Detail Information for IndEnz0002019068
IED ID IndEnz0002019068
Enzyme Type ID protease019068
Protein Name Probable cytosol aminopeptidase
EC 3.4.11.1
Leucine aminopeptidase
LAP
EC 3.4.11.10
Leucyl aminopeptidase
Gene Name pepA BUAP5A_360
Organism Buchnera aphidicola subsp. Acyrthosiphon pisum (strain 5A)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Erwiniaceae Buchnera (aphid P-endosymbionts) Buchnera aphidicola Buchnera aphidicola subsp. Acyrthosiphon pisum (strain 5A)
Enzyme Sequence MNFFIKSCFLDKEKTDCIVVSVFELSELSDSAIYLDKCSNGHITSLIKLGDIQGKIGDTLMLYKVPKILSKRILLVGCGKKDEINIIRFKKILKNTIHAIKKKSIKNIVYSFSNINIDNIYWMIRRMVLSLKESLYETIKINNTNIKNTNIHSITLNIIKKNDLFIAKTALKHALAIDHAITSTKNLSNLPPNICNPLYLSYKAQELSKKYENNIVVEIIDIKKMKELGMNAYIAVGNGSKNKPFMSVIKYSGNNIVNKKIIAFVGKGLTFDSGGISIKPALHMHEMKYDMCGAAAVYGTLIMAAELQLPLTVIGILSGCENMVGSHSFRPGDVLTTMSGQTVEILNTDAEGRLVLCDSLTYLERFSPDIVIDVATLTGACVTALGESVSGLFSNNEELSNQLLHASQETDDKIWSLPLFSEYHKELNSDIADFSNIGRGKAGAITAACFLSKFTKKYNWAHLDIAGTAWKSGKKSGATGRPVELLCQFLLNQSNYIYN
Enzyme Length 499
Uniprot Accession Number B8D9F2
Absorption
Active Site ACT_SITE 279; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 353; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181};
DNA Binding
EC Number 3.4.11.1; 3.4.11.10
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (7)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 55,283
Kinetics
Metal Binding METAL 267; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 272; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 272; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 290; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 349; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 351; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 351; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Rhea ID
Cross Reference Brenda