Detail Information for IndEnz0002019156
IED ID IndEnz0002019156
Enzyme Type ID protease019156
Protein Name Stress activated transcription factor atfs-1
Gene Name atfs-1 ZC376.7
Organism Caenorhabditis elegans
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans
Enzyme Sequence MFSRVGRLTTFGAQAVSNCPFRRDNIYQQPLKVTAPINDQLTSFAHSFSDSVRHRTTSFGNDPFLGVPMDDDEVIKELELLDLDSWHTKPRAPCPAPSDELELDQFWEGKNVTVCGRDPRLGKSTDCFELEAWRPTDSWQNGSSVGHPHGHQQQQQTCQQPPTHSSTTETMHDFSNFGDNMGSPLFQSPSKSAIDQLTGTSRIDEYGMPPQDRKLSKFEMDIEQESKAVDWEAWNHYLESDDDVFKRPEAFFKEEPMIMTSSDSLMTSSTSSPDSGISLYDPMIPPPSSHFPSFNLSSSSSASNLLRLSTPSAPMQQEHRAPVRMHHDVDLFSSGPLLCVPKQEDVFDDFIQQRDDDDEDYIPASEARRTSSRLNRKSATPTYLRRRDSERSWTPASDDYFPEEHQKFKKRGVVLKPSVDEETDRRRMLNRIAAVRYREKKRAEKKGRKMEFQEVADRNRILLQKERQLKREINSMKKELRKMGAIIQ
Enzyme Length 488
Uniprot Accession Number Q23272
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Acts as a transcription factor during mitochondrial stress by activating the mitochondrial unfolded protein response (mtUPR) (PubMed:22700657, PubMed:25274306, PubMed:25773600, PubMed:30642431). Induces nuclear and mitochondrial gene transcription, including genes coding for mitochondrial chaperones and proteins involved in glycolysis, amino acid catabolism and innate immunity (PubMed:22700657, PubMed:25274306, PubMed:25773600). Following mitochondrial stress, restores mitochondrial respiratory capacity by limiting the transcription of oxidative phosphorylation (OXPHOS) machinery genes and by promoting the assembly of OXPHOS complexes via the up-regulation of chaperone and assembly factor genes (PubMed:25773600). Component of a feedback loop involving atfs-1, atgl-1 and hlh-11 (PubMed:33078707). Acts together with flp-7 to negatively regulate the expression of the transcription regulator hlh-11, to promote expression of atgl-1, and thus atgl-1-dependent fat oxidation in response to mitochondrial stress (PubMed:33078707). In addition, functions with hlh-11 to maintain lifespan (PubMed:33078707). Promotes mtDNA maintenance and propagation of deleterious mtDNA (PubMed:27135930). {ECO:0000269|PubMed:22700657, ECO:0000269|PubMed:25274306, ECO:0000269|PubMed:25773600, ECO:0000269|PubMed:27135930, ECO:0000269|PubMed:30642431, ECO:0000269|PubMed:33078707}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Alternative sequence (3); Chain (1); Compositional bias (2); Cross-link (1); Domain (1); Motif (1); Mutagenesis (4); Region (4); Transit peptide (1)
Keywords Alternative splicing;Cytoplasm;DNA-binding;Immunity;Innate immunity;Isopeptide bond;Mitochondrion;Nucleus;Reference proteome;Transcription;Transcription regulation;Transit peptide;Unfolded protein response
Interact With Q8IG69
Induction INDUCTION: [Isoform b]: Induced by mitochondrial stress. {ECO:0000269|PubMed:25773600}.
Subcellular Location SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000269|PubMed:22700657}. Cytoplasm {ECO:0000269|PubMed:22700657}. Nucleus {ECO:0000269|PubMed:22700657, ECO:0000269|PubMed:25274306, ECO:0000269|PubMed:30642431}. Note=In absence of mitochondrial stress, localizes mainly to mitochondria where it is rapidly degraded by protease lonp-1 (PubMed:22700657). Upon mitochondrial stress which causes a reduction in protein mitochondrial import, accumulates in the cytoplasm and translocates into the nucleus to activate transcription of mitochondrial unfolded protein response genes (PubMed:22700657). Similarly, accumulates in the nucleus in response to P.aeruginosa-mediated infection (PubMed:25274306). {ECO:0000269|PubMed:22700657, ECO:0000269|PubMed:25274306}.
Modified Residue
Post Translational Modification PTM: May be desumoylated by ulp-4. {ECO:0000305|PubMed:30642431}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 15990870; 20188671; 20439776; 21085631; 21177967; 21367940; 21938618; 22560298; 22719267; 23516373; 23530189; 23586691; 23661758; 23791784; 23800452; 24407905; 24662282; 24764321; 25487147; 26038366; 26234215; 26913604; 27133166; 27420916; 27610575; 30057120; 30563508; 33229544; 33547005;
Motif MOTIF 436..441; /note="Nuclear localization signal"; /evidence="ECO:0000305|PubMed:22700657, ECO:0000305|PubMed:25274306, ECO:0000305|PubMed:25773600"
Gene Encoded By
Mass 56,019
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda