| IED ID | IndEnz0002019347 |
| Enzyme Type ID | protease019347 |
| Protein Name |
Thiol protease aleurain EC 3.4.22.16 |
| Gene Name | |
| Organism | Hordeum vulgare (Barley) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Pooideae Triticodae Triticeae Hordeinae Hordeum Hordeum vulgare (Barley) |
| Enzyme Sequence | MAHARVLLLALAVLATAAVAVASSSSFADSNPIRPVTDRAASTLESAVLGALGRTRHALRFARFAVRYGKSYESAAEVRRRFRIFSESLEEVRSTNRKGLPYRLGINRFSDMSWEEFQATRLGAAQTCSATLAGNHLMRDAAALPETKDWREDGIVSPVKNQAHCGSCWTFSTTGALEAAYTQATGKNISLSEQQLVDCAGGFNNFGCNGGLPSQAFEYIKYNGGIDTEESYPYKGVNGVCHYKAENAAVQVLDSVNITLNAEDELKNAVGLVRPVSVAFQVIDGFRQYKSGVYTSDHCGTTPDDVNHAVLAVGYGVENGVPYWLIKNSWGADWGDNGYFKMEMGKNMCAIATCASYPVVAA |
| Enzyme Length | 362 |
| Uniprot Accession Number | P05167 |
| Absorption | |
| Active Site | ACT_SITE 168; /evidence=ECO:0000250; ACT_SITE 308; /evidence=ECO:0000250; ACT_SITE 328; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase.; EC=3.4.22.16; |
| DNA Binding | |
| EC Number | 3.4.22.16 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3); Glycosylation (2); Propeptide (1); Signal peptide (1) |
| Keywords | Disulfide bond;Germination;Glycoprotein;Hydrolase;Protease;Signal;Thiol protease;Vacuole;Zymogen |
| Interact With | |
| Induction | INDUCTION: Aleurain is synthesized by the aleurone cells stimulated by gibberellic or abscisic acid. |
| Subcellular Location | SUBCELLULAR LOCATION: Vacuole. Note=Vacuole-like subcellular compartment. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..22; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 39,122 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |