Detail Information for IndEnz0002019067
IED ID IndEnz0002019067
Enzyme Type ID protease019067
Protein Name Probable cytosol aminopeptidase
EC 3.4.11.1
Leucine aminopeptidase
LAP
EC 3.4.11.10
Leucyl aminopeptidase
Gene Name pepA BR0689 BS1330_I0685
Organism Brucella suis biovar 1 (strain 1330)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Alphaproteobacteria Hyphomicrobiales Brucellaceae Brucella/Ochrobactrum group Brucella Brucella suis Brucella suis biovar 1 (strain 1330)
Enzyme Sequence MSKRPSISFSEFEVPQKGVAVVLVAKGGGFADEAAKAVGGAEKIARIADISGFTGALGKTAEAIETTPAGVEKIVLVGVGEPGKLGNDDWLKIGGAAFSQIGNAERVTLTLALPETTIAGDEAADVALGMVLRSYKFDRYKTRKSEENGEPKHAAKITICVADTHSARKTFEVAEAVADGVIQARNLVNEPANILGPVEFAEEAEKLEKLGVKVEVLGEKELKKLGMGALLGVAQGSVRPPRLVVMEWHGAKGKEKPIAFVGKGVVFDTGGISIKPAANMEDMKGDMGGAAAVTGLMRALAGRKAKVNAIGVIGLVENMPDGNAQRPGDIVTSMSGQTIEVINTDAEGRLVLADALHYTNDRFKPRFIINLATLTGAVMVALGQYHAGLFSNDDELADQLYDAGQSTGEKLWRLPLGTEYDKMIDSKFADMKNSAGRYGGSITAAQFLKRFVGETPWAHLDVAGTAMGSPANEYNQSWASGFGVRLLDRLVRDQFES
Enzyme Length 497
Uniprot Accession Number Q8G1M4
Absorption
Active Site ACT_SITE 275; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 349; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181};
DNA Binding
EC Number 3.4.11.1; 3.4.11.10
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (7)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 52,576
Kinetics
Metal Binding METAL 263; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 268; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 268; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 286; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 345; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 347; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 347; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Rhea ID
Cross Reference Brenda