IED ID | IndEnz0002019053 |
Enzyme Type ID | protease019053 |
Protein Name |
Alkaline serine protease ver112 EC 3.4.21.- |
Gene Name | |
Organism | Lecanicillium psalliotae (Verticillium psalliotae) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Cordycipitaceae Lecanicillium Lecanicillium psalliotae (Verticillium psalliotae) |
Enzyme Sequence | MRLSIIAAVLPLALAAPVAEPEIAPLIEARGAQPIAGKYIVKLKDEAKFGIMNAKSKIPGIERVYENVLNGFSATLSNEELERLRRDPDVESIEQDAIFSINAITQQQGATWGLTRISHRARGSTAYAYDTSAGAGACVYVIDTGVEDTHPDFEGRAKQIKSYASTARDGHGHGTHCAGTIGSKTWGVAKKVSIFGVKVLDDSGSGSLSNIVAGMDFVASDRQSRNCPRRTVASMSLGGGYSAALNQAAARLQSSGVFVAVAAGNDNRDAANTSPASEPTVCTVGATDSNDVRSTFSNYGRVVDIFAPGTSITSTWIGGRTNTISGTSMATPHIAGLAAYLFGLEGGSAGAMCGRIQTLSTKNVLTSIPSGTVNYLAFNGAT |
Enzyme Length | 382 |
Uniprot Accession Number | Q68GV9 |
Absorption | |
Active Site | ACT_SITE 143; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 173; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 328; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
Activity Regulation | ACTIVITY REGULATION: Inhibited by phenylmethylsulfonyl fluoride (PMSF). {ECO:0000269|PubMed:16132863}. |
Binding Site | |
Calcium Binding | |
catalytic Activity | |
DNA Binding | |
EC Number | 3.4.21.- |
Enzyme Function | FUNCTION: Serine protease which can degrade the nematode cuticle. {ECO:0000269|PubMed:16132863}. |
Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 60-70 degrees Celsius. {ECO:0000269|PubMed:16132863}; |
PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 9-10. {ECO:0000269|PubMed:16132863}; |
Pathway | |
nucleotide Binding | |
Features | Active site (3); Beta strand (17); Chain (1); Disulfide bond (2); Domain (2); Helix (10); Propeptide (1); Signal peptide (1); Turn (4) |
Keywords | 3D-structure;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Secreted;Serine protease;Signal;Zymogen |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16132863}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..15; /evidence=ECO:0000255 |
Structure 3D | X-ray crystallography (1) |
Cross Reference PDB | 3F7M; |
Mapped Pubmed ID | 20007510; |
Motif | |
Gene Encoded By | |
Mass | 39,717 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |