| IED ID |
IndEnz0002019156 |
| Enzyme Type ID |
protease019156 |
| Protein Name |
Stress activated transcription factor atfs-1
|
| Gene Name |
atfs-1 ZC376.7 |
| Organism |
Caenorhabditis elegans |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Metazoa
Eumetazoa
Bilateria
Protostomia
Ecdysozoa
Nematoda (roundworms)
Chromadorea
Rhabditida
Rhabditina
Rhabditomorpha
Rhabditoidea
Rhabditidae
Peloderinae
Caenorhabditis
Caenorhabditis elegans
|
| Enzyme Sequence |
MFSRVGRLTTFGAQAVSNCPFRRDNIYQQPLKVTAPINDQLTSFAHSFSDSVRHRTTSFGNDPFLGVPMDDDEVIKELELLDLDSWHTKPRAPCPAPSDELELDQFWEGKNVTVCGRDPRLGKSTDCFELEAWRPTDSWQNGSSVGHPHGHQQQQQTCQQPPTHSSTTETMHDFSNFGDNMGSPLFQSPSKSAIDQLTGTSRIDEYGMPPQDRKLSKFEMDIEQESKAVDWEAWNHYLESDDDVFKRPEAFFKEEPMIMTSSDSLMTSSTSSPDSGISLYDPMIPPPSSHFPSFNLSSSSSASNLLRLSTPSAPMQQEHRAPVRMHHDVDLFSSGPLLCVPKQEDVFDDFIQQRDDDDEDYIPASEARRTSSRLNRKSATPTYLRRRDSERSWTPASDDYFPEEHQKFKKRGVVLKPSVDEETDRRRMLNRIAAVRYREKKRAEKKGRKMEFQEVADRNRILLQKERQLKREINSMKKELRKMGAIIQ |
| Enzyme Length |
488 |
| Uniprot Accession Number |
Q23272 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
|
| Enzyme Function |
FUNCTION: Acts as a transcription factor during mitochondrial stress by activating the mitochondrial unfolded protein response (mtUPR) (PubMed:22700657, PubMed:25274306, PubMed:25773600, PubMed:30642431). Induces nuclear and mitochondrial gene transcription, including genes coding for mitochondrial chaperones and proteins involved in glycolysis, amino acid catabolism and innate immunity (PubMed:22700657, PubMed:25274306, PubMed:25773600). Following mitochondrial stress, restores mitochondrial respiratory capacity by limiting the transcription of oxidative phosphorylation (OXPHOS) machinery genes and by promoting the assembly of OXPHOS complexes via the up-regulation of chaperone and assembly factor genes (PubMed:25773600). Component of a feedback loop involving atfs-1, atgl-1 and hlh-11 (PubMed:33078707). Acts together with flp-7 to negatively regulate the expression of the transcription regulator hlh-11, to promote expression of atgl-1, and thus atgl-1-dependent fat oxidation in response to mitochondrial stress (PubMed:33078707). In addition, functions with hlh-11 to maintain lifespan (PubMed:33078707). Promotes mtDNA maintenance and propagation of deleterious mtDNA (PubMed:27135930). {ECO:0000269|PubMed:22700657, ECO:0000269|PubMed:25274306, ECO:0000269|PubMed:25773600, ECO:0000269|PubMed:27135930, ECO:0000269|PubMed:30642431, ECO:0000269|PubMed:33078707}. |
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Alternative sequence (3); Chain (1); Compositional bias (2); Cross-link (1); Domain (1); Motif (1); Mutagenesis (4); Region (4); Transit peptide (1) |
| Keywords |
Alternative splicing;Cytoplasm;DNA-binding;Immunity;Innate immunity;Isopeptide bond;Mitochondrion;Nucleus;Reference proteome;Transcription;Transcription regulation;Transit peptide;Unfolded protein response |
| Interact With |
Q8IG69 |
| Induction |
INDUCTION: [Isoform b]: Induced by mitochondrial stress. {ECO:0000269|PubMed:25773600}. |
| Subcellular Location |
SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000269|PubMed:22700657}. Cytoplasm {ECO:0000269|PubMed:22700657}. Nucleus {ECO:0000269|PubMed:22700657, ECO:0000269|PubMed:25274306, ECO:0000269|PubMed:30642431}. Note=In absence of mitochondrial stress, localizes mainly to mitochondria where it is rapidly degraded by protease lonp-1 (PubMed:22700657). Upon mitochondrial stress which causes a reduction in protein mitochondrial import, accumulates in the cytoplasm and translocates into the nucleus to activate transcription of mitochondrial unfolded protein response genes (PubMed:22700657). Similarly, accumulates in the nucleus in response to P.aeruginosa-mediated infection (PubMed:25274306). {ECO:0000269|PubMed:22700657, ECO:0000269|PubMed:25274306}. |
| Modified Residue |
|
| Post Translational Modification |
PTM: May be desumoylated by ulp-4. {ECO:0000305|PubMed:30642431}. |
| Signal Peptide |
|
| Structure 3D |
|
| Cross Reference PDB |
- |
| Mapped Pubmed ID |
15990870;
20188671;
20439776;
21085631;
21177967;
21367940;
21938618;
22560298;
22719267;
23516373;
23530189;
23586691;
23661758;
23791784;
23800452;
24407905;
24662282;
24764321;
25487147;
26038366;
26234215;
26913604;
27133166;
27420916;
27610575;
30057120;
30563508;
33229544;
33547005;
|
| Motif |
MOTIF 436..441; /note="Nuclear localization signal"; /evidence="ECO:0000305|PubMed:22700657, ECO:0000305|PubMed:25274306, ECO:0000305|PubMed:25773600" |
| Gene Encoded By |
|
| Mass |
56,019 |
| Kinetics |
|
| Metal Binding |
|
| Rhea ID |
|
| Cross Reference Brenda |
|