| IED ID | IndEnz0002019296 |
| Enzyme Type ID | protease019296 |
| Protein Name |
EEF1AKMT4-ECE2 readthrough transcript protein EC 3.4.24.71 Includes: Methyltransferase-like region EC 2.1.1.- ; Endothelin-converting enzyme 2 region EC 3.4.24.71 |
| Gene Name | EEF1AKMT4-ECE2 |
| Organism | Bos taurus (Bovine) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Ruminantia Pecora Bovidae Bovinae Bos (oxen cattle) Bos taurus (Bovine) |
| Enzyme Sequence | MACLGPSAQVPELPEKNCGYREVQYWDQRYQGAADSAPYEWFGDFSCFRDLLEPELRPLDRILVLGCGNSALSYEIFLGGFPDVTSVDYSSVVVAAMRARYAHVPTLRWETMDVRALGFPSGSFDVVLEKGTLDALLTGEQDPWTVSSEGVHTVDQVLNEAGFRKRTSRLLGLHTQLELVLAGVSLLLAALLLGCLVALGVQYHRDPSHSTCLTEACIRVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAERKTQRFYLSCLQVERIEELGAQPLRDLIDKIGGWNVTGPWDQDNFMEVLKAVAGTYRATPFFTVYVSADSKSSNSNIIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGMLLGGQPTSTREQMRQVLELEIQLANITVPQDQRRDEEKIYHKMSIAELQALAPSMDWLEFLSFLLSPLELGDSEPVVVYGTDYLQQVSELINRTEPSVLNNYLIWNLVQKTTSSLDHRFESAQEKLLETLYGTKKSCTPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMISEIRAAFEEALGHLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEVSEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYTCNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACIEEQYSQYQVNGEKLNGRQTLGENIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNSGQLCEVW |
| Enzyme Length | 883 |
| Uniprot Accession Number | P0DPE2 |
| Absorption | |
| Active Site | ACT_SITE 721; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000255|PROSITE-ProRule:PRU10095"; ACT_SITE 784; /note="Proton donor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233" |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by phosphoramidon. {ECO:0000269|PubMed:7797512}. |
| Binding Site | BINDING 26; /note=S-adenosyl-L-methionine; /evidence=ECO:0000250|UniProtKB:P0DPD7; BINDING 30; /note=S-adenosyl-L-methionine; /evidence=ECO:0000250|UniProtKB:P0DPD7; BINDING 41; /note=S-adenosyl-L-methionine; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:P0DPD7; BINDING 66; /note=S-adenosyl-L-methionine; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P0DPD7; BINDING 130; /note=S-adenosyl-L-methionine; /evidence=ECO:0000250|UniProtKB:P0DPD7 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of the 21-Trp-|-Val-22 bond in big endothelin to form endothelin 1.; EC=3.4.24.71; Evidence={ECO:0000269|PubMed:7797512}; |
| DNA Binding | |
| EC Number | 3.4.24.71; 2.1.1.-; 3.4.24.71 |
| Enzyme Function | FUNCTION: Converts big endothelin-1 to endothelin-1 (PubMed:7797512). May also have methyltransferase activity (Probable). {ECO:0000269|PubMed:7797512, ECO:0000305}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.5. Inactive at neutral pH. {ECO:0000269|PubMed:7797512}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Binding site (5); Chain (1); Disulfide bond (5); Domain (1); Erroneous initiation (3); Frameshift (3); Glycosylation (9); Metal binding (3); Modified residue (1); Region (3); Sequence conflict (15); Topological domain (2); Transmembrane (1) |
| Keywords | Alternative splicing;Cytoplasmic vesicle;Disulfide bond;Glycoprotein;Golgi apparatus;Hydrolase;Membrane;Metal-binding;Metalloprotease;Methyltransferase;Multifunctional enzyme;Phosphoprotein;Protease;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000269|PubMed:12054617}; Single-pass type II membrane protein {ECO:0000305}. Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000269|PubMed:12054617}. |
| Modified Residue | MOD_RES 39; /note=Phosphotyrosine; /evidence=ECO:0000250|UniProtKB:P0DPD8 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 25370848; |
| Motif | |
| Gene Encoded By | |
| Mass | 99,637 |
| Kinetics | |
| Metal Binding | METAL 720; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000255|PROSITE-ProRule:PRU10095"; METAL 724; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000255|PROSITE-ProRule:PRU10095"; METAL 780; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233" |
| Rhea ID | |
| Cross Reference Brenda |