Detail Information for IndEnz0002019187
IED ID IndEnz0002019187
Enzyme Type ID protease019187
Protein Name Beta-Ala-His dipeptidase
EC 3.4.13.20
CNDP dipeptidase 1
Carnosine dipeptidase 1
Gene Name Cndp1 Cn1
Organism Rattus norvegicus (Rat)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat)
Enzyme Sequence MLSPPHSGTLEKLFQYIDLHQDEFVQTLKEWVAIESDSVQPMPRLRQELFRMMALAADKLRNLGARVDSVDLGSQQMPDGQSLPTPPIILAELGNDPKKPSVCFYGHLDVQPAQKEDGWLTDPYTLTEVDGKLYGRGATDNKGPVLAWINAVSTFRALQQDLPVNVKFILEGMEEAGSVALEELVKREKDNFFSGVDYIVISDNLWLSQKKPALTCGTRGNCYFTVEVKCRDQDFHSGTFGGILNEPMADLVALLGSLVDSSGHILVPGIYDQMAPITEEEKTMYENIDLDLEEYQKSSRVERFLFDTKEELLTHLWRYPSLSIHGIEGAFDEPGTKTVIPGRVLGKFSIRLVPHMTPSVVETQVTQHLEAVFSKRNSFNKMAVSMVLGLQPWTANINGTQYLAARRAIQTVFGVDPDMIQDGSTIPIAKIFQDITQKSVMMLPLGAVDDGEHSQNEKINRWNYIQGSKLFAAFFLELSKLHSGQQVPSGAF
Enzyme Length 492
Uniprot Accession Number Q66HG3
Absorption
Active Site ACT_SITE 109; /evidence=ECO:0000250; ACT_SITE 174; /note=Proton acceptor; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Preferential hydrolysis of the beta-Ala-|-His dipeptide (carnosine), and also anserine, Xaa-|-His dipeptides and other dipeptides including homocarnosine.; EC=3.4.13.20; Evidence={ECO:0000250|UniProtKB:Q8BUG2, ECO:0000250|UniProtKB:Q96KN2}; CATALYTIC ACTIVITY: Reaction=carnosine + H2O = beta-alanine + L-histidine; Xref=Rhea:RHEA:59360, ChEBI:CHEBI:15377, ChEBI:CHEBI:57485, ChEBI:CHEBI:57595, ChEBI:CHEBI:57966; EC=3.4.13.20; Evidence={ECO:0000250|UniProtKB:Q8BUG2, ECO:0000250|UniProtKB:Q96KN2};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59361; Evidence={ECO:0000250|UniProtKB:Q8BUG2}; CATALYTIC ACTIVITY: Reaction=anserine + H2O = beta-alanine + N(pros)-methyl-L-histidine; Xref=Rhea:RHEA:59576, ChEBI:CHEBI:15377, ChEBI:CHEBI:57966, ChEBI:CHEBI:58445, ChEBI:CHEBI:143076; EC=3.4.13.20; Evidence={ECO:0000250|UniProtKB:Q96KN2};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59577; Evidence={ECO:0000250|UniProtKB:Q96KN2}; CATALYTIC ACTIVITY: Reaction=H2O + L-alanyl-L-histidine = L-alanine + L-histidine; Xref=Rhea:RHEA:37283, ChEBI:CHEBI:15377, ChEBI:CHEBI:57595, ChEBI:CHEBI:57972, ChEBI:CHEBI:74388; Evidence={ECO:0000250|UniProtKB:Q96KN2};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37284; Evidence={ECO:0000250|UniProtKB:Q96KN2}; CATALYTIC ACTIVITY: Reaction=glycyl-L-histidine + H2O = glycine + L-histidine; Xref=Rhea:RHEA:67376, ChEBI:CHEBI:15377, ChEBI:CHEBI:57305, ChEBI:CHEBI:57595, ChEBI:CHEBI:169956; Evidence={ECO:0000250|UniProtKB:Q96KN2};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67377; Evidence={ECO:0000250|UniProtKB:Q96KN2}; CATALYTIC ACTIVITY: Reaction=H2O + L-homocarnosine = 4-aminobutanoate + L-histidine; Xref=Rhea:RHEA:59572, ChEBI:CHEBI:15377, ChEBI:CHEBI:57595, ChEBI:CHEBI:59888, ChEBI:CHEBI:143075; EC=3.4.13.20; Evidence={ECO:0000250|UniProtKB:Q96KN2};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59573; Evidence={ECO:0000250|UniProtKB:Q96KN2};
DNA Binding
EC Number 3.4.13.20
Enzyme Function FUNCTION: Catalyzes the peptide bond hydrolysis in Xaa-His dipeptides, displaying the highest activity toward carnosine (beta-alanyl-L-histidine) and anserine (beta-alanyl-3-methyl-histidine). {ECO:0000250|UniProtKB:Q96KN2}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (6); Modified residue (1)
Keywords Carboxypeptidase;Hydrolase;Metal-binding;Metalloprotease;Phosphoprotein;Protease;Reference proteome;Secreted;Zinc
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q96KN2}.
Modified Residue MOD_RES 194; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:16641100
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 54,928
Kinetics
Metal Binding METAL 107; /note=Zinc 2; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:Q96KN2; METAL 140; /note=Zinc 1; /evidence=ECO:0000250|UniProtKB:Q96KN2; METAL 140; /note=Zinc 2; /evidence=ECO:0000250|UniProtKB:Q96KN2; METAL 175; /note=Zinc 1; /evidence=ECO:0000250|UniProtKB:Q96KN2; METAL 203; /note=Zinc 2; /evidence=ECO:0000250|UniProtKB:Q96KN2; METAL 453; /note=Zinc 1; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:Q96KN2
Rhea ID RHEA:59360; RHEA:59361; RHEA:59576; RHEA:59577; RHEA:37283; RHEA:37284; RHEA:67376; RHEA:67377; RHEA:59572; RHEA:59573
Cross Reference Brenda