Detail Information for IndEnz0002019296
IED ID IndEnz0002019296
Enzyme Type ID protease019296
Protein Name EEF1AKMT4-ECE2 readthrough transcript protein
EC 3.4.24.71

Includes: Methyltransferase-like region
EC 2.1.1.-
; Endothelin-converting enzyme 2 region
EC 3.4.24.71
Gene Name EEF1AKMT4-ECE2
Organism Bos taurus (Bovine)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Ruminantia Pecora Bovidae Bovinae Bos (oxen cattle) Bos taurus (Bovine)
Enzyme Sequence MACLGPSAQVPELPEKNCGYREVQYWDQRYQGAADSAPYEWFGDFSCFRDLLEPELRPLDRILVLGCGNSALSYEIFLGGFPDVTSVDYSSVVVAAMRARYAHVPTLRWETMDVRALGFPSGSFDVVLEKGTLDALLTGEQDPWTVSSEGVHTVDQVLNEAGFRKRTSRLLGLHTQLELVLAGVSLLLAALLLGCLVALGVQYHRDPSHSTCLTEACIRVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAERKTQRFYLSCLQVERIEELGAQPLRDLIDKIGGWNVTGPWDQDNFMEVLKAVAGTYRATPFFTVYVSADSKSSNSNIIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGMLLGGQPTSTREQMRQVLELEIQLANITVPQDQRRDEEKIYHKMSIAELQALAPSMDWLEFLSFLLSPLELGDSEPVVVYGTDYLQQVSELINRTEPSVLNNYLIWNLVQKTTSSLDHRFESAQEKLLETLYGTKKSCTPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMISEIRAAFEEALGHLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEVSEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYTCNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACIEEQYSQYQVNGEKLNGRQTLGENIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNSGQLCEVW
Enzyme Length 883
Uniprot Accession Number P0DPE2
Absorption
Active Site ACT_SITE 721; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000255|PROSITE-ProRule:PRU10095"; ACT_SITE 784; /note="Proton donor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233"
Activity Regulation ACTIVITY REGULATION: Inhibited by phosphoramidon. {ECO:0000269|PubMed:7797512}.
Binding Site BINDING 26; /note=S-adenosyl-L-methionine; /evidence=ECO:0000250|UniProtKB:P0DPD7; BINDING 30; /note=S-adenosyl-L-methionine; /evidence=ECO:0000250|UniProtKB:P0DPD7; BINDING 41; /note=S-adenosyl-L-methionine; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:P0DPD7; BINDING 66; /note=S-adenosyl-L-methionine; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P0DPD7; BINDING 130; /note=S-adenosyl-L-methionine; /evidence=ECO:0000250|UniProtKB:P0DPD7
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of the 21-Trp-|-Val-22 bond in big endothelin to form endothelin 1.; EC=3.4.24.71; Evidence={ECO:0000269|PubMed:7797512};
DNA Binding
EC Number 3.4.24.71; 2.1.1.-; 3.4.24.71
Enzyme Function FUNCTION: Converts big endothelin-1 to endothelin-1 (PubMed:7797512). May also have methyltransferase activity (Probable). {ECO:0000269|PubMed:7797512, ECO:0000305}.
Temperature Dependency
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.5. Inactive at neutral pH. {ECO:0000269|PubMed:7797512};
Pathway
nucleotide Binding
Features Active site (2); Alternative sequence (1); Binding site (5); Chain (1); Disulfide bond (5); Domain (1); Erroneous initiation (3); Frameshift (3); Glycosylation (9); Metal binding (3); Modified residue (1); Region (3); Sequence conflict (15); Topological domain (2); Transmembrane (1)
Keywords Alternative splicing;Cytoplasmic vesicle;Disulfide bond;Glycoprotein;Golgi apparatus;Hydrolase;Membrane;Metal-binding;Metalloprotease;Methyltransferase;Multifunctional enzyme;Phosphoprotein;Protease;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix;Zinc
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000269|PubMed:12054617}; Single-pass type II membrane protein {ECO:0000305}. Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000269|PubMed:12054617}.
Modified Residue MOD_RES 39; /note=Phosphotyrosine; /evidence=ECO:0000250|UniProtKB:P0DPD8
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 25370848;
Motif
Gene Encoded By
Mass 99,637
Kinetics
Metal Binding METAL 720; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000255|PROSITE-ProRule:PRU10095"; METAL 724; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000255|PROSITE-ProRule:PRU10095"; METAL 780; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233"
Rhea ID
Cross Reference Brenda