Detail Information for IndEnz0002019356
IED ID IndEnz0002019356
Enzyme Type ID protease019356
Protein Name Probable cytosol aminopeptidase
EC 3.4.11.1
Leucine aminopeptidase
LAP
EC 3.4.11.10
Leucyl aminopeptidase
Gene Name pepA Mkms_3372
Organism Mycobacterium sp. (strain KMS)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycobacterium unclassified Mycobacterium Mycobacterium sp. (strain KMS)
Enzyme Sequence MSSDPGYQAPVVTVSSSIPRRGVGDSVLIVPVVTRDDAAAVLAAAPFLDKDAVREIEAALKSLGATGGEGQTHRLVVSALPVASVLTIGLGKERDEWPADTVRRVAGNAARSLDKVAAVLTTLSALDLEAAIEGLILGSYRFTEFRSAKTAPKDGGLRAITALSQESKSRARDAAQRATDIATAVATARDFVNTPPSHLYPDEFAKRAKALGEAAGLEVEILDDKALVKAGYGGIVGVGKGSSRPPRLVRLSHKGAVRTRTRGARPGGSKRVALVGKGITFDTGGISIKPAANMHHMTSDMGGAAAVIATVVLAAKQKLPIDVIATVPMAENMPSATAQRPGDVLTQYGGTTVEVLNTDAEGRLILADAIVRACEDNPDYLIETSTLTGAQTVALGSRTPGVMGSDAFRDRVATLSQQVGENAWAMPLPEELKDDLKSTVADLANVSGSRFAGMLVAGTYLREFVADGVEWAHIDVAAPAYNSGGPWGYTPKGGTGVPTRTMFAVLEEIAREG
Enzyme Length 513
Uniprot Accession Number A1UIA8
Absorption
Active Site ACT_SITE 289; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 363; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181};
DNA Binding
EC Number 3.4.11.1; 3.4.11.10
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (7)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 53,339
Kinetics
Metal Binding METAL 277; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 282; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 282; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 300; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 359; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 361; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 361; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Rhea ID
Cross Reference Brenda