Detail Information for IndEnz0002019241
IED ID IndEnz0002019241
Enzyme Type ID protease019241
Protein Name Virulence metalloprotease
EC 3.4.24.-
Vibriolysin
Gene Name empA
Organism Vibrio anguillarum (Listonella anguillarum)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Vibrio anguillarum (Listonella anguillarum)
Enzyme Sequence MKKVQRQMKWLFLAASISAALPVSAAKMVQVDDPSLLEQALSMQARSIVPTQNGFQVVKSVTLPNGKVKVRYQQMYHGLPVFNTSVVATQTEKGIGQVYGMLAQQIDSDVVSTSPQVEQKQAVSIALTHYQQQNPSLTSADLVTENERAQLMVRLDENQMAQMVYLVDFFVATNEPARPFFFIDANSGDVLQTWEGLNHAEATGTGPGGNQKTGFYQYGTDFPGLVINKVGNTCSMMNSAVKTVDMKHATSGGSTFSYSCTDASNYNDYKAINGAYSPLNDAHYFGKVVFDMYKDWMNTTPLTFQLTMRVHYDSNYENAFWNGSSMTFGDGQNTFYPLVDINVSAHEVSHGFTEQNSGLVYQNMSGGINEAFSDIAGEAAEFYMKGSVDWVVGSDIFKSSGGLRYFDQPSKDGRSIDHASQYYNGLNVHYSSGVFNRAYYLLANKANWSVRKGFEVFTVANQLYWTANSTFDQGGCGVAKAAQDLGYNKADVVDAFNQVGVNASCGVVPPTENVLEKGKPVIGLQGTRSSEAFYTFTVASSTSAKVSISLGSGDADLYVKAGSKPTTSSWDCRPYKSGNNEQCTISATPGTTYHVMLKGYSNYSGVTLRLD
Enzyme Length 611
Uniprot Accession Number P43147
Absorption
Active Site ACT_SITE 347; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; ACT_SITE 429; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.24.-
Enzyme Function FUNCTION: Extracellular zinc metalloprotease involved in the virulence mechanism of V.anguillarum.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (3); Propeptide (1); Signal peptide (1)
Keywords Calcium;Direct protein sequencing;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Signal;Virulence;Zinc;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted.
Modified Residue
Post Translational Modification PTM: Seems to be more extensively processed.
Signal Peptide SIGNAL 1..25; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 66,726
Kinetics
Metal Binding METAL 346; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 350; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 370; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095
Rhea ID
Cross Reference Brenda