Detail Information for IndEnz0002019334
IED ID IndEnz0002019334
Enzyme Type ID protease019334
Protein Name Fibulin-1
Gene Name fbl-1 fbln1 F56H11.1
Organism Caenorhabditis elegans
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans
Enzyme Sequence MRICFLLLAFLVAETFANELTRCCAGGTRHFKNSNTCSSIKSEGTSMTCQRAASICCLRSLLDNACDSGTDIAKEEESCPSNINILGGGLKKECCDCCLLAKDLLNRNEPCVAPVGFSAGCLRSFNKCCNGDIEITHASEIITGRPLNDPHVLHLGDRCASSHCEHLCHDRGGEKVECSCRSGFDLAPDGMACVDRNECLTRQSPCTQSEDCVNTIGGYICQRRISRLVPHRHRANRIGNAPRRMRDDPYSRAGEYREASQANTEFGCPMGWLFQHGHCVDVDECNLGSHDCGPLYQCRNTQGSYRCDAKKCGDGELQNPMTGECTSITCPNGYYPKNGMCNDIDECVTGHNCGAGEECVNTPGSFRCQQKGNLCAHGYEVNGATGFCEDVNECQQGVCGSMECINLPGTYKCKCGPGYEFNDAKKRCEDVDECIKFAGHVCDLSAECINTIGSFECKCKPGFQLASDGRRCEDVNECTTGIAACEQKCVNIPGSYQCICDRGFALGPDGTKCEDIDECSIWAGSGNDLCMGGCINTKGSYLCQCPPGYKIQPDGRTCVDVDECAMGECAGSDKVCVNTLGSFKCHSIDCPTNYIHDSLNKNQIADGYSCIKVCSTEDTECLGNHTREVLYQFRAVPSLKTIISPIEVSRIVTHMGVPFSVDYNLDYVGQRHFRIVQERNIGIVQLVKPISGPTVETIKVNIHTKSRTGVILAFNEAIIEISVSKYPF
Enzyme Length 728
Uniprot Accession Number O77469
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Incorporated into fibronectin-containing matrix fibers. Plays a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Important for certain developmental processes and contributes to the supramolecular organization of ECM architecture, in particular to those of basement membranes. {ECO:0000269|PubMed:15556862, ECO:0000269|PubMed:15556863, ECO:0000269|PubMed:16120639, ECO:0000269|PubMed:17043142, ECO:0000269|PubMed:19104038, ECO:0000269|PubMed:22298704}.; FUNCTION: [Isoform a]: Involved in regulating the shape and adhesion of cells in the developing pharynx, intestine, body-wall muscle and gonadal tissue (PubMed:16120639). During gonadogenesis, regulates the width of gonads and the migration of distal tip cells (DTC) (PubMed:15556862, PubMed:17043142, PubMed:22298704). Together with type IV collagen let-2 and downstream of metalloprotease mig-17, recruits nidogen nid-1 to the gonad basement membrane thereby inducing basement membrane remodeling required for the directional migration of DTCs (PubMed:15556863, PubMed:19104038). Acts antagonistically with metalloprotease gon-1 to maintain optimal levels of type IV collagen emb-9 in the gonad basement membrane during gonadogenesis (PubMed:15556862, PubMed:17043142, PubMed:22298704). Required for larval development (PubMed:17043142). {ECO:0000269|PubMed:15556862, ECO:0000269|PubMed:15556863, ECO:0000269|PubMed:16120639, ECO:0000269|PubMed:17043142, ECO:0000269|PubMed:19104038}.; FUNCTION: [Isoform c]: Involved in the assembly of the flexible hemicentin-containing tracks found joining the pharynx and body-wall-muscle basement membranes. {ECO:0000269|PubMed:16120639}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Alternative sequence (2); Chain (1); Disulfide bond (30); Domain (12); Glycosylation (1); Mutagenesis (2); Sequence conflict (1); Signal peptide (1)
Keywords Alternative splicing;Basement membrane;Calcium;Developmental protein;Disulfide bond;EGF-like domain;Extracellular matrix;Glycoprotein;Reference proteome;Repeat;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: [Isoform a]: Secreted, extracellular space, extracellular matrix, basement membrane {ECO:0000269|PubMed:15556862, ECO:0000269|PubMed:15556863, ECO:0000269|PubMed:17043142}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..17; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10615043; 12679813; 14704431; 15338614; 17164286; 17486083; 17850180; 18692475; 19343510; 21110867; 21177967; 21367940; 22051349; 22347378; 22560298; 22981695; 23549480; 23800452; 24318535; 25487147; 26539892;
Motif
Gene Encoded By
Mass 79,321
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda