IED ID | IndEnz0002019322 |
Enzyme Type ID | protease019322 |
Protein Name |
Sphingolipid delta 4 -desaturase DES1-like EC 1.14.19.17 |
Gene Name | At4g04930 T1J1.1 |
Organism | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
Enzyme Sequence | MGKGGREKISSNEEEREGVMATDFFWSYTDEPHASRRRQILSCYPQIRQLFGPDPWAFLKITLVVILQLSTAAILHNSGWLKILSIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRCLGIFANLPIGVPMSVTFQKYHLEHHRFQGVDGIDMDVPTYTEAHLVTNIFAKTIWVFLQLFFYALRPIFIKPKPPGYWEFINFLIQIVLDVSVVLFFGWRSFAYLILSTFVGGGMHPMAGHFISEHYVFNPNQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLHLVKEIAGEYYEGLESYKSWSQVIYMYIMDTTVGPYSRMKRKLSKSD |
Enzyme Length | 332 |
Uniprot Accession Number | Q9ZPH4 |
Absorption | |
Active Site | |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=an N-acylsphinganine + 2 Fe(II)-[cytochrome b5] + 2 H(+) + O2 = an N-acylsphing-4-enine + 2 Fe(III)-[cytochrome b5] + 2 H2O; Xref=Rhea:RHEA:46544, Rhea:RHEA-COMP:10438, Rhea:RHEA-COMP:10439, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:29033, ChEBI:CHEBI:29034, ChEBI:CHEBI:31488, ChEBI:CHEBI:52639; EC=1.14.19.17; Evidence={ECO:0000305|PubMed:18978071}; |
DNA Binding | |
EC Number | 1.14.19.17 |
Enzyme Function | FUNCTION: Sphingolipid-delta-4-desaturase required for the biosynthesis of delta-4-unsaturated sphingolipids and derivatives. May be required for the biosynthesis of glucosylceramides. {ECO:0000269|PubMed:18978071}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Chain (1); Motif (3); Transmembrane (6) |
Keywords | Endoplasmic reticulum;Lipid metabolism;Membrane;Oxidoreductase;Reference proteome;Sphingolipid metabolism;Transmembrane;Transmembrane helix |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | 15037448; 15596101; 17346262; 18315867; 18775970; 23505340; 27768815; |
Motif | MOTIF 103..107; /note=Histidine box-1; /evidence=ECO:0000305; MOTIF 140..144; /note=Histidine box-2; /evidence=ECO:0000305; MOTIF 271..275; /note=Histidine box-3; /evidence=ECO:0000305 |
Gene Encoded By | |
Mass | 38,507 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:46544 |
Cross Reference Brenda |