Detail Information for IndEnz0002019354
IED ID IndEnz0002019354
Enzyme Type ID protease019354
Protein Name Probable cytosol aminopeptidase
EC 3.4.11.1
Leucine aminopeptidase
LAP
EC 3.4.11.10
Leucyl aminopeptidase
Gene Name pepA Mrad2831_0557
Organism Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Alphaproteobacteria Hyphomicrobiales Methylobacteriaceae Methylobacterium Methylobacterium radiotolerans Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1)
Enzyme Sequence MADGIEIGFGPLEQSGQGPGGSGDLVVFVGDDLSLGSAAREALGASGADLVARAAASEKFKGRSLSALSLPAPAGVGADRLVVVGLGSEKDRAKTDWPALGGFTASKVAGRTARVVLDWPGTTVTAAQAGEFALGARLRTYAFDRYKTKKKPDADDKSVTALTLLLADHGAAAREGEGARSLSDGVILARDLVNEPPNVLFPAEFARRASELAKLGVEIEVLEPARMRELGMGALLAVAQGSAREPRIVIMRWNGGPAAEAPVALIGKGVVFDSGGVSIKPGGGMEDMKGDMGGAAAVVGALHALAARKARCNVVGAIGIVENMPDGGAYRPSDILTSMSGQTIEVINTDAEGRLVLADVITHVIRSTKPKAIVDLATLTGAIIVALGQDIAGMFSNDDTLASNIHAAGEATGEKVWRMPLIPAYDKAIDSKFADMKNTGGRHGGAATAASFIKRYVEDVPWAHLDIAGVAMSSNASEINRSWGAGWGVRLLDRLIRDHYEAR
Enzyme Length 503
Uniprot Accession Number B1LVC3
Absorption
Active Site ACT_SITE 280; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 354; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181};
DNA Binding
EC Number 3.4.11.1; 3.4.11.10
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (7)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 52,045
Kinetics
Metal Binding METAL 268; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 273; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 273; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 291; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 350; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 352; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 352; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Rhea ID
Cross Reference Brenda