| IED ID | IndEnz0002019295 |
| Enzyme Type ID | protease019295 |
| Protein Name |
Protein EDS1 Enhanced disease susceptibility 1 |
| Gene Name | EDS1 EDS1-90 EDS1A At3g48090 T17F15.40 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAFEALTGINGDLITRSWSASKQAYLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSSFGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFVNFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTRVMRDTSTVANQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTSQASDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDGENSIESTLNDLGVSTRGRQYVQAALEEEKKRVENQKKIIQVIEQERFLKKLAWIEDEYKPKCQAHKNGYYDSFKVSNEENDFKANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNSGSECGSCFWAEVEELKGKPYEEVEVRVKTLEGMLGEWITDGEVDDKEIFLEGSTFRKWWITLPKNHKSHSPLRDYMMDEITDT |
| Enzyme Length | 623 |
| Uniprot Accession Number | Q9SU72 |
| Absorption | |
| Active Site | ACT_SITE 123; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P19515; ACT_SITE 187; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P19515; ACT_SITE 317; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P19515 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Positive regulator of basal resistance and of effector-triggered immunity specifically mediated by TIR-NB-LRR (TNL) resistance proteins. Disruption by bacterial effector of EDS1-TIR-NB-LRR resistance protein interactions constitutes the first step in resistance activation (PubMed:22158819). Acts redundantly with salicylic acid to regulate resistance gene-mediated signaling (PubMed:19578402). Triggers early plant defenses and hypersensitive response independently of PAD4, and then recruits PAD4 to potentiate plant defenses through the accumulation of salicylic acid (PubMed:11574472). Nuclear localization is essential for basal and TNL-conditioned immunity and for reprogramming defense gene expression, while cytoplasmic EDS1 is required to induce a complete immune response (PubMed:20617163). Heterodimerization with PAD4 and/or SGA101 is necessary for TNL-mediated effector-triggered immunity (PubMed:24331460). Contributes to nonhost resistance against E.amylovora (PubMed:22316300). Loss of EDS1-PAD4 interaction compromises basal but not TNL-triggered resistance (PubMed:21434927). Necessary for systemic acquired resistance (SAR) signal generation and perception (PubMed:24755512). Has no direct lipase activity (PubMed:16040633). Putative lipase activity is dispensable for immune functions (PubMed:24331460). {ECO:0000269|PubMed:10077677, ECO:0000269|PubMed:11574472, ECO:0000269|PubMed:19578402, ECO:0000269|PubMed:20617163, ECO:0000269|PubMed:21434927, ECO:0000269|PubMed:22158819, ECO:0000269|PubMed:22316300, ECO:0000269|PubMed:24755512, ECO:0000305|PubMed:16040633}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (1); Chain (1); Coiled coil (1); Initiator methionine (1); Modified residue (1); Mutagenesis (2) |
| Keywords | Acetylation;Alternative splicing;Coiled coil;Cytoplasm;Endoplasmic reticulum;Hydrolase;Microsome;Nucleus;Plant defense;Reference proteome |
| Interact With | Q94KL5; Q05466; Q8RXD6; Q9S745; Q38845; Q9S7W5; Q9C9L2 |
| Induction | INDUCTION: Up-regulated by salicylic acid or upon turnip crinkle virus or avirulent bacterial pathogen infection. {ECO:0000269|PubMed:10077677, ECO:0000269|PubMed:22072959}. |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16040633, ECO:0000269|PubMed:22072959, ECO:0000269|PubMed:22158819}. Cytoplasm {ECO:0000269|PubMed:16040633, ECO:0000269|PubMed:22072959, ECO:0000269|PubMed:22158818, ECO:0000269|PubMed:22158819}. Microsome {ECO:0000269|PubMed:22158819}. Note=Found in both the nucleus and diffuse in the cytosol when associated with PAD4, in the nucleus and in punctate spots in the cytoplasm when interacting with SRFR1, SNC1 or RPS4, only in the nucleus when associated with SAG101 and in the cytosol when it dimerizes. Found in the nucleus when interacting with VICTR and PAD4. Interacts with AvrRps4 and HopA1 effectors in microsomes. {ECO:0000269|PubMed:16040633, ECO:0000269|PubMed:22072959, ECO:0000269|PubMed:22158819, ECO:0000269|PubMed:23275581}. |
| Modified Residue | MOD_RES 2; /note=N-acetylalanine; /evidence=ECO:0007744|PubMed:22223895 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10066602; 10571887; 10886772; 11114160; 11277440; 11387977; 11402173; 11439128; 11595797; 11605952; 11826312; 11846877; 11850411; 11874570; 12468733; 12609040; 12609045; 12744510; 12795697; 12971598; 14756307; 15728340; 15773856; 15894742; 15939664; 15986927; 16255244; 16306139; 16367962; 16461580; 16531493; 16790029; 16813576; 16845481; 16889647; 17163880; 17217460; 17419843; 17431038; 17631528; 17725549; 17988883; 18055613; 18065690; 18251883; 18266921; 18567827; 18624640; 18650403; 18705666; 18790826; 18842091; 19054359; 19122675; 19140951; 19348568; 19450522; 19525323; 19549129; 19682297; 19686535; 19694953; 19700630; 19825557; 20145949; 20163553; 20444230; 20447277; 20456049; 20538606; 20636323; 20699401; 21030507; 21143673; 21301097; 21327081; 21615571; 21617027; 21620700; 21798944; 21900271; 22080599; 22112448; 22286136; 22288649; 22299054; 22353573; 22359427; 22408091; 22750992; 22902705; 22990443; 23004358; 23073694; 23264520; 23400705; 23604702; 23617415; 23617639; 23633962; 23651299; 23959884; 24146667; 24372773; 24471507; 24570021; 24723397; 24758581; 24834923; 24855660; 24872380; 25114016; 25303634; 25483988; 25681297; 25727690; 25915452; 25998906; 25999965; 26139525; 26149542; 26225489; 26310916; 26340231; 26493993; 26505534; 26724785; 26750751; 26751786; 26754794; 26973658; 26973671; 27082651; 27219122; 27412712; 27861989; 28018374; 28152090; 28351986; 28475615; 28487714; 28627464; 29253890; 29483147; 29842929; 29912376; 29931201; 30185442; 30461017; 30485803; 30730076; 30763614; 30770836; 31186426; 31311833; 31382023; 31426325; 31449356; 31550533; 31702436; 31876224; 32086363; 32275065; 32396762; 32409319; 32839979; 32937656; 33142885; 34099661; 8953768; 9707643; |
| Motif | |
| Gene Encoded By | |
| Mass | 71,690 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |