Detail Information for IndEnz0002019151
IED ID IndEnz0002019151
Enzyme Type ID protease019151
Protein Name Aspartic proteinase 39
EC 3.4.23.-
Gene Name A39 At1g65240 T23K8.15
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MELRRKLCIVVAVFVIVIEFASANFVFKAQHKFAGKKKNLEHFKSHDTRRHSRMLASIDLPLGGDSRVDSVGLYFTKIKLGSPPKEYHVQVDTGSDILWINCKPCPKCPTKTNLNFRLSLFDMNASSTSKKVGCDDDFCSFISQSDSCQPALGCSYHIVYADESTSDGKFIRDMLTLEQVTGDLKTGPLGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTSVLSQLAATGDAKRVFSHCLDNVKGGGIFAVGVVDSPKVKTTPMVPNQMHYNVMLMGMDVDGTSLDLPRSIVRNGGTIVDSGTTLAYFPKVLYDSLIETILARQPVKLHIVEETFQCFSFSTNVDEAFPPVSFEFEDSVKLTVYPHDYLFTLEEELYCFGWQAGGLTTDERSEVILLGDLVLSNKLVVYDLDNEVIGWADHNCSSSIKIKDGSGGVYSVGADNLSSAPRLLMITKLLTILSPLIVMAFTSLA
Enzyme Length 475
Uniprot Accession Number Q9S9K4
Absorption
Active Site ACT_SITE 92; /evidence=ECO:0000255|PROSITE-ProRule:PRU01103; ACT_SITE 303; /evidence=ECO:0000255|PROSITE-ProRule:PRU01103
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.23.-
Enzyme Function FUNCTION: Displays aspartic proteolytic activity (PubMed:27872247). Together with A36, contributes to pollen and ovule development, including the apical cell wall constitution of the growing pollen tubes (PubMed:27872247). {ECO:0000269|PubMed:27872247}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Domain (1); Erroneous gene model prediction (1); Glycosylation (4); Lipidation (1); Mutagenesis (1); Propeptide (1); Signal peptide (1)
Keywords Aspartyl protease;Cell membrane;Cytoplasm;GPI-anchor;Glycoprotein;Hydrolase;Lipoprotein;Membrane;Protease;Reference proteome;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:27872247}; Lipid-anchor, GPI-anchor {ECO:0000255}. Cytoplasm, cytosol {ECO:0000269|PubMed:27872247}. Note=Displays punctate cytosolic localization and colocalizes with the GPI-anchored protein COBL10 at the plasma membrane and in the cytosol (PubMed:27872247). In pollen grains, mainly present at the plasma membrane and in reticular structures in the cytosol (PubMed:27872247). In pollen tubes, exhibits a punctate localization in the cytosol and occurs weakly at the plasma membrane (PubMed:27872247). {ECO:0000269|PubMed:27872247}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..23; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 12068095; 12805588; 14517339; 14671020; 14697270; 16291648; 17317660; 17644812; 18775970;
Motif
Gene Encoded By
Mass 51,828
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda