Detail Information for IndEnz0002019343
IED ID IndEnz0002019343
Enzyme Type ID protease019343
Protein Name DNA-binding transcriptional activator AlpA
Prophage CP4-57 regulatory protein AlpA
Gene Name alpA alp b2624 JW2604
Organism Escherichia coli (strain K12)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12)
Enzyme Sequence MNNRSAVRILRLPAVIQKTGMARATIYDWLNPKSPRYDATFPKKRMLGVKSVGWIEAEIDEWLSQRCKLI
Enzyme Length 70
Uniprot Accession Number P33997
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding DNA_BIND 12..31; /note=H-T-H motif; /evidence=ECO:0000255
EC Number
Enzyme Function FUNCTION: Positive regulator of the expression of the slpA gene (PubMed:7511582). When overexpressed, leads to suppression of the capsule overproduction and UV sensitivity phenotypes of cells mutant for the Lon ATP-dependent protease (PubMed:7511582). Part of the cryptic P4-like prophage CP4-57 (PubMed:7511583). Overexpression of AlpA leads to excision of the CP4-57 prophage by IntA. This inactivates ssrA (the gene upstream of the prophage) that encodes tmRNA which is required to rescue stalled ribosomes in a process known as trans-translation (PubMed:7511583). {ECO:0000269|PubMed:7511582, ECO:0000269|PubMed:7511583}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); DNA binding (1)
Keywords Activator;DNA-binding;Reference proteome;Transcription;Transcription regulation
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 16606699;
Motif
Gene Encoded By
Mass 8,133
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda