| IED ID | IndEnz0002019289 |
| Enzyme Type ID | protease019289 |
| Protein Name |
C-terminal processing peptidase, chloroplastic EC 3.4.21.102 D1 C-terminal processing protease Photosystem II D1 protein processing peptidase |
| Gene Name | ctpA D1P |
| Organism | Tetradesmus obliquus (Green alga) (Acutodesmus obliquus) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Chlorophyta core chlorophytes Chlorophyceae CS clade Sphaeropleales Scenedesmaceae Tetradesmus Tetradesmus obliquus (Green alga) (Acutodesmus obliquus) |
| Enzyme Sequence | MHSRTNCLQTSVRAPQPHFRPFTAVKTCRQRCSTTAAAAKRDQAQEQQPWIQVGLGLAAAATAVAVGLGAAALPAQAVTSEQLLFLEAWRAVDRAYVDKSFNGQSWFKLRETYLKKEPMDRRAQTYDAIRKLLAVLDDPFTRFLEPSRLAALRRGTAGSVTGVGLEITYDGGSGKDVVVLTPAPGGPAEKAGARAGDVIVTVDGTAVKGLSLYDVSDLLQGEADSQVEVVLHAPGAPSNTRTLQLTRQKVTINPVTFTTCSNVAAAALPPGAAKQQLGYVRLATFNSNTTAAAQQAFTELSKQGVAGLVLDIRNNGGGLFPAGVNVARMLVDRGDLVLIADSQGIRDIYSADGNSIDSATPLVVLVNRGTASASEVLAGALKDSKRGLIAGERTFGKGLIQTVVDLSDGSGVAVTVARYQTPAGVDINKIGVSPDVQLDPEVLPTDLEGVCRVLGSDAAPRLFG |
| Enzyme Length | 464 |
| Uniprot Accession Number | O04073 |
| Absorption | |
| Active Site | ACT_SITE 372; /note=Charge relay system; /evidence=ECO:0000269|PubMed:10966643; ACT_SITE 397; /note=Charge relay system; /evidence=ECO:0000269|PubMed:10966643 |
| Activity Regulation | ACTIVITY REGULATION: Not inhibited by antipain, 4-amidinophenylmethanesulfonyl fluoride, aprotinin, chymostatin, 3,4-dichloroisocoumarin, diisopropyl fluorophosphate, E64, EDTA, EGTA, iodoacetamide, leupeptin, pepstatin, o-phenanthroline, N-ethylmaleimide, phosphoramidon or phenylmethylsulfonyl fluoride. {ECO:0000269|PubMed:9252339}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=The enzyme shows specific recognition of a C-terminal tripeptide, Xaa-Yaa-Zaa, in which Xaa is preferably Ala or Leu, Yaa is preferably Ala or Tyr, and Zaa is preferably Ala, but then cleaves at a variable distance from the C-terminus. A typical cleavage is -Ala-Ala-|-Arg-Ala-Ala-Lys-Glu-Asn-Tyr-Ala-Leu-Ala-Ala.; EC=3.4.21.102; Evidence={ECO:0000303|PubMed:9252339}; |
| DNA Binding | |
| EC Number | 3.4.21.102 |
| Enzyme Function | FUNCTION: Protease involved in the C-terminal processing of the chloroplastic D1 protein of photosystem II. This proteolytic processing is necessary to allow the light-driven assembly of the tetranuclear manganese cluster, which is responsible for photosynthetic water oxidation. {ECO:0000269|PubMed:9252339}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.2. {ECO:0000269|PubMed:9252339}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (20); Chain (1); Domain (1); Helix (12); Transit peptide (2); Turn (1) |
| Keywords | 3D-structure;Chloroplast;Direct protein sequencing;Hydrolase;Plastid;Protease;Serine protease;Thylakoid;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen {ECO:0000303|PubMed:9252339}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 1FC6; 1FC7; 1FC9; 1FCF; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 48,615 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |