| IED ID | IndEnz0002019298 |
| Enzyme Type ID | protease019298 |
| Protein Name |
EEF1AKMT4-ECE2 readthrough transcript protein EC 3.4.24.71 Includes: Methyltransferase-like region EC 2.1.1.- ; Endothelin-converting enzyme 2 region EC 3.4.24.71 |
| Gene Name | Eef1akmt4-Ece2 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MASPRTPVSPPELPEKNFQYRQVQYWDQRYKDAADSGPYEWFGDFASFRALLEPELCPEDRILVLGCGNSALSYELFLGGFPNVTSVDYSPVVVAAMQVRYAHVPSLRWETMDVRALDFPSGSFDVVLEKGTLDAMLAGEPDPWNVSSEGVHTVDQVLSEVGFQKRTRQLFGSHTQLELVLAGLILVLAALLLGCLVALWVHRDPAHSTCVTEACIRVAGKILESLDRGVSPCQDFYQFSCGGWIRRNPLPNGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAERKTRSFYLSCLQSERIEKLGAKPLRDLIDKIGGWNITGPWDEDSFMDVLKAVAGTYRATPFFTVYVSADSKSSNSNIIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMVELGVLLGGQPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPAVDWLEFLSFLLSPLELGDSEPVVVYGTEYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDQRFETAQEKLLETLYGTKKSCTPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMINEIRSAFEETLGDLVWMDEKTRLAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEVSEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYAHNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLTAFQNHTACMEEQYSQYQVNGERLNGLQTLGENIADNGGLKAAYNAYKAWLRKHGEEQPLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW |
| Enzyme Length | 881 |
| Uniprot Accession Number | P0DPD9 |
| Absorption | |
| Active Site | ACT_SITE 719; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000255|PROSITE-ProRule:PRU10095"; ACT_SITE 782; /note="Proton donor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233" |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by phosphoramidon. {ECO:0000250|UniProtKB:P0DPE2}. |
| Binding Site | BINDING 26; /note=S-adenosyl-L-methionine; /evidence=ECO:0000250|UniProtKB:P0DPD7; BINDING 30; /note=S-adenosyl-L-methionine; /evidence=ECO:0000250|UniProtKB:P0DPD7; BINDING 41; /note=S-adenosyl-L-methionine; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:P0DPD7; BINDING 66; /note=S-adenosyl-L-methionine; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P0DPD7; BINDING 130; /note=S-adenosyl-L-methionine; /evidence=ECO:0000250|UniProtKB:P0DPD7 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of the 21-Trp-|-Val-22 bond in big endothelin to form endothelin 1.; EC=3.4.24.71; Evidence={ECO:0000250|UniProtKB:P0DPE2}; |
| DNA Binding | |
| EC Number | 3.4.24.71; 2.1.1.-; 3.4.24.71 |
| Enzyme Function | FUNCTION: Converts big endothelin-1 to endothelin-1. May also have methyltransferase activity (By similarity). May play a role in amyloid-beta processing (PubMed:12464614). {ECO:0000250|UniProtKB:P0DPE2, ECO:0000269|PubMed:12464614}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Binding site (5); Chain (1); Disulfide bond (5); Domain (1); Erroneous initiation (2); Glycosylation (9); Metal binding (3); Modified residue (2); Region (3); Sequence conflict (10); Topological domain (2); Transmembrane (1) |
| Keywords | Alternative splicing;Cytoplasmic vesicle;Disulfide bond;Glycoprotein;Golgi apparatus;Hydrolase;Membrane;Metal-binding;Metalloprotease;Methyltransferase;Multifunctional enzyme;Phosphoprotein;Protease;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250|UniProtKB:P0DPE2}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P0DPE2}. Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000250|UniProtKB:P0DPE2}. |
| Modified Residue | MOD_RES 39; /note=Phosphotyrosine; /evidence=ECO:0000250|UniProtKB:P0DPD8; MOD_RES P0DPD9-2:174; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:21183079 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 20807771; 21450041; 21972895; 24847082; 24990314; 27644077; |
| Motif | |
| Gene Encoded By | |
| Mass | 99,480 |
| Kinetics | |
| Metal Binding | METAL 718; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000255|PROSITE-ProRule:PRU10095"; METAL 722; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000255|PROSITE-ProRule:PRU10095"; METAL 778; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233" |
| Rhea ID | |
| Cross Reference Brenda |