Detail Information for IndEnz0002019192
IED ID IndEnz0002019192
Enzyme Type ID protease019192
Protein Name Subtilisin-like serine protease AsES
EC 3.4.21.-
Alkaline serine protease AsES
Extra-cellular elicitor protein AsES
Gene Name AsES
Organism Sarocladium strictum (Black bundle disease fungus) (Acremonium strictum)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Sarocladiaceae Sarocladium Sarocladium strictum (Black bundle disease fungus) (Acremonium strictum)
Enzyme Sequence MRLSLVLALLPVAFGAPTRRDEPAPLHVPRDVDSLIKDTYIVKYKDITAFSAVDEGLKLLSGKPKHIYKGAFKGFSGKIDAKTLELLRDDPSVDFIEQDAIVTLAAYTTQASAPWGLARISTRQRGPTGYTYDDSAGAGTCSYIIDTGIQANHPNFGGRAFQLVSYQGSNADGNGHGTHVAGTIGSTTYGVAKRTTLLGVKVLSDSGSGSTSGIIAGINYVVSDSRSRSCPNGSVANMSLGGGYSASLNSAAKSLIDNNIFLAVAAGNENQNAANVSPASEPTVCTVGATTSADAKASFSNYGSGVDIFAPGQSILSTWIGSSTNTISGTSMASPHIAGLAAYLAGLEGFPGAQALCNRIVALATTGVITGLPSGTPNRLAFNGNPSG
Enzyme Length 388
Uniprot Accession Number R4IR27
Absorption
Active Site ACT_SITE 146; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 176; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 331; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240
Activity Regulation ACTIVITY REGULATION: The elicitor proteolytic activity is completely inhibited by PMSF. The activity is also significantly reduced by aprotinin (leading to 37% residual activity), by leupeptin (leading to 54% residual activity), by the ovomucoid trypsin inhibitor (leading to 65% residual activity), and by p-aminobenzamidine (leading to 26% residual activity). {ECO:0000269|PubMed:23530047}.
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.21.-
Enzyme Function FUNCTION: Extracellular elicitor protein that induces a strong defense response in strawberry and confers both local and systemic plant resistance against the fungal pathogen Colletotricum acutatum, the casual agent of anthracnose disease (PubMed:23530047). AsES activates a cascade of defense responses, including calcium influx, oxidative burst, hypersensitive cell-death response (HR), accumulation of autofluorescent compounds, cell-wall reinforcement with callose and lignin deposition, salicylic acid accumulation, and expression of defense-related genes, such as PR1, PG1, MYB30, RBOH-D, RBOH-F, CHI23, and FLS (PubMed:23530047, PubMed:26706064, PubMed:28635519). The oxidative burst consists in a progressive extracellular accumulation of H(2)O(2) that starts immediately after the contact with AsES and is preceded by a rapid and transient cell membrane depolarization. During this phase takes place also a rapid intracellular accumulation of NO at the chloroplasts. After the first extracellular H(2)O(2) production phase, two intracellular H(2)O(2) accumulation events occur, the first 2 hours after induction, and the second 7 hours after induction. AsES also produces a transient increase of ion leakage, and a progressive alkalinization of the extracellular medium (PubMed:26562675). Confers also local and systemic plant resistance against Botrytis cinerea in Arabidopsis thaliana. Systemic, but not local resistance is dependent on the length of exposure to AsES. The protection to B.cinerea is due to the induction of the plant defenses via the salicylic acid, jasmonic acid and ethylene signaling pathways (PubMed:26706064). Exhibits subtilisin-like proteolytic activity which is necessary but not sufficient for its elicitor function in strawberry plants. Probably induces defense by means of proteolysis of one or multiple host proteins that are specific targets of this protease (PubMed:23530047). {ECO:0000269|PubMed:23530047, ECO:0000269|PubMed:26562675, ECO:0000269|PubMed:26706064, ECO:0000269|PubMed:28635519}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (2); Domain (1); Glycosylation (2); Propeptide (1); Signal peptide (1); Site (1)
Keywords Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Protease;Secreted;Serine protease;Signal;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23530047}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..15; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 39,721
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda