 21908597; 22360598; 22388927; 23285107; 23383298; 23444383; 23726974; 23974285; 24148098; 24287454; 24962567; 25303532; 25396681; 25460796; 25462441; 25635693; 26150415; 26427873; 26585826; 26792735; 27074665; 27693354; 27834202; 3062374; 34160559; 9841672; |
| Motif | |
| Gene Encoded By | |
| Mass | 97,276 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |