Detail Information for IndEnz0002019346
IED ID IndEnz0002019346
Enzyme Type ID protease019346
Protein Name Alkaline proteinase
ALP
EC 3.4.21.-
Gene Name prb1
Organism Hypocrea atroviridis (Trichoderma atroviride)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Hypocreaceae Trichoderma Hypocrea atroviridis (Trichoderma atroviride)
Enzyme Sequence MTSIRRLALYLGALLPAVLAAPAALHKKPEAVPNKFIVTLKEGASIDTDSHLAWVTDIHTRSLTKRSTAGVEKTYNIHTWNAYAGEFDEETIEQIKSNPDVASVEPDYIMHLSDIVEDKRALTTQSGAPWGLGTVSHRTSGSTSYIYDSSAGAGTFAYVVDSGINTSHQQFGGRASLGYNAAGGQHVDTLGHGTHVSGTIGGSTYGVAKQASLISVKVFAGESASTSVILDGYNWAVNDIVSKSRASKSAINMSLGGPASSTWTTAINAAFNQGVLTIVAAGNGDSLGNPQPVSGTSPANVPNAITVAALDINWRTASFTNYGAGVDVFAPGVNILSSWIGSNTATNTISGTSMATPHVVGLALYLQSLEGLTSPTAVTNRIKALATSGRVTGSLNGSPNVIIFNGNSS
Enzyme Length 409
Uniprot Accession Number Q03420
Absorption
Active Site ACT_SITE 161; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 192; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 353; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.21.-
Enzyme Function FUNCTION: Serine protease, secreted specifically during the mycoparasitic process, which is involved in the degradation of phytopathogen cell walls, membranes and of the proteins released after lysis of the host.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Domain (2); Glycosylation (1); Propeptide (1); Signal peptide (1)
Keywords Cleavage on pair of basic residues;Direct protein sequencing;Glycoprotein;Hydrolase;Protease;Secreted;Serine protease;Signal;Zymogen
Interact With
Induction INDUCTION: By mycelia, fungal cell walls, and chitin, but only in the absence of glucose.
Subcellular Location SUBCELLULAR LOCATION: Secreted.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..20; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 42,271
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda