Detail Information for IndEnz0002019347
IED ID IndEnz0002019347
Enzyme Type ID protease019347
Protein Name Thiol protease aleurain
EC 3.4.22.16
Gene Name
Organism Hordeum vulgare (Barley)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Pooideae Triticodae Triticeae Hordeinae Hordeum Hordeum vulgare (Barley)
Enzyme Sequence MAHARVLLLALAVLATAAVAVASSSSFADSNPIRPVTDRAASTLESAVLGALGRTRHALRFARFAVRYGKSYESAAEVRRRFRIFSESLEEVRSTNRKGLPYRLGINRFSDMSWEEFQATRLGAAQTCSATLAGNHLMRDAAALPETKDWREDGIVSPVKNQAHCGSCWTFSTTGALEAAYTQATGKNISLSEQQLVDCAGGFNNFGCNGGLPSQAFEYIKYNGGIDTEESYPYKGVNGVCHYKAENAAVQVLDSVNITLNAEDELKNAVGLVRPVSVAFQVIDGFRQYKSGVYTSDHCGTTPDDVNHAVLAVGYGVENGVPYWLIKNSWGADWGDNGYFKMEMGKNMCAIATCASYPVVAA
Enzyme Length 362
Uniprot Accession Number P05167
Absorption
Active Site ACT_SITE 168; /evidence=ECO:0000250; ACT_SITE 308; /evidence=ECO:0000250; ACT_SITE 328; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase.; EC=3.4.22.16;
DNA Binding
EC Number 3.4.22.16
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (3); Glycosylation (2); Propeptide (1); Signal peptide (1)
Keywords Disulfide bond;Germination;Glycoprotein;Hydrolase;Protease;Signal;Thiol protease;Vacuole;Zymogen
Interact With
Induction INDUCTION: Aleurain is synthesized by the aleurone cells stimulated by gibberellic or abscisic acid.
Subcellular Location SUBCELLULAR LOCATION: Vacuole. Note=Vacuole-like subcellular compartment.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..22; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 39,122
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda