Detail Information for IndEnz0002019050
IED ID IndEnz0002019050
Enzyme Type ID protease019050
Protein Name Fructose-bisphosphate aldolase A
EC 4.1.2.13
Muscle-type aldolase
Gene Name Aldoa
Organism Rattus norvegicus (Rat)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat)
Enzyme Sequence MPHPYPALTPEQKKELADIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSSLAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHVYLEGTLLKPNMVTPGHACTQKFSNEEIAMATVTALRRTVPPAVPGVTFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYIKRALANSLACQGKYTPSGQSGAAASESLFISNHAY
Enzyme Length 364
Uniprot Accession Number P05065
Absorption
Active Site ACT_SITE 188; /note=Proton acceptor; /evidence=ECO:0000250; ACT_SITE 230; /note=Schiff-base intermediate with dihydroxyacetone-P
Activity Regulation
Binding Site BINDING 56; /note=Substrate; BINDING 147; /note=Substrate
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate; Xref=Rhea:RHEA:14729, ChEBI:CHEBI:32966, ChEBI:CHEBI:57642, ChEBI:CHEBI:59776; EC=4.1.2.13;
DNA Binding
EC Number 4.1.2.13
Enzyme Function FUNCTION: Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
nucleotide Binding
Features Active site (2); Binding site (2); Chain (1); Cross-link (2); Initiator methionine (1); Modified residue (15); Sequence conflict (3); Site (1)
Keywords Acetylation;Cytoplasm;Direct protein sequencing;Glycolysis;Hydroxylation;Isopeptide bond;Lyase;Phosphoprotein;Reference proteome;Schiff base;Ubl conjugation
Interact With Q80Z30
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm, myofibril, sarcomere, I band. Cytoplasm, myofibril, sarcomere, M line. Note=In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca(2+). {ECO:0000250}.
Modified Residue MOD_RES 5; /note=Phosphotyrosine; /evidence=ECO:0007744|PubMed:22673903; MOD_RES 9; /note=Phosphothreonine; /evidence=ECO:0007744|PubMed:22673903; MOD_RES 36; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P04075; MOD_RES 39; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P04075; MOD_RES 42; /note=N6-acetyllysine; alternate; /evidence=ECO:0000250|UniProtKB:P04075; MOD_RES 46; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P04075; MOD_RES 99; /note=N6-(2-hydroxyisobutyryl)lysine; /evidence=ECO:0000250|UniProtKB:P04075; MOD_RES 108; /note=N6-acetyllysine; /evidence=ECO:0000250|UniProtKB:P04075; MOD_RES 111; /note=N6-acetyllysine; alternate; /evidence=ECO:0000250|UniProtKB:P09972; MOD_RES 111; /note=N6-malonyllysine; alternate; /evidence=ECO:0000250; MOD_RES 132; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:22673903; MOD_RES 147; /note=N6-(2-hydroxyisobutyryl)lysine; /evidence=ECO:0000250|UniProtKB:P04075; MOD_RES 272; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P04075; MOD_RES 312; /note=N6-malonyllysine; /evidence=ECO:0000250; MOD_RES 330; /note=N6-acetyllysine; /evidence=ECO:0000250|UniProtKB:P04075
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10395295; 14760703; 15389646; 15680915; 16339744; 16396496; 16622831; 16930621; 18384645; 19081846; 19427860; 19935650; 201371; 21482559; 21890532; 22206666;
Motif
Gene Encoded By
Mass 39,352
Kinetics
Metal Binding
Rhea ID RHEA:14729
Cross Reference Brenda