Detail Information for IndEnz0002019359
IED ID IndEnz0002019359
Enzyme Type ID protease019359
Protein Name Probable cytosol aminopeptidase
EC 3.4.11.1
Leucine aminopeptidase
LAP
EC 3.4.11.10
Leucyl aminopeptidase
Gene Name pepA PMT_1407
Organism Prochlorococcus marinus (strain MIT 9313)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Cyanobacteria/Melainabacteria group Cyanobacteria Synechococcales Prochlorococcaceae Prochlorococcus Prochlorococcus marinus Prochlorococcus marinus (strain MIT 9313)
Enzyme Sequence MEICLFPATLQSWTGDVLMVGMFEGKMEERLNELETLCKGSLMQSLEKQMFKGKSGEIATVQLLQNKPNLLVLVGLGEPQEMRLDDLRKAAALGAKASLGCSGTLGMMLPWEPLDAASAARAVAEAVRLSLYKDLRFRSAPEPRSTPTKLELIGLPDSAGKDLQAVHPTCAGVELARQLVAAPANSLTPAALAQTAIQLAHEHGLECSVLERSDCAEREMGAYLAVSQGSDLEPKFIHLTYRPQGPVQRRLALVGKGLTFDSGGYNLKVGAAQIDLMKFDMGGSAAVLGAARAIAELRPKGVEVHVIVAACENMVNGSAVHPGDLVRASNGTTIEINNTDAEGRLTLADALVYTCGLEPDAIVDLATLTGACVIALGEEIAGLWTGHDPLAEGLTAAAEAAGEGLWRMPLPSSYREGLKSNLADLKNTGPRPGGSITAALFLKEFVEASIPWAHIDIAGTVWSEKGRGLNPSGATGYGVRTLVNWICSQS
Enzyme Length 490
Uniprot Accession Number Q7V5X8
Absorption
Active Site ACT_SITE 268; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 344; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181};
DNA Binding
EC Number 3.4.11.1; 3.4.11.10
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (7)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 51,728
Kinetics
Metal Binding METAL 256; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 261; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 261; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 280; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 340; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 342; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 342; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Rhea ID
Cross Reference Brenda