| IED ID | IndEnz0002019241 |
| Enzyme Type ID | protease019241 |
| Protein Name |
Virulence metalloprotease EC 3.4.24.- Vibriolysin |
| Gene Name | empA |
| Organism | Vibrio anguillarum (Listonella anguillarum) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Vibrio anguillarum (Listonella anguillarum) |
| Enzyme Sequence | MKKVQRQMKWLFLAASISAALPVSAAKMVQVDDPSLLEQALSMQARSIVPTQNGFQVVKSVTLPNGKVKVRYQQMYHGLPVFNTSVVATQTEKGIGQVYGMLAQQIDSDVVSTSPQVEQKQAVSIALTHYQQQNPSLTSADLVTENERAQLMVRLDENQMAQMVYLVDFFVATNEPARPFFFIDANSGDVLQTWEGLNHAEATGTGPGGNQKTGFYQYGTDFPGLVINKVGNTCSMMNSAVKTVDMKHATSGGSTFSYSCTDASNYNDYKAINGAYSPLNDAHYFGKVVFDMYKDWMNTTPLTFQLTMRVHYDSNYENAFWNGSSMTFGDGQNTFYPLVDINVSAHEVSHGFTEQNSGLVYQNMSGGINEAFSDIAGEAAEFYMKGSVDWVVGSDIFKSSGGLRYFDQPSKDGRSIDHASQYYNGLNVHYSSGVFNRAYYLLANKANWSVRKGFEVFTVANQLYWTANSTFDQGGCGVAKAAQDLGYNKADVVDAFNQVGVNASCGVVPPTENVLEKGKPVIGLQGTRSSEAFYTFTVASSTSAKVSISLGSGDADLYVKAGSKPTTSSWDCRPYKSGNNEQCTISATPGTTYHVMLKGYSNYSGVTLRLD |
| Enzyme Length | 611 |
| Uniprot Accession Number | P43147 |
| Absorption | |
| Active Site | ACT_SITE 347; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; ACT_SITE 429; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Extracellular zinc metalloprotease involved in the virulence mechanism of V.anguillarum. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Metal binding (3); Propeptide (1); Signal peptide (1) |
| Keywords | Calcium;Direct protein sequencing;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Signal;Virulence;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | PTM: Seems to be more extensively processed. |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 66,726 |
| Kinetics | |
| Metal Binding | METAL 346; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 350; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 370; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Rhea ID | |
| Cross Reference Brenda |